Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film

Polyvinyl alcohol (PVA) hydrogel is one of the candidate materials for artificial cartilage. In previous research, it was found that the structure of protein boundary film is important essence for wear reduction of PVA hydrogel, and the stability of protein boundary film changes with relative ratio...

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Autores principales:	Seido Yarimitsu, Kazuhiro Nakashima, Yoshinori Sawae, Teruo Murakami
Formato:	article
Lenguaje:	EN
Publicado:	Japanese Society of Tribologists 2008
Materias:	artificial cartilage adsorption protein boundary film total internal reflection microscopy Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/eef5d3da3bcc4a26a0d8b4fef02a408b
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spelling	oai:doaj.org-article:eef5d3da3bcc4a26a0d8b4fef02a408b2021-11-05T09:28:59ZEffects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film1881-219810.2474/trol.3.238https://doaj.org/article/eef5d3da3bcc4a26a0d8b4fef02a408b2008-08-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/trol/3/4/3_4_238/_pdf/-char/enhttps://doaj.org/toc/1881-2198Polyvinyl alcohol (PVA) hydrogel is one of the candidate materials for artificial cartilage. In previous research, it was found that the structure of protein boundary film is important essence for wear reduction of PVA hydrogel, and the stability of protein boundary film changes with relative ratio and total concentration of proteins. In this study, the behavior of protein adsorption on the glass surface rubbed with PVA hydrogel was observed by using total internal reflection fluorescence microscopy (TIRFM). Amount of adsorbed proteins under rubbed condition increased compared to those under statically immersed condition. In lubricant in which a stable protein boundary film was formed, the amount of adsorbed proteins in bottom layer of protein boundary film increased significantly with an increase of sliding distance. In addition, the ratio of adsorbed immuno globulin G (IgG) to albumin was higher than that in lubricant in which an exfoliative protein boundary film was formed. These results indicate that the formation of bottom layer mainly composed of γ-globulin is a critical element to form a stable boundary film, and the friction-induced enhancement of forming protein boundary film may occur in lubricant with appropriate protein constituents.Seido YarimitsuKazuhiro NakashimaYoshinori SawaeTeruo MurakamiJapanese Society of Tribologistsarticleartificial cartilageadsorptionprotein boundary filmtotal internal reflection microscopyPhysicsQC1-999Engineering (General). Civil engineering (General)TA1-2040Mechanical engineering and machineryTJ1-1570ChemistryQD1-999ENTribology Online, Vol 3, Iss 4, Pp 238-242 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	artificial cartilage adsorption protein boundary film total internal reflection microscopy Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999
spellingShingle	artificial cartilage adsorption protein boundary film total internal reflection microscopy Physics QC1-999 Engineering (General). Civil engineering (General) TA1-2040 Mechanical engineering and machinery TJ1-1570 Chemistry QD1-999 Seido Yarimitsu Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film
description	Polyvinyl alcohol (PVA) hydrogel is one of the candidate materials for artificial cartilage. In previous research, it was found that the structure of protein boundary film is important essence for wear reduction of PVA hydrogel, and the stability of protein boundary film changes with relative ratio and total concentration of proteins. In this study, the behavior of protein adsorption on the glass surface rubbed with PVA hydrogel was observed by using total internal reflection fluorescence microscopy (TIRFM). Amount of adsorbed proteins under rubbed condition increased compared to those under statically immersed condition. In lubricant in which a stable protein boundary film was formed, the amount of adsorbed proteins in bottom layer of protein boundary film increased significantly with an increase of sliding distance. In addition, the ratio of adsorbed immuno globulin G (IgG) to albumin was higher than that in lubricant in which an exfoliative protein boundary film was formed. These results indicate that the formation of bottom layer mainly composed of γ-globulin is a critical element to form a stable boundary film, and the friction-induced enhancement of forming protein boundary film may occur in lubricant with appropriate protein constituents.
format	article
author	Seido Yarimitsu Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami
author_facet	Seido Yarimitsu Kazuhiro Nakashima Yoshinori Sawae Teruo Murakami
author_sort	Seido Yarimitsu
title	Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film
title_short	Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film
title_full	Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film
title_fullStr	Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film
title_full_unstemmed	Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film
title_sort	effects of lubricant composition on adsorption behavior of proteins on rubbing surface and stability of protein boundary film
publisher	Japanese Society of Tribologists
publishDate	2008
url	https://doaj.org/article/eef5d3da3bcc4a26a0d8b4fef02a408b
work_keys_str_mv	AT seidoyarimitsu effectsoflubricantcompositiononadsorptionbehaviorofproteinsonrubbingsurfaceandstabilityofproteinboundaryfilm AT kazuhironakashima effectsoflubricantcompositiononadsorptionbehaviorofproteinsonrubbingsurfaceandstabilityofproteinboundaryfilm AT yoshinorisawae effectsoflubricantcompositiononadsorptionbehaviorofproteinsonrubbingsurfaceandstabilityofproteinboundaryfilm AT teruomurakami effectsoflubricantcompositiononadsorptionbehaviorofproteinsonrubbingsurfaceandstabilityofproteinboundaryfilm
_version_	1718444384228212736

Effects of Lubricant Composition on Adsorption Behavior of Proteins on Rubbing Surface and Stability of Protein Boundary Film

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