Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

Abstract Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide. Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluati...

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Autores principales:	Xiaocui Bian, Fang Cao, Xiaowan Wang, Yuhong Hou, Haitao Zhao, Yuqin Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eefb19d8bb484f38976589931e9e83e4
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spelling	oai:doaj.org-article:eefb19d8bb484f38976589931e9e83e42021-12-02T18:02:54ZEstablishment and characterization of a new human colon cancer cell line, PUMC-CRC110.1038/s41598-021-92491-72045-2322https://doaj.org/article/eefb19d8bb484f38976589931e9e83e42021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92491-7https://doaj.org/toc/2045-2322Abstract Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide. Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluating antitumor drugs in CRC studies. Our de novo CRC cell line, PUMC-CRC1 (Peking Union Medical College Colorectal Cancer 1) was derived from a 47-year-old Chinese female patient diagnosed with moderately to poorly differentiated colon adenocarcinoma. Multiple experiments were used for full characterization. The new cell line was epithelial-like and was passaged for more than 40 times, with a population doubling time of 44 h in vitro, detected by cell counts. The cells exhibited complicated chromosomal abnormalities. The tumor formation rate in SCID mice was 100%. The xenograft tumor was adenocarcinoma with poor to moderate differentiation by Haematoxylin and Eosin staining (H&E) sections. Immunohistochemistry (IHC) analysis and next-generation sequencing (NGS) revealed microsatellite stable (MSS), APC (p.T1493fs) inactivation, KRAS (p.G12V) activation, and SMAD4 (p.V506A) mutation. Quality control of the cell line proved mycoplasma negative and identical STR profile with that of the original tissue, and no interspecific or intraspecific cross contamination was detected. In conclusion, PUMC-CRC1 was a newly established and well characterized human colon cancer cell line, which might be a good model for both in vitro and in vivo studies of the mechanism of colon cancer progression and the treatment strategies for MSS CRC.Xiaocui BianFang CaoXiaowan WangYuhong HouHaitao ZhaoYuqin LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaocui Bian Fang Cao Xiaowan Wang Yuhong Hou Haitao Zhao Yuqin Liu Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1
description	Abstract Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide. Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluating antitumor drugs in CRC studies. Our de novo CRC cell line, PUMC-CRC1 (Peking Union Medical College Colorectal Cancer 1) was derived from a 47-year-old Chinese female patient diagnosed with moderately to poorly differentiated colon adenocarcinoma. Multiple experiments were used for full characterization. The new cell line was epithelial-like and was passaged for more than 40 times, with a population doubling time of 44 h in vitro, detected by cell counts. The cells exhibited complicated chromosomal abnormalities. The tumor formation rate in SCID mice was 100%. The xenograft tumor was adenocarcinoma with poor to moderate differentiation by Haematoxylin and Eosin staining (H&E) sections. Immunohistochemistry (IHC) analysis and next-generation sequencing (NGS) revealed microsatellite stable (MSS), APC (p.T1493fs) inactivation, KRAS (p.G12V) activation, and SMAD4 (p.V506A) mutation. Quality control of the cell line proved mycoplasma negative and identical STR profile with that of the original tissue, and no interspecific or intraspecific cross contamination was detected. In conclusion, PUMC-CRC1 was a newly established and well characterized human colon cancer cell line, which might be a good model for both in vitro and in vivo studies of the mechanism of colon cancer progression and the treatment strategies for MSS CRC.
format	article
author	Xiaocui Bian Fang Cao Xiaowan Wang Yuhong Hou Haitao Zhao Yuqin Liu
author_facet	Xiaocui Bian Fang Cao Xiaowan Wang Yuhong Hou Haitao Zhao Yuqin Liu
author_sort	Xiaocui Bian
title	Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1
title_short	Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1
title_full	Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1
title_fullStr	Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1
title_full_unstemmed	Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1
title_sort	establishment and characterization of a new human colon cancer cell line, pumc-crc1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eefb19d8bb484f38976589931e9e83e4
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Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

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