A novel nanostructured biosensor for the detection of the dust mite antigen Der p2

Jaw-Ji Tsai1, I-Jiuan Bau2, He-Tsing Chen2, Yu-Ta Lin3, Gou-Jen Wang2,31Department of Medical Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan; 2Graduate Institute of Biomedical Engineering, 3Department of Mechanical Engineering, National Chung-Hsing University, Taichung,...

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Autores principales:	Tsai JJ, Bau IJ, Chen HT, Lin YT, Wang GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eee85d01943f4a74a79b08ff8f2c1b18
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spelling	oai:doaj.org-article:eee85d01943f4a74a79b08ff8f2c1b182021-12-02T06:38:00ZA novel nanostructured biosensor for the detection of the dust mite antigen Der p21176-91141178-2013https://doaj.org/article/eee85d01943f4a74a79b08ff8f2c1b182011-06-01T00:00:00Zhttp://www.dovepress.com/a-novel-nanostructured-biosensor-for-the-detection-of-the-dust-mite-an-a7641https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jaw-Ji Tsai1, I-Jiuan Bau2, He-Tsing Chen2, Yu-Ta Lin3, Gou-Jen Wang2,31Department of Medical Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan; 2Graduate Institute of Biomedical Engineering, 3Department of Mechanical Engineering, National Chung-Hsing University, Taichung, TaiwanAbstract: The group 2 allergen, Der p2, has been reported to activate innate toll-like receptors (TLRs) on respiratory epithelial cells and thus aggravate respiratory diseases. In this study, a highly sensitive nanostructured biosensor based on a 3D sensing element with uniformly deposited gold nanoparticles is proposed for the detection of the dust mite antigen Der p2. The barrier layer comprises an anodic aluminum oxide (AAO) film which is used as the template in this highly sensitive nanostructured biosensor. Simple electrochemical deposition without reducing agent and stabilizer was enough to uniformly synthesize gold nanoparticles on the surface of the barrier layer. The size and the distribution density of the nanoparticles can be well controlled by the applied potential during electrochemical deposition. Following this procedure, the dust mite monoclonal antibodies (IgG) were then immobilized through the 11-MUA (11-mercaptoundecanoic acid), (1-ethyl-3-(3-dimethyl-aminopropyl)-carbodiimide)/(N-hydroxysuccinimide) self-assembled monolayer approach for the dust mite antigen Der p2 detection. The detection limit of the proposed 3D gold nanoparticle-based nanostructured biosensor was examined using electrochemical impedance spectroscopy analysis and found to be 1 pg/mL. The dynamic range was found to be 5 &micro;g/mL. The proposed nanostructured biosensor would be useful for fast detection of rare molecules in a solution.Keywords: anodic aluminum oxide template, gold nanoparticles, nanostructured biosensor, Der p2 detection, electrochemical impedance spectroscopy analysisTsai JJBau IJChen HTLin YTWang GJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1201-1208 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tsai JJ Bau IJ Chen HT Lin YT Wang GJ A novel nanostructured biosensor for the detection of the dust mite antigen Der p2
description	Jaw-Ji Tsai1, I-Jiuan Bau2, He-Tsing Chen2, Yu-Ta Lin3, Gou-Jen Wang2,31Department of Medical Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan; 2Graduate Institute of Biomedical Engineering, 3Department of Mechanical Engineering, National Chung-Hsing University, Taichung, TaiwanAbstract: The group 2 allergen, Der p2, has been reported to activate innate toll-like receptors (TLRs) on respiratory epithelial cells and thus aggravate respiratory diseases. In this study, a highly sensitive nanostructured biosensor based on a 3D sensing element with uniformly deposited gold nanoparticles is proposed for the detection of the dust mite antigen Der p2. The barrier layer comprises an anodic aluminum oxide (AAO) film which is used as the template in this highly sensitive nanostructured biosensor. Simple electrochemical deposition without reducing agent and stabilizer was enough to uniformly synthesize gold nanoparticles on the surface of the barrier layer. The size and the distribution density of the nanoparticles can be well controlled by the applied potential during electrochemical deposition. Following this procedure, the dust mite monoclonal antibodies (IgG) were then immobilized through the 11-MUA (11-mercaptoundecanoic acid), (1-ethyl-3-(3-dimethyl-aminopropyl)-carbodiimide)/(N-hydroxysuccinimide) self-assembled monolayer approach for the dust mite antigen Der p2 detection. The detection limit of the proposed 3D gold nanoparticle-based nanostructured biosensor was examined using electrochemical impedance spectroscopy analysis and found to be 1 pg/mL. The dynamic range was found to be 5 &micro;g/mL. The proposed nanostructured biosensor would be useful for fast detection of rare molecules in a solution.Keywords: anodic aluminum oxide template, gold nanoparticles, nanostructured biosensor, Der p2 detection, electrochemical impedance spectroscopy analysis
format	article
author	Tsai JJ Bau IJ Chen HT Lin YT Wang GJ
author_facet	Tsai JJ Bau IJ Chen HT Lin YT Wang GJ
author_sort	Tsai JJ
title	A novel nanostructured biosensor for the detection of the dust mite antigen Der p2
title_short	A novel nanostructured biosensor for the detection of the dust mite antigen Der p2
title_full	A novel nanostructured biosensor for the detection of the dust mite antigen Der p2
title_fullStr	A novel nanostructured biosensor for the detection of the dust mite antigen Der p2
title_full_unstemmed	A novel nanostructured biosensor for the detection of the dust mite antigen Der p2
title_sort	novel nanostructured biosensor for the detection of the dust mite antigen der p2
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/eee85d01943f4a74a79b08ff8f2c1b18
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A novel nanostructured biosensor for the detection of the dust mite antigen Der p2

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