Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor

Abstract Droplets microfluidics is broadening the range of Lab on a Chip solutions that, however, still suffer from the lack of an adequate level of integration of optical detection and sensors. In fact, droplets are currently monitored by imaging techniques, mostly limited by a time-consuming data...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	R. Zamboni, A. Zaltron, M. Chauvet, C. Sada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef267e338b184ee9842c15e3d7f760f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ef267e338b184ee9842c15e3d7f760f6
record_format	dspace
spelling	oai:doaj.org-article:ef267e338b184ee9842c15e3d7f760f62021-12-02T17:19:16ZReal-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor10.1038/s41598-021-97392-32045-2322https://doaj.org/article/ef267e338b184ee9842c15e3d7f760f62021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97392-3https://doaj.org/toc/2045-2322Abstract Droplets microfluidics is broadening the range of Lab on a Chip solutions that, however, still suffer from the lack of an adequate level of integration of optical detection and sensors. In fact, droplets are currently monitored by imaging techniques, mostly limited by a time-consuming data post-processing and big data storage. This work aims to overcome this weakness, presenting a fully integrated opto-microfluidic platform able to detect, label and characterize droplets without the need for imaging techniques. It consists of optical waveguides arranged in a Mach Zehnder’s configuration and a microfluidic circuit both coupled in the same substrate. As a proof of concept, the work demonstrates the performances of this opto-microfluidic platform in performing a complete and simultaneous sequence labelling and identification of each single droplet, in terms of its optical properties, as well as velocity and lengths. Since the sensor is realized in lithium niobate crystals, which is also highly resistant to chemical attack and biocompatible, the future addition of multifunctional stages into the same substrate can be easily envisioned, extending the range of applicability of the final device.R. ZamboniA. ZaltronM. ChauvetC. SadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q R. Zamboni A. Zaltron M. Chauvet C. Sada Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
description	Abstract Droplets microfluidics is broadening the range of Lab on a Chip solutions that, however, still suffer from the lack of an adequate level of integration of optical detection and sensors. In fact, droplets are currently monitored by imaging techniques, mostly limited by a time-consuming data post-processing and big data storage. This work aims to overcome this weakness, presenting a fully integrated opto-microfluidic platform able to detect, label and characterize droplets without the need for imaging techniques. It consists of optical waveguides arranged in a Mach Zehnder’s configuration and a microfluidic circuit both coupled in the same substrate. As a proof of concept, the work demonstrates the performances of this opto-microfluidic platform in performing a complete and simultaneous sequence labelling and identification of each single droplet, in terms of its optical properties, as well as velocity and lengths. Since the sensor is realized in lithium niobate crystals, which is also highly resistant to chemical attack and biocompatible, the future addition of multifunctional stages into the same substrate can be easily envisioned, extending the range of applicability of the final device.
format	article
author	R. Zamboni A. Zaltron M. Chauvet C. Sada
author_facet	R. Zamboni A. Zaltron M. Chauvet C. Sada
author_sort	R. Zamboni
title	Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
title_short	Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
title_full	Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
title_fullStr	Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
title_full_unstemmed	Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
title_sort	real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ef267e338b184ee9842c15e3d7f760f6
work_keys_str_mv	AT rzamboni realtimeprecisemicrofluidicdropletslabelsequencingcombinedinavelocitydetectionsensor AT azaltron realtimeprecisemicrofluidicdropletslabelsequencingcombinedinavelocitydetectionsensor AT mchauvet realtimeprecisemicrofluidicdropletslabelsequencingcombinedinavelocitydetectionsensor AT csada realtimeprecisemicrofluidicdropletslabelsequencingcombinedinavelocitydetectionsensor
_version_	1718381058909536256

Real-time precise microfluidic droplets label-sequencing combined in a velocity detection sensor

Ejemplares similares