Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.

Respiratory syncytial virus (RSV) causes acute exacerbations in COPD and asthma. RSV infects bronchial epithelial cells (HBE) that trigger RSV associated lung pathology. This study explores whether the phosphodiesterase 4 (PDE4) inhibitor Roflumilast N-oxide (RNO), alters RSV infection of well-diffe...

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Autores principales:	Manuel Mata, Isidoro Martinez, Jose A Melero, Herman Tenor, Julio Cortijo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eeffae9a83494ce4973afe8664160734
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spelling	oai:doaj.org-article:eeffae9a83494ce4973afe86641607342021-11-18T09:03:15ZRoflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.1932-620310.1371/journal.pone.0069670https://doaj.org/article/eeffae9a83494ce4973afe86641607342013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936072/?tool=EBIhttps://doaj.org/toc/1932-6203Respiratory syncytial virus (RSV) causes acute exacerbations in COPD and asthma. RSV infects bronchial epithelial cells (HBE) that trigger RSV associated lung pathology. This study explores whether the phosphodiesterase 4 (PDE4) inhibitor Roflumilast N-oxide (RNO), alters RSV infection of well-differentiated HBE (WD-HBE) in vitro. WD-HBE were RSV infected in the presence or absence of RNO (0.1-100 nM). Viral infection (staining of F and G proteins, nucleoprotein RNA level), mRNA of ICAM-1, ciliated cell markers (digital high speed videomicroscopy, β-tubulin immunofluorescence, Foxj1 and Dnai2 mRNA), Goblet cells (PAS), mRNA of MUC5AC and CLCA1, mRNA and protein level of IL-13, IL-6, IL-8, TNFα, formation of H2O2 and the anti-oxidative armamentarium (mRNA of Nrf2, HO-1, GPx; total antioxidant capacity (TAC) were measured at day 10 or 15 post infection. RNO inhibited RSV infection of WD-HBE, prevented the loss of ciliated cells and markers, reduced the increase of MUC5AC and CLCA1 and inhibited the increase of IL-13, IL-6, IL-8, TNFα and ICAM-1. Additionally RNO reversed the reduction of Nrf2, HO-1 and GPx mRNA levels and consequently restored the TAC and reduced the H2O2 formation. RNO inhibits RSV infection of WD-HBE cultures and mitigates the cytopathological changes associated to this virus.Manuel MataIsidoro MartinezJose A MeleroHerman TenorJulio CortijoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69670 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuel Mata Isidoro Martinez Jose A Melero Herman Tenor Julio Cortijo Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
description	Respiratory syncytial virus (RSV) causes acute exacerbations in COPD and asthma. RSV infects bronchial epithelial cells (HBE) that trigger RSV associated lung pathology. This study explores whether the phosphodiesterase 4 (PDE4) inhibitor Roflumilast N-oxide (RNO), alters RSV infection of well-differentiated HBE (WD-HBE) in vitro. WD-HBE were RSV infected in the presence or absence of RNO (0.1-100 nM). Viral infection (staining of F and G proteins, nucleoprotein RNA level), mRNA of ICAM-1, ciliated cell markers (digital high speed videomicroscopy, β-tubulin immunofluorescence, Foxj1 and Dnai2 mRNA), Goblet cells (PAS), mRNA of MUC5AC and CLCA1, mRNA and protein level of IL-13, IL-6, IL-8, TNFα, formation of H2O2 and the anti-oxidative armamentarium (mRNA of Nrf2, HO-1, GPx; total antioxidant capacity (TAC) were measured at day 10 or 15 post infection. RNO inhibited RSV infection of WD-HBE, prevented the loss of ciliated cells and markers, reduced the increase of MUC5AC and CLCA1 and inhibited the increase of IL-13, IL-6, IL-8, TNFα and ICAM-1. Additionally RNO reversed the reduction of Nrf2, HO-1 and GPx mRNA levels and consequently restored the TAC and reduced the H2O2 formation. RNO inhibits RSV infection of WD-HBE cultures and mitigates the cytopathological changes associated to this virus.
format	article
author	Manuel Mata Isidoro Martinez Jose A Melero Herman Tenor Julio Cortijo
author_facet	Manuel Mata Isidoro Martinez Jose A Melero Herman Tenor Julio Cortijo
author_sort	Manuel Mata
title	Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
title_short	Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
title_full	Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
title_fullStr	Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
title_full_unstemmed	Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
title_sort	roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/eeffae9a83494ce4973afe8664160734
work_keys_str_mv	AT manuelmata roflumilastinhibitsrespiratorysyncytialvirusinfectioninhumandifferentiatedbronchialepithelialcells AT isidoromartinez roflumilastinhibitsrespiratorysyncytialvirusinfectioninhumandifferentiatedbronchialepithelialcells AT joseamelero roflumilastinhibitsrespiratorysyncytialvirusinfectioninhumandifferentiatedbronchialepithelialcells AT hermantenor roflumilastinhibitsrespiratorysyncytialvirusinfectioninhumandifferentiatedbronchialepithelialcells AT juliocortijo roflumilastinhibitsrespiratorysyncytialvirusinfectioninhumandifferentiatedbronchialepithelialcells
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Roflumilast inhibits respiratory syncytial virus infection in human differentiated bronchial epithelial cells.

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