Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle

Abstract We previously reported that intramuscular injections of ubiquitin ligase CBLB inhibitory pentapeptide (Cblin; Asp-Gly-pTyr-Met-Pro) restored lost muscle mass caused by sciatic denervation. Here, we detected Cblin on the basolateral side of Caco-2 cells after being placed on the apical side,...

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Autores principales:	Reiko Nakao, Weilin Shen, Yasuka Shimajiri, Kumiko Kainou, Yuki Sato, Anayt Ulla, Kohta Ohnishi, Miyuki Ninomiya, Ayako Ohno, Takayuki Uchida, Mitsuru Tanaka, Kazuhito Akama, Toshiro Matsui, Takeshi Nikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/eeeb33b38b5a48a3b5ac9df76e7ee2fd
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spelling	oai:doaj.org-article:eeeb33b38b5a48a3b5ac9df76e7ee2fd2021-12-02T14:54:28ZOral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle10.1038/s41538-021-00108-02396-8370https://doaj.org/article/eeeb33b38b5a48a3b5ac9df76e7ee2fd2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41538-021-00108-0https://doaj.org/toc/2396-8370Abstract We previously reported that intramuscular injections of ubiquitin ligase CBLB inhibitory pentapeptide (Cblin; Asp-Gly-pTyr-Met-Pro) restored lost muscle mass caused by sciatic denervation. Here, we detected Cblin on the basolateral side of Caco-2 cells after being placed on the apical side, and found that cytochalasin D, a tight junction opener, enhanced Cblin transport. Orally administered Cblin was found in rat plasma, indicating that intact Cblin was absorbed in vitro and in vivo. Furthermore, transgenic Cblin peptide-enriched rice (CbR) prevented the denervation-induced loss of muscle mass and the upregulation of muscle atrophy-related ubiquitin ligases in mice. These findings indicated that CbR could serve as an alternative treatment for muscle atrophy.Reiko NakaoWeilin ShenYasuka ShimajiriKumiko KainouYuki SatoAnayt UllaKohta OhnishiMiyuki NinomiyaAyako OhnoTakayuki UchidaMitsuru TanakaKazuhito AkamaToshiro MatsuiTakeshi NikawaNature PortfolioarticleNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENnpj Science of Food, Vol 5, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Reiko Nakao Weilin Shen Yasuka Shimajiri Kumiko Kainou Yuki Sato Anayt Ulla Kohta Ohnishi Miyuki Ninomiya Ayako Ohno Takayuki Uchida Mitsuru Tanaka Kazuhito Akama Toshiro Matsui Takeshi Nikawa Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
description	Abstract We previously reported that intramuscular injections of ubiquitin ligase CBLB inhibitory pentapeptide (Cblin; Asp-Gly-pTyr-Met-Pro) restored lost muscle mass caused by sciatic denervation. Here, we detected Cblin on the basolateral side of Caco-2 cells after being placed on the apical side, and found that cytochalasin D, a tight junction opener, enhanced Cblin transport. Orally administered Cblin was found in rat plasma, indicating that intact Cblin was absorbed in vitro and in vivo. Furthermore, transgenic Cblin peptide-enriched rice (CbR) prevented the denervation-induced loss of muscle mass and the upregulation of muscle atrophy-related ubiquitin ligases in mice. These findings indicated that CbR could serve as an alternative treatment for muscle atrophy.
format	article
author	Reiko Nakao Weilin Shen Yasuka Shimajiri Kumiko Kainou Yuki Sato Anayt Ulla Kohta Ohnishi Miyuki Ninomiya Ayako Ohno Takayuki Uchida Mitsuru Tanaka Kazuhito Akama Toshiro Matsui Takeshi Nikawa
author_facet	Reiko Nakao Weilin Shen Yasuka Shimajiri Kumiko Kainou Yuki Sato Anayt Ulla Kohta Ohnishi Miyuki Ninomiya Ayako Ohno Takayuki Uchida Mitsuru Tanaka Kazuhito Akama Toshiro Matsui Takeshi Nikawa
author_sort	Reiko Nakao
title	Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
title_short	Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
title_full	Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
title_fullStr	Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
title_full_unstemmed	Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
title_sort	oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eeeb33b38b5a48a3b5ac9df76e7ee2fd
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Oral intake of rice overexpressing ubiquitin ligase inhibitory pentapeptide prevents atrophy in denervated skeletal muscle

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