MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line
Abstract NRAS mutation in melanoma has been associated with aggressive tumor biology and poor prognosis. Although targeted therapy has been tested for NRAS mutated melanoma, response rates still appear much weaker, than in BRAF mutated melanoma. While plenty of cell lines exist, however, only few me...
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oai:doaj.org-article:eef9e4f4dce249289106e63a22159f422021-12-02T15:06:17ZMUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line10.1038/s41598-017-02197-y2045-2322https://doaj.org/article/eef9e4f4dce249289106e63a22159f422017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02197-yhttps://doaj.org/toc/2045-2322Abstract NRAS mutation in melanoma has been associated with aggressive tumor biology and poor prognosis. Although targeted therapy has been tested for NRAS mutated melanoma, response rates still appear much weaker, than in BRAF mutated melanoma. While plenty of cell lines exist, however, only few melanogenic cell lines retain their in vivo characteristics. In this work we present an intensively pigmented and well-characterized cell line derived from a highly aggressive NRAS mutated cutaneous melanoma, named MUG-Mel2. We present the clinical course, unique morphology, angiogenic properties, growth characteristics using in vivo experiments and 3D cell culture, and results of the exome gene sequencing of an intensively pigmented melanogenic cell line MUG-Mel2, derived from a cutaneous metastasis of an aggressive NRAS p. Q61R mutated melanoma. Amongst several genetic alterations, mutations in GRIN2A, CREBP, PIK3C2G, ATM, and ATR were present. These mutations, known to reinforce DNA repair problems in melanoma, might serve as potential treatment targets. The aggressive and fast growing behavior in animal models and the obtained phenotype in 3D culture reveal a perfect model for research in the field of NRAS mutated melanoma.Beate RinnerGreta GandolfiKatharina MeditzMarie-Therese FrischKarin WagnerAlessia CiarrocchiFederica TorricelliRaili KoivuniemiJohanna NiklanderBernadette Liegl-AtzwangerBirgit LohbergerEllen HeitzerNassim Ghaffari-Tabrizi-WizsyDagmar ZweytickIris ZalaudekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Beate Rinner Greta Gandolfi Katharina Meditz Marie-Therese Frisch Karin Wagner Alessia Ciarrocchi Federica Torricelli Raili Koivuniemi Johanna Niklander Bernadette Liegl-Atzwanger Birgit Lohberger Ellen Heitzer Nassim Ghaffari-Tabrizi-Wizsy Dagmar Zweytick Iris Zalaudek MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line |
| description |
Abstract NRAS mutation in melanoma has been associated with aggressive tumor biology and poor prognosis. Although targeted therapy has been tested for NRAS mutated melanoma, response rates still appear much weaker, than in BRAF mutated melanoma. While plenty of cell lines exist, however, only few melanogenic cell lines retain their in vivo characteristics. In this work we present an intensively pigmented and well-characterized cell line derived from a highly aggressive NRAS mutated cutaneous melanoma, named MUG-Mel2. We present the clinical course, unique morphology, angiogenic properties, growth characteristics using in vivo experiments and 3D cell culture, and results of the exome gene sequencing of an intensively pigmented melanogenic cell line MUG-Mel2, derived from a cutaneous metastasis of an aggressive NRAS p. Q61R mutated melanoma. Amongst several genetic alterations, mutations in GRIN2A, CREBP, PIK3C2G, ATM, and ATR were present. These mutations, known to reinforce DNA repair problems in melanoma, might serve as potential treatment targets. The aggressive and fast growing behavior in animal models and the obtained phenotype in 3D culture reveal a perfect model for research in the field of NRAS mutated melanoma. |
| format |
article |
| author |
Beate Rinner Greta Gandolfi Katharina Meditz Marie-Therese Frisch Karin Wagner Alessia Ciarrocchi Federica Torricelli Raili Koivuniemi Johanna Niklander Bernadette Liegl-Atzwanger Birgit Lohberger Ellen Heitzer Nassim Ghaffari-Tabrizi-Wizsy Dagmar Zweytick Iris Zalaudek |
| author_facet |
Beate Rinner Greta Gandolfi Katharina Meditz Marie-Therese Frisch Karin Wagner Alessia Ciarrocchi Federica Torricelli Raili Koivuniemi Johanna Niklander Bernadette Liegl-Atzwanger Birgit Lohberger Ellen Heitzer Nassim Ghaffari-Tabrizi-Wizsy Dagmar Zweytick Iris Zalaudek |
| author_sort |
Beate Rinner |
| title |
MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line |
| title_short |
MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line |
| title_full |
MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line |
| title_fullStr |
MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line |
| title_full_unstemmed |
MUG-Mel2, a novel highly pigmented and well characterized NRAS mutated human melanoma cell line |
| title_sort |
mug-mel2, a novel highly pigmented and well characterized nras mutated human melanoma cell line |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/eef9e4f4dce249289106e63a22159f42 |
| work_keys_str_mv |
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