Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape

Abstract The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Martin Stortz, Diego M. Presman, Luciana Bruno, Paolo Annibale, Maria V. Dansey, Gerardo Burton, Enrico Gratton, Adali Pecci, Valeria Levi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef356766e870405287f98d124f438e45
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ef356766e870405287f98d124f438e45
record_format	dspace
spelling	oai:doaj.org-article:ef356766e870405287f98d124f438e452021-12-02T15:05:55ZMapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape10.1038/s41598-017-06676-02045-2322https://doaj.org/article/ef356766e870405287f98d124f438e452017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06676-0https://doaj.org/toc/2045-2322Abstract The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorticoid receptor (GR) in the interphase nucleus of living cells. We found that this ligand-activated transcription factor diffuses within the nucleus and dynamically interacts with bodies enriched in the coregulator NCoA-2, DNA-dependent foci and chromatin targets. The distribution of the receptor among the nuclear compartments depends on NCoA-2 and the conformation of the receptor as assessed with synthetic ligands and GR mutants with impaired transcriptional abilities. Our results suggest that the partition of the receptor in different nuclear reservoirs ultimately regulates the concentration of receptor available for the interaction with specific targets, and thus has an impact on transcription regulation.Martin StortzDiego M. PresmanLuciana BrunoPaolo AnnibaleMaria V. DanseyGerardo BurtonEnrico GrattonAdali PecciValeria LeviNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Stortz Diego M. Presman Luciana Bruno Paolo Annibale Maria V. Dansey Gerardo Burton Enrico Gratton Adali Pecci Valeria Levi Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
description	Abstract The distribution of the transcription machinery among different sub-nuclear domains raises the question on how the architecture of the nucleus modulates the transcriptional response. Here, we used fluorescence fluctuation analyses to quantitatively explore the organization of the glucocorticoid receptor (GR) in the interphase nucleus of living cells. We found that this ligand-activated transcription factor diffuses within the nucleus and dynamically interacts with bodies enriched in the coregulator NCoA-2, DNA-dependent foci and chromatin targets. The distribution of the receptor among the nuclear compartments depends on NCoA-2 and the conformation of the receptor as assessed with synthetic ligands and GR mutants with impaired transcriptional abilities. Our results suggest that the partition of the receptor in different nuclear reservoirs ultimately regulates the concentration of receptor available for the interaction with specific targets, and thus has an impact on transcription regulation.
format	article
author	Martin Stortz Diego M. Presman Luciana Bruno Paolo Annibale Maria V. Dansey Gerardo Burton Enrico Gratton Adali Pecci Valeria Levi
author_facet	Martin Stortz Diego M. Presman Luciana Bruno Paolo Annibale Maria V. Dansey Gerardo Burton Enrico Gratton Adali Pecci Valeria Levi
author_sort	Martin Stortz
title	Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_short	Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_full	Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_fullStr	Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_full_unstemmed	Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape
title_sort	mapping the dynamics of the glucocorticoid receptor within the nuclear landscape
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef356766e870405287f98d124f438e45
work_keys_str_mv	AT martinstortz mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT diegompresman mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT lucianabruno mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT paoloannibale mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT mariavdansey mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT gerardoburton mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT enricogratton mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT adalipecci mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape AT valerialevi mappingthedynamicsoftheglucocorticoidreceptorwithinthenuclearlandscape
_version_	1718388679404158976

Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape

Ejemplares similares