Tunable uptake/release mechanism of protein microgel particles in biomimicking environment

Abstract Microgels are intra-molecular crosslinked macromolecules that can be used as vehicles to deliver and release drugs at the point-of-need in the patient’s body. Here, gelatin microgels were formed from microfluidics droplets, stabilised by aldehydes and frozen into a spheroidal shape. Microge...

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Autores principales:	A. Pepe, P. Podesva, G. Simone
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef2a5789afca41f58de8bb6a2b342c27
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spelling	oai:doaj.org-article:ef2a5789afca41f58de8bb6a2b342c272021-12-02T15:04:52ZTunable uptake/release mechanism of protein microgel particles in biomimicking environment10.1038/s41598-017-06512-52045-2322https://doaj.org/article/ef2a5789afca41f58de8bb6a2b342c272017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06512-5https://doaj.org/toc/2045-2322Abstract Microgels are intra-molecular crosslinked macromolecules that can be used as vehicles to deliver and release drugs at the point-of-need in the patient’s body. Here, gelatin microgels were formed from microfluidics droplets, stabilised by aldehydes and frozen into a spheroidal shape. Microgel morphology and response to external stimuli were characterised. It was found that the behaviour of the spheroidal microgels was sensitive to both pH and ionic strength and that the distribution of charges into the microgels affected the behaviour of swelling and uptake. The uptake of molecules such as Rhodamine B and Methylene Blue were investigated as a model for drug uptake/release mechanisms. Under physiological conditions, the uptake of Rhodamine was rapid and a uniform distribution of the fluorescent molecules was recorded inside the microgels. However, the mechanism of release became slower at lower pH, which mimics the stomach environment. Under physiological conditions, Methylene Blue release occurred faster than for Rhodamine. Anionic and neutral molecules were also tested. In conclusion, the dependence of uptake and release of model drugs on basic/acid conditions shows that microgels could be used for targeted drug delivery. Different shaped microgels, such as spheres, spheroids, and rods, could be useful in tissue engineering or during vascularisation.A. PepeP. PodesvaG. SimoneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Pepe P. Podesva G. Simone Tunable uptake/release mechanism of protein microgel particles in biomimicking environment
description	Abstract Microgels are intra-molecular crosslinked macromolecules that can be used as vehicles to deliver and release drugs at the point-of-need in the patient’s body. Here, gelatin microgels were formed from microfluidics droplets, stabilised by aldehydes and frozen into a spheroidal shape. Microgel morphology and response to external stimuli were characterised. It was found that the behaviour of the spheroidal microgels was sensitive to both pH and ionic strength and that the distribution of charges into the microgels affected the behaviour of swelling and uptake. The uptake of molecules such as Rhodamine B and Methylene Blue were investigated as a model for drug uptake/release mechanisms. Under physiological conditions, the uptake of Rhodamine was rapid and a uniform distribution of the fluorescent molecules was recorded inside the microgels. However, the mechanism of release became slower at lower pH, which mimics the stomach environment. Under physiological conditions, Methylene Blue release occurred faster than for Rhodamine. Anionic and neutral molecules were also tested. In conclusion, the dependence of uptake and release of model drugs on basic/acid conditions shows that microgels could be used for targeted drug delivery. Different shaped microgels, such as spheres, spheroids, and rods, could be useful in tissue engineering or during vascularisation.
format	article
author	A. Pepe P. Podesva G. Simone
author_facet	A. Pepe P. Podesva G. Simone
author_sort	A. Pepe
title	Tunable uptake/release mechanism of protein microgel particles in biomimicking environment
title_short	Tunable uptake/release mechanism of protein microgel particles in biomimicking environment
title_full	Tunable uptake/release mechanism of protein microgel particles in biomimicking environment
title_fullStr	Tunable uptake/release mechanism of protein microgel particles in biomimicking environment
title_full_unstemmed	Tunable uptake/release mechanism of protein microgel particles in biomimicking environment
title_sort	tunable uptake/release mechanism of protein microgel particles in biomimicking environment
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef2a5789afca41f58de8bb6a2b342c27
work_keys_str_mv	AT apepe tunableuptakereleasemechanismofproteinmicrogelparticlesinbiomimickingenvironment AT ppodesva tunableuptakereleasemechanismofproteinmicrogelparticlesinbiomimickingenvironment AT gsimone tunableuptakereleasemechanismofproteinmicrogelparticlesinbiomimickingenvironment
_version_	1718389052499034112

Tunable uptake/release mechanism of protein microgel particles in biomimicking environment

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