The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models

Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplement...

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Autores principales:	Puspita Kusuma Dewi, Maharani Cahyono, Riski Prihatningtias, Liana Ekowati, Arief Wildan
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2020
Materias:	retinopathy of prematurity glutathione malondialdehyde Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/eef5ef5aadf14ffca096d18eee0c9a97
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spelling	oai:doaj.org-article:eef5ef5aadf14ffca096d18eee0c9a972021-11-05T16:47:43ZThe Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models2503-217810.14710/jbtr.v6i3.9414https://doaj.org/article/eef5ef5aadf14ffca096d18eee0c9a972020-12-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/9414https://doaj.org/toc/2503-2178Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models. Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit. Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001). Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.Puspita Kusuma DewiMaharani CahyonoRiski PrihatningtiasLiana EkowatiArief WildanDiponegoro Universityarticleretinopathy of prematurityglutathionemalondialdehydeMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 6, Iss 3, Pp 86-88 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	retinopathy of prematurity glutathione malondialdehyde Medicine (General) R5-920
spellingShingle	retinopathy of prematurity glutathione malondialdehyde Medicine (General) R5-920 Puspita Kusuma Dewi Maharani Cahyono Riski Prihatningtias Liana Ekowati Arief Wildan The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models
description	Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models. Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit. Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001). Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.
format	article
author	Puspita Kusuma Dewi Maharani Cahyono Riski Prihatningtias Liana Ekowati Arief Wildan
author_facet	Puspita Kusuma Dewi Maharani Cahyono Riski Prihatningtias Liana Ekowati Arief Wildan
author_sort	Puspita Kusuma Dewi
title	The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models
title_short	The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models
title_full	The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models
title_fullStr	The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models
title_full_unstemmed	The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models
title_sort	effect of glutathione on serum malondialdehyde (mda) level in retinopathy of prematurity rat models
publisher	Diponegoro University
publishDate	2020
url	https://doaj.org/article/eef5ef5aadf14ffca096d18eee0c9a97
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The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models

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