Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.

Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens that can cause serious infections ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS). Translocation of Shiga-toxins (Stx) from the gut lumen to underlying tissues is a decisive step in the developme...

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Autores principales:	Lucie Etienne-Mesmin, Benoit Chassaing, Pierre Sauvanet, Jérémy Denizot, Stéphanie Blanquet-Diot, Arlette Darfeuille-Michaud, Nathalie Pradel, Valérie Livrelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eee8a97f948e4cb193e7cc1786c86403
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spelling	oai:doaj.org-article:eee8a97f948e4cb193e7cc1786c864032021-11-18T06:47:46ZInteractions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.1932-620310.1371/journal.pone.0023594https://doaj.org/article/eee8a97f948e4cb193e7cc1786c864032011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21858177/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens that can cause serious infections ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS). Translocation of Shiga-toxins (Stx) from the gut lumen to underlying tissues is a decisive step in the development of the infection, but the mechanisms involved remain unclear. Many bacterial pathogens target the follicle-associated epithelium, which overlies Peyer's patches (PPs), cross the intestinal barrier through M cells and are captured by mucosal macrophages. Here, translocation across M cells, as well as survival and proliferation of EHEC strains within THP-1 macrophages were investigated using EHEC O157:H7 reference strains, isogenic mutants, and 15 EHEC strains isolated from HC/HUS patients. We showed for the first time that E. coli O157:H7 strains are able to interact in vivo with murine PPs, to translocate ex vivo through murine ileal mucosa with PPs and across an in vitro human M cell model. EHEC strains are also able to survive and to produce Stx in macrophages, which induce cell apoptosis and Stx release. In conclusion, our results suggest that the uptake of EHEC by M cells and underlying macrophages in the PP may be a critical step in Stx translocation and release in vivo. A new model for EHEC infection in humans is proposed that could help in a fuller understanding of EHEC-associated diseases.Lucie Etienne-MesminBenoit ChassaingPierre SauvanetJérémy DenizotStéphanie Blanquet-DiotArlette Darfeuille-MichaudNathalie PradelValérie LivrelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23594 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucie Etienne-Mesmin Benoit Chassaing Pierre Sauvanet Jérémy Denizot Stéphanie Blanquet-Diot Arlette Darfeuille-Michaud Nathalie Pradel Valérie Livrelli Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.
description	Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens that can cause serious infections ranging from diarrhea to hemorrhagic colitis (HC) and hemolytic-uremic syndrome (HUS). Translocation of Shiga-toxins (Stx) from the gut lumen to underlying tissues is a decisive step in the development of the infection, but the mechanisms involved remain unclear. Many bacterial pathogens target the follicle-associated epithelium, which overlies Peyer's patches (PPs), cross the intestinal barrier through M cells and are captured by mucosal macrophages. Here, translocation across M cells, as well as survival and proliferation of EHEC strains within THP-1 macrophages were investigated using EHEC O157:H7 reference strains, isogenic mutants, and 15 EHEC strains isolated from HC/HUS patients. We showed for the first time that E. coli O157:H7 strains are able to interact in vivo with murine PPs, to translocate ex vivo through murine ileal mucosa with PPs and across an in vitro human M cell model. EHEC strains are also able to survive and to produce Stx in macrophages, which induce cell apoptosis and Stx release. In conclusion, our results suggest that the uptake of EHEC by M cells and underlying macrophages in the PP may be a critical step in Stx translocation and release in vivo. A new model for EHEC infection in humans is proposed that could help in a fuller understanding of EHEC-associated diseases.
format	article
author	Lucie Etienne-Mesmin Benoit Chassaing Pierre Sauvanet Jérémy Denizot Stéphanie Blanquet-Diot Arlette Darfeuille-Michaud Nathalie Pradel Valérie Livrelli
author_facet	Lucie Etienne-Mesmin Benoit Chassaing Pierre Sauvanet Jérémy Denizot Stéphanie Blanquet-Diot Arlette Darfeuille-Michaud Nathalie Pradel Valérie Livrelli
author_sort	Lucie Etienne-Mesmin
title	Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.
title_short	Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.
title_full	Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.
title_fullStr	Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.
title_full_unstemmed	Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.
title_sort	interactions with m cells and macrophages as key steps in the pathogenesis of enterohemorrhagic escherichia coli infections.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/eee8a97f948e4cb193e7cc1786c86403
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Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.

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