Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells

Abstract Background The lncRNA H19 is believed to act as an oncogene in various types of tumors and is considered to be a therapeutic target and diagnostic marker. However, the role of the lncRNA H19 in regulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells is unknown. Methods T...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xueshan Zhao, Xiaodong Jin, Qiuning Zhang, Ruifeng Liu, Hongtao Luo, Zhen Yang, Yichao Geng, Shuangwu Feng, Chengcheng Li, Lina Wang, Xiaohu Wang, Qiang Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	LncRNA H19 MiR-130a-3p WNK3 Non-small-cell lung cancer Radiotherapy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/ef391dba8d104e748a3b48991a30c30e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ef391dba8d104e748a3b48991a30c30e
record_format	dspace
spelling	oai:doaj.org-article:ef391dba8d104e748a3b48991a30c30e2021-12-05T12:23:45ZSilencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells10.1186/s12935-021-02268-11475-2867https://doaj.org/article/ef391dba8d104e748a3b48991a30c30e2021-12-01T00:00:00Zhttps://doi.org/10.1186/s12935-021-02268-1https://doaj.org/toc/1475-2867Abstract Background The lncRNA H19 is believed to act as an oncogene in various types of tumors and is considered to be a therapeutic target and diagnostic marker. However, the role of the lncRNA H19 in regulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells is unknown. Methods The expression profiles of lncRNAs in NSCLC were explored via transcriptome sequencing. CCK-8, EdU incorporation and clonogenic survival assays were conducted to evaluate the proliferation and radiosensitivity of NSCLC cells. Flow cytometry and Western blotting were conducted to measure the level of apoptosis. The binding relationship between the lncRNA H19 and miR-130a-3p was determined by a dual-luciferase reporter assay. A binding relationship was also identified between miR-130a-3p and With-No-Lysine Kinase 3 (WNK3). Results Expression patterns of lncRNAs revealed that the lncRNA H19 was upregulated in radioresistant NSCLC (A549-R11) cells compared with A549 cells. Knockdown of the lncRNA H19 enhanced the sensitivity of NSCLC cell lines to X-ray and carbon ion irradiation. Mechanistically, the lncRNA H19 serves as a sponge of miR-130a-3p, which downregulates WNK3 expression. The lncRNA H19–miR-130a-3p–WNK3 axis modulates radiosensitivity by regulating apoptosis in NSCLC cell lines. Conclusion Knockdown of the lncRNA H19 promotes the sensitivity of NSCLC cells to X-ray and carbon ion irradiation. Hence, the lncRNA H19 might function as a potential therapeutic target that enhances the antitumor effects of radiotherapy in NSCLC.Xueshan ZhaoXiaodong JinQiuning ZhangRuifeng LiuHongtao LuoZhen YangYichao GengShuangwu FengChengcheng LiLina WangXiaohu WangQiang LiBMCarticleLncRNA H19MiR-130a-3pWNK3Non-small-cell lung cancerRadiotherapyNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCancer Cell International, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	LncRNA H19 MiR-130a-3p WNK3 Non-small-cell lung cancer Radiotherapy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671
spellingShingle	LncRNA H19 MiR-130a-3p WNK3 Non-small-cell lung cancer Radiotherapy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Xueshan Zhao Xiaodong Jin Qiuning Zhang Ruifeng Liu Hongtao Luo Zhen Yang Yichao Geng Shuangwu Feng Chengcheng Li Lina Wang Xiaohu Wang Qiang Li Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
description	Abstract Background The lncRNA H19 is believed to act as an oncogene in various types of tumors and is considered to be a therapeutic target and diagnostic marker. However, the role of the lncRNA H19 in regulating the radiosensitivity of non-small cell lung cancer (NSCLC) cells is unknown. Methods The expression profiles of lncRNAs in NSCLC were explored via transcriptome sequencing. CCK-8, EdU incorporation and clonogenic survival assays were conducted to evaluate the proliferation and radiosensitivity of NSCLC cells. Flow cytometry and Western blotting were conducted to measure the level of apoptosis. The binding relationship between the lncRNA H19 and miR-130a-3p was determined by a dual-luciferase reporter assay. A binding relationship was also identified between miR-130a-3p and With-No-Lysine Kinase 3 (WNK3). Results Expression patterns of lncRNAs revealed that the lncRNA H19 was upregulated in radioresistant NSCLC (A549-R11) cells compared with A549 cells. Knockdown of the lncRNA H19 enhanced the sensitivity of NSCLC cell lines to X-ray and carbon ion irradiation. Mechanistically, the lncRNA H19 serves as a sponge of miR-130a-3p, which downregulates WNK3 expression. The lncRNA H19–miR-130a-3p–WNK3 axis modulates radiosensitivity by regulating apoptosis in NSCLC cell lines. Conclusion Knockdown of the lncRNA H19 promotes the sensitivity of NSCLC cells to X-ray and carbon ion irradiation. Hence, the lncRNA H19 might function as a potential therapeutic target that enhances the antitumor effects of radiotherapy in NSCLC.
format	article
author	Xueshan Zhao Xiaodong Jin Qiuning Zhang Ruifeng Liu Hongtao Luo Zhen Yang Yichao Geng Shuangwu Feng Chengcheng Li Lina Wang Xiaohu Wang Qiang Li
author_facet	Xueshan Zhao Xiaodong Jin Qiuning Zhang Ruifeng Liu Hongtao Luo Zhen Yang Yichao Geng Shuangwu Feng Chengcheng Li Lina Wang Xiaohu Wang Qiang Li
author_sort	Xueshan Zhao
title	Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
title_short	Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
title_full	Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
title_fullStr	Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
title_full_unstemmed	Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells
title_sort	silencing of the lncrna h19 enhances sensitivity to x-ray and carbon-ions through the mir-130a-3p /wnk3 signaling axis in nsclc cells
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ef391dba8d104e748a3b48991a30c30e
work_keys_str_mv	AT xueshanzhao silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT xiaodongjin silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT qiuningzhang silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT ruifengliu silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT hongtaoluo silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT zhenyang silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT yichaogeng silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT shuangwufeng silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT chengchengli silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT linawang silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT xiaohuwang silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells AT qiangli silencingofthelncrnah19enhancessensitivitytoxrayandcarbonionsthroughthemir130a3pwnk3signalingaxisinnsclccells
_version_	1718371993336676352

Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells

Ejemplares similares