Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells

Abstract Magnoliae Flos (MF) is a traditional medicinal herb used for managing rhinitis, sinusitis and headache. The purpose of the present study was to determine the neuroprotective effect of MF against glutamate-induced oxidative stress and to assess the underlying mechanism. Glutamate is a major...

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Autores principales:	Youn Sik Jung, Jin Bae Weon, Woo Seung Yang, Gahee Ryu, Choong Je Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef29e4698fb64de891ee06b7d3888a03
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spelling	oai:doaj.org-article:ef29e4698fb64de891ee06b7d3888a032021-12-02T15:07:47ZNeuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells10.1038/s41598-018-28055-z2045-2322https://doaj.org/article/ef29e4698fb64de891ee06b7d3888a032018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-28055-zhttps://doaj.org/toc/2045-2322Abstract Magnoliae Flos (MF) is a traditional medicinal herb used for managing rhinitis, sinusitis and headache. The purpose of the present study was to determine the neuroprotective effect of MF against glutamate-induced oxidative stress and to assess the underlying mechanism. Glutamate is a major endogenous excitatory neurotransmitter in the brain and contributes to the development of neurodegenerative diseases by excessive activation. MF extract was subjected to a neuroprotective effect assay in HT22 mouse hippocampal cells. The mechanism underlying the neuroprotective effect of MF extract was evaluated by assaying reactive oxygen species (ROS) levels, intracellular Ca2+ levels, mitochondrial membrane potential, glutathione level and antioxidant enzyme activity in HT22 cells. MF extract significantly decreased glutamate-induced death of HT22 cells (80.83 ± 7.34% relative neuroprotection). MF extract reduced the intracellular ROS and Ca2+ levels and increased the glutathione level and glutathione reductase and glutathione peroxide activities. Moreover, MF extract attenuated the mitochondrial membrane potential in HT22 cells. These results suggested that MF extract exerts a neuroprotective effect against oxidative stress HT22 cells, which was mediated by its antioxidant activity.Youn Sik JungJin Bae WeonWoo Seung YangGahee RyuChoong Je MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Youn Sik Jung Jin Bae Weon Woo Seung Yang Gahee Ryu Choong Je Ma Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells
description	Abstract Magnoliae Flos (MF) is a traditional medicinal herb used for managing rhinitis, sinusitis and headache. The purpose of the present study was to determine the neuroprotective effect of MF against glutamate-induced oxidative stress and to assess the underlying mechanism. Glutamate is a major endogenous excitatory neurotransmitter in the brain and contributes to the development of neurodegenerative diseases by excessive activation. MF extract was subjected to a neuroprotective effect assay in HT22 mouse hippocampal cells. The mechanism underlying the neuroprotective effect of MF extract was evaluated by assaying reactive oxygen species (ROS) levels, intracellular Ca2+ levels, mitochondrial membrane potential, glutathione level and antioxidant enzyme activity in HT22 cells. MF extract significantly decreased glutamate-induced death of HT22 cells (80.83 ± 7.34% relative neuroprotection). MF extract reduced the intracellular ROS and Ca2+ levels and increased the glutathione level and glutathione reductase and glutathione peroxide activities. Moreover, MF extract attenuated the mitochondrial membrane potential in HT22 cells. These results suggested that MF extract exerts a neuroprotective effect against oxidative stress HT22 cells, which was mediated by its antioxidant activity.
format	article
author	Youn Sik Jung Jin Bae Weon Woo Seung Yang Gahee Ryu Choong Je Ma
author_facet	Youn Sik Jung Jin Bae Weon Woo Seung Yang Gahee Ryu Choong Je Ma
author_sort	Youn Sik Jung
title	Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells
title_short	Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells
title_full	Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells
title_fullStr	Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells
title_full_unstemmed	Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells
title_sort	neuroprotective effects of magnoliae flos extract in mouse hippocampal neuronal cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ef29e4698fb64de891ee06b7d3888a03
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_version_	1718388423357628416

Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells

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