Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China

Abstract To evaluate the diagnostic utilities of different methods for Mycobacterium tuberculosis (M. tuberculosis) detection in pleural fluid that represent potentially cost-effective measures for resource-limited settings in China. We compared diagnostic characteristics of the AmpSure simultaneous...

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Autores principales:	Qing Zhang, Caicun Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef227e230e4b4ed6a2b39a7eb1b1bc79
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spelling	oai:doaj.org-article:ef227e230e4b4ed6a2b39a7eb1b1bc792021-12-02T15:06:25ZComparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China10.1038/s41598-017-04872-62045-2322https://doaj.org/article/ef227e230e4b4ed6a2b39a7eb1b1bc792017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04872-6https://doaj.org/toc/2045-2322Abstract To evaluate the diagnostic utilities of different methods for Mycobacterium tuberculosis (M. tuberculosis) detection in pleural fluid that represent potentially cost-effective measures for resource-limited settings in China. We compared diagnostic characteristics of the AmpSure simultaneous amplification and testing method, the BACTEC MGIT 960 system, and acid-fast bacilli staining of specimen smears for detection of M. tuberculosis in pleural fluids of 438 patients with suspected tuberculous pleurisy. Chest radiographs, computed tomography scans and the results of sputum and pleural biopsy testing were used for confirmations of tuberculosis diagnoses. The sensitivity of the AmpSure test (21.2%) was significantly higher than that of smear analysis (3.1%; p < 0.001), but was similar to that of the BACTEC culture method (17.8%; p > 0.05). The specificity of the AmpSure, BACTEC, and smear tests were 100%, 96.4%, and 100%, respectively. The positive and negative predictive values of the AmpSure, BACTEC, and smear tests were 100%/23.1%; 78.6%/19.8%; and 100%/22.4%, respectively. The sensitivity of ADA, IFNγ and histopathological analyses of pleural biopsies were all 100%. The sensitivities of all three methods were suboptimal for the detection of M. tuberculosis in pleural fluid. Future studies of a composite diagnostic index consisting of a combination of these tests are warranted.Qing ZhangCaicun ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qing Zhang Caicun Zhou Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China
description	Abstract To evaluate the diagnostic utilities of different methods for Mycobacterium tuberculosis (M. tuberculosis) detection in pleural fluid that represent potentially cost-effective measures for resource-limited settings in China. We compared diagnostic characteristics of the AmpSure simultaneous amplification and testing method, the BACTEC MGIT 960 system, and acid-fast bacilli staining of specimen smears for detection of M. tuberculosis in pleural fluids of 438 patients with suspected tuberculous pleurisy. Chest radiographs, computed tomography scans and the results of sputum and pleural biopsy testing were used for confirmations of tuberculosis diagnoses. The sensitivity of the AmpSure test (21.2%) was significantly higher than that of smear analysis (3.1%; p < 0.001), but was similar to that of the BACTEC culture method (17.8%; p > 0.05). The specificity of the AmpSure, BACTEC, and smear tests were 100%, 96.4%, and 100%, respectively. The positive and negative predictive values of the AmpSure, BACTEC, and smear tests were 100%/23.1%; 78.6%/19.8%; and 100%/22.4%, respectively. The sensitivity of ADA, IFNγ and histopathological analyses of pleural biopsies were all 100%. The sensitivities of all three methods were suboptimal for the detection of M. tuberculosis in pleural fluid. Future studies of a composite diagnostic index consisting of a combination of these tests are warranted.
format	article
author	Qing Zhang Caicun Zhou
author_facet	Qing Zhang Caicun Zhou
author_sort	Qing Zhang
title	Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China
title_short	Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China
title_full	Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China
title_fullStr	Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China
title_full_unstemmed	Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China
title_sort	comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in china
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef227e230e4b4ed6a2b39a7eb1b1bc79
work_keys_str_mv	AT qingzhang comparisonoflaboratorytestingmethodsforthediagnosisoftuberculouspleurisyinchina AT caicunzhou comparisonoflaboratorytestingmethodsforthediagnosisoftuberculouspleurisyinchina
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Comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in China

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