$Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation$
Código QR

Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation

Postbiotic fractions of several lactic acid bacteria have potential as microbial therapeutics for skin health and may also appeal to consumers who wish to avoid animal-based products. We aim to establish the optimum plant-peptone fermentation of Streptococcus macedonicus MBF10-2, which possess Bacte...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amira Aulia Musnadi, Amarila Malik, Amalia Sitti Khayyira
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	antibacterial antioxidant lysate soy peptone streptococcus macedonicus mbf 10-2 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/ef00602f73f04719a38f392352e72e9f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ef00602f73f04719a38f392352e72e9f
record_format	dspace
spelling	oai:doaj.org-article:ef00602f73f04719a38f392352e72e9f2021-11-15T06:08:47ZBioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation2338-94272338-948610.22146/ijp.1054https://doaj.org/article/ef00602f73f04719a38f392352e72e9f2021-02-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/1054https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Postbiotic fractions of several lactic acid bacteria have potential as microbial therapeutics for skin health and may also appeal to consumers who wish to avoid animal-based products. We aim to establish the optimum plant-peptone fermentation of Streptococcus macedonicus MBF10-2, which possess Bacteriocin Like-Inhibitory Substance activity in our previous study, to produce bacterial bioactive fractions. We evaluate their potential antibacterial and antioxidant actions, and as well assess the preliminary safety for human skin application. Fermentation was carried out by using plant peptone modified MRS, i.e., soy peptone and Vegitone, a non-animal-carbon sources that substitute proteose peptone in MRS medium. Fractions of MBF10-2 lysate and cell-free supernatant were collected and processed as follows, i.e. cell disruption, fraction separation and fractions freeze-drying. Fractions were confirm for antibacterial properties by the agar well diffusion method and assess for antioxidant activity using DPPH, while safety assessment was carried-out by skin patch assay. Maximum growth of MBF10-2 achieved by fermentation in soy peptone- and in Vegitone-modified media was 9.00 and 7.99 g total cell mass, respectively. The antibacterial property of fractions was most effective against Micrococcus luteus T18. The lysate fraction exhibited a mild antioxidant potency (IC50 840 µg/mL), and all bioactive fractions were proven safe and non-allergenic for human skins. Strep. macedonicus MBF10-2 postbiotics bioactive fractions were indicated as being safe for topical application. This is the first report on the production of a safe Strep. macedonicus bioactive postbiotic possessing mild antibacterial and mild-to-weak antioxidant.Amira Aulia MusnadiAmarila MalikAmalia Sitti KhayyiraUniversitas Gadjah Madaarticleantibacterialantioxidantlysatesoy peptonestreptococcus macedonicus mbf 10-2Pharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 52-63 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial antioxidant lysate soy peptone streptococcus macedonicus mbf 10-2 Pharmacy and materia medica RS1-441
spellingShingle	antibacterial antioxidant lysate soy peptone streptococcus macedonicus mbf 10-2 Pharmacy and materia medica RS1-441 Amira Aulia Musnadi Amarila Malik Amalia Sitti Khayyira Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation
description	Postbiotic fractions of several lactic acid bacteria have potential as microbial therapeutics for skin health and may also appeal to consumers who wish to avoid animal-based products. We aim to establish the optimum plant-peptone fermentation of Streptococcus macedonicus MBF10-2, which possess Bacteriocin Like-Inhibitory Substance activity in our previous study, to produce bacterial bioactive fractions. We evaluate their potential antibacterial and antioxidant actions, and as well assess the preliminary safety for human skin application. Fermentation was carried out by using plant peptone modified MRS, i.e., soy peptone and Vegitone, a non-animal-carbon sources that substitute proteose peptone in MRS medium. Fractions of MBF10-2 lysate and cell-free supernatant were collected and processed as follows, i.e. cell disruption, fraction separation and fractions freeze-drying. Fractions were confirm for antibacterial properties by the agar well diffusion method and assess for antioxidant activity using DPPH, while safety assessment was carried-out by skin patch assay. Maximum growth of MBF10-2 achieved by fermentation in soy peptone- and in Vegitone-modified media was 9.00 and 7.99 g total cell mass, respectively. The antibacterial property of fractions was most effective against Micrococcus luteus T18. The lysate fraction exhibited a mild antioxidant potency (IC50 840 µg/mL), and all bioactive fractions were proven safe and non-allergenic for human skins. Strep. macedonicus MBF10-2 postbiotics bioactive fractions were indicated as being safe for topical application. This is the first report on the production of a safe Strep. macedonicus bioactive postbiotic possessing mild antibacterial and mild-to-weak antioxidant.
format	article
author	Amira Aulia Musnadi Amarila Malik Amalia Sitti Khayyira
author_facet	Amira Aulia Musnadi Amarila Malik Amalia Sitti Khayyira
author_sort	Amira Aulia Musnadi
title	Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation
title_short	Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation
title_full	Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation
title_fullStr	Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation
title_full_unstemmed	Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation
title_sort	bioactive fractions from streptococcus macedonicus mbf 10-2 produced in an optimized plant-based peptone medium fermentation
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/ef00602f73f04719a38f392352e72e9f
work_keys_str_mv	AT amiraauliamusnadi bioactivefractionsfromstreptococcusmacedonicusmbf102producedinanoptimizedplantbasedpeptonemediumfermentation AT amarilamalik bioactivefractionsfromstreptococcusmacedonicusmbf102producedinanoptimizedplantbasedpeptonemediumfermentation AT amaliasittikhayyira bioactivefractionsfromstreptococcusmacedonicusmbf102producedinanoptimizedplantbasedpeptonemediumfermentation
_version_	1718428591380758528

Bioactive fractions from Streptococcus macedonicus MBF 10-2 produced in an optimized plant-based peptone medium fermentation

Ejemplares similares