A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers
Abstract The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by...
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Nature Portfolio
2021
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oai:doaj.org-article:ef43827c652c4f9188991f1e481385de2021-12-02T15:53:45ZA centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers10.1038/s41598-021-84353-z2045-2322https://doaj.org/article/ef43827c652c4f9188991f1e481385de2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84353-zhttps://doaj.org/toc/2045-2322Abstract The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need to separate hydrophobic signatures such as lipids from blood. Herein, we present a microfluidic device capable of centrifugal separation of serum from blood at the point of need with a system that is compatible with biomarkers that are both hydrophilic and hydrophobic. The cross-flow filtration device separates serum from blood as efficiently as traditional methods and retains amphiphilic biomarkers in serum for detection.Kiersten D. LenzShailja JakharJing W. ChenAaron S. AndersonDylan C. PurcellMohammad O. IshakJennifer F. HarrisLeyla E. AkhadovJessica Z. Kubicek-SutherlandPulak NathHarshini MukundanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Kiersten D. Lenz Shailja Jakhar Jing W. Chen Aaron S. Anderson Dylan C. Purcell Mohammad O. Ishak Jennifer F. Harris Leyla E. Akhadov Jessica Z. Kubicek-Sutherland Pulak Nath Harshini Mukundan A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| description |
Abstract The separation of biomarkers from blood is straightforward in most molecular biology laboratories. However, separation in resource-limited settings, allowing for the successful removal of biomarkers for diagnostic applications, is not always possible. The situation is further complicated by the need to separate hydrophobic signatures such as lipids from blood. Herein, we present a microfluidic device capable of centrifugal separation of serum from blood at the point of need with a system that is compatible with biomarkers that are both hydrophilic and hydrophobic. The cross-flow filtration device separates serum from blood as efficiently as traditional methods and retains amphiphilic biomarkers in serum for detection. |
| format |
article |
| author |
Kiersten D. Lenz Shailja Jakhar Jing W. Chen Aaron S. Anderson Dylan C. Purcell Mohammad O. Ishak Jennifer F. Harris Leyla E. Akhadov Jessica Z. Kubicek-Sutherland Pulak Nath Harshini Mukundan |
| author_facet |
Kiersten D. Lenz Shailja Jakhar Jing W. Chen Aaron S. Anderson Dylan C. Purcell Mohammad O. Ishak Jennifer F. Harris Leyla E. Akhadov Jessica Z. Kubicek-Sutherland Pulak Nath Harshini Mukundan |
| author_sort |
Kiersten D. Lenz |
| title |
A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| title_short |
A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| title_full |
A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| title_fullStr |
A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| title_full_unstemmed |
A centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| title_sort |
centrifugal microfluidic cross-flow filtration platform to separate serum from whole blood for the detection of amphiphilic biomarkers |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/ef43827c652c4f9188991f1e481385de |
| work_keys_str_mv |
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