Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy

Gerald G Enriquez,1 Syed AA Rizvi,2 Martin J D’Souza,3 Duc P Do1 1Department of Pharmaceutical Sciences, College of Pharmacy, Chicago State University, Chicago, IL, 2Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL, 3Department o...

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Autores principales:	Enriquez GG, Rizvi SAA, D&rsquo;Souza MJ, Do DP
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ef47df7ff2874f0b9b3b03a7e7707873
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spelling	oai:doaj.org-article:ef47df7ff2874f0b9b3b03a7e77078732021-12-02T05:02:09ZFormulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy1176-91141178-2013https://doaj.org/article/ef47df7ff2874f0b9b3b03a7e77078732013-04-01T00:00:00Zhttp://www.dovepress.com/formulation-and-evaluation-of-drug-loaded-targeted-magnetic-microspher-a12713https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Gerald G Enriquez,1 Syed AA Rizvi,2 Martin J D’Souza,3 Duc P Do1 1Department of Pharmaceutical Sciences, College of Pharmacy, Chicago State University, Chicago, IL, 2Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL, 3Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Mercer University, Atlanta, GA, USA Abstract: Enhanced and targeted drug delivery using biodegradable microspheres is emerging as a promising approach for cancer therapy. The main objective of the present research was to formulate, characterize, and evaluate iron oxide (magnetic) containing a bovine serum albumin-based microsphere drug delivery system, capable of efficiently delivering sulforaphane, a histone deacetylase inhibitor, for an extended period of time in vivo. Magnetic microspheres were prepared by spray-drying and characterized for their physicochemical properties and dissolution profile. Further, they were evaluated for therapeutic efficacy in in vitro and in vivo systems. In vitro studies in B16 melanoma cells revealed that there was about 13%–16% more inhibition of cell viability when either 30 µM or 50 µM of sulforaphane was used with iron oxide in the polymeric carrier. Data from in vivo studies in C57BL/6 mice revealed that the magnetic microspheres (localized to the tumor site with the help of a strong magnet) inhibited 18% more tumor growth as compared with sulforaphane in solution. In addition, there was a 40% reduction in histone deacetylation levels in mice treated with iron oxide microspheres containing sulforaphane. Thus, magnetic microspheres are shown to be an effective drug delivery system for anticancer drugs. Keywords: sulforaphane, delivery system, epigenetic therapy, microspheres, histone deacetylase inhibitor, iron oxideEnriquez GGRizvi SAAD&rsquo;Souza MJDo DPDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1393-1402 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Enriquez GG Rizvi SAA D&rsquo;Souza MJ Do DP Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
description	Gerald G Enriquez,1 Syed AA Rizvi,2 Martin J D’Souza,3 Duc P Do1 1Department of Pharmaceutical Sciences, College of Pharmacy, Chicago State University, Chicago, IL, 2Department of Pharmaceutical Sciences, College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL, 3Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Mercer University, Atlanta, GA, USA Abstract: Enhanced and targeted drug delivery using biodegradable microspheres is emerging as a promising approach for cancer therapy. The main objective of the present research was to formulate, characterize, and evaluate iron oxide (magnetic) containing a bovine serum albumin-based microsphere drug delivery system, capable of efficiently delivering sulforaphane, a histone deacetylase inhibitor, for an extended period of time in vivo. Magnetic microspheres were prepared by spray-drying and characterized for their physicochemical properties and dissolution profile. Further, they were evaluated for therapeutic efficacy in in vitro and in vivo systems. In vitro studies in B16 melanoma cells revealed that there was about 13%–16% more inhibition of cell viability when either 30 µM or 50 µM of sulforaphane was used with iron oxide in the polymeric carrier. Data from in vivo studies in C57BL/6 mice revealed that the magnetic microspheres (localized to the tumor site with the help of a strong magnet) inhibited 18% more tumor growth as compared with sulforaphane in solution. In addition, there was a 40% reduction in histone deacetylation levels in mice treated with iron oxide microspheres containing sulforaphane. Thus, magnetic microspheres are shown to be an effective drug delivery system for anticancer drugs. Keywords: sulforaphane, delivery system, epigenetic therapy, microspheres, histone deacetylase inhibitor, iron oxide
format	article
author	Enriquez GG Rizvi SAA D&rsquo;Souza MJ Do DP
author_facet	Enriquez GG Rizvi SAA D&rsquo;Souza MJ Do DP
author_sort	Enriquez GG
title	Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
title_short	Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
title_full	Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
title_fullStr	Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
title_full_unstemmed	Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
title_sort	formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/ef47df7ff2874f0b9b3b03a7e7707873
work_keys_str_mv	AT enriquezgg formulationandevaluationofdrugloadedtargetedmagneticmicrospheresforcancertherapy AT rizvisaa formulationandevaluationofdrugloadedtargetedmagneticmicrospheresforcancertherapy AT damprsquosouzamj formulationandevaluationofdrugloadedtargetedmagneticmicrospheresforcancertherapy AT dodp formulationandevaluationofdrugloadedtargetedmagneticmicrospheresforcancertherapy
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Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy

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