Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy

Abstract Methodology for accurate quantification of intra-hepatic cccDNA has long been a technical challenge, yet it is highly desired in the clinic. Here, we developed a sensitive method for quantification of intrahepatic cccDNA in liver biopsies from patients, which allowed to predict patient’s re...

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Autores principales:	Di Mu, Fang-Chao Yuan, Yu Chen, Xiao-Yan Jiang, Liang Yan, Ling-Yu Jiang, Jian-Ping Gong, Da-Zhi Zhang, Hong Ren, Yong Liao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef4cdf48b2f048d9a009d26616692177
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spelling	oai:doaj.org-article:ef4cdf48b2f048d9a009d266166921772021-12-02T16:08:11ZBaseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy10.1038/s41598-017-05242-y2045-2322https://doaj.org/article/ef4cdf48b2f048d9a009d266166921772017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05242-yhttps://doaj.org/toc/2045-2322Abstract Methodology for accurate quantification of intra-hepatic cccDNA has long been a technical challenge, yet it is highly desired in the clinic. Here, we developed a sensitive method for quantification of intrahepatic cccDNA in liver biopsies from patients, which allowed to predict patient’s response to interferon therapy at baseline. Twenty-five patients with HBeAg+ CHB were recruited and liver biopsies were obtained at baseline and 1-year after interferon treatment, respectively. Both intrahepatic cccDNA and HBV DNA were absolutely quantified by a droplet digital PCR amplification system. Patients were categorized as either responder or non-responder group based on their HBeAg status 1-year after interferon therapy. Levels of both intrahepatic HBV DNA and HBV cccDNA were significantly reduced after interferon treatment among the responders, but not the non-responders, in comparison with their levels at baseline. Baseline values of intrahepatic HBV DNA over cccDNA significantly correlated with patient’s response to PEG-IFN therapy (P = 0.000). In addition, HBeAg seroconversion also correlates with a significant reduction in intrahepatic pgRNA production among the responders after interferon therapy (P = 0.030). In conclusion, our results suggest that baseline value of intrahepatic HBV DNA over cccDNA may be a preferable indicator for selecting appropriate patients for IFN-based therapy in the clinic.Di MuFang-Chao YuanYu ChenXiao-Yan JiangLiang YanLing-Yu JiangJian-Ping GongDa-Zhi ZhangHong RenYong LiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Di Mu Fang-Chao Yuan Yu Chen Xiao-Yan Jiang Liang Yan Ling-Yu Jiang Jian-Ping Gong Da-Zhi Zhang Hong Ren Yong Liao Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy
description	Abstract Methodology for accurate quantification of intra-hepatic cccDNA has long been a technical challenge, yet it is highly desired in the clinic. Here, we developed a sensitive method for quantification of intrahepatic cccDNA in liver biopsies from patients, which allowed to predict patient’s response to interferon therapy at baseline. Twenty-five patients with HBeAg+ CHB were recruited and liver biopsies were obtained at baseline and 1-year after interferon treatment, respectively. Both intrahepatic cccDNA and HBV DNA were absolutely quantified by a droplet digital PCR amplification system. Patients were categorized as either responder or non-responder group based on their HBeAg status 1-year after interferon therapy. Levels of both intrahepatic HBV DNA and HBV cccDNA were significantly reduced after interferon treatment among the responders, but not the non-responders, in comparison with their levels at baseline. Baseline values of intrahepatic HBV DNA over cccDNA significantly correlated with patient’s response to PEG-IFN therapy (P = 0.000). In addition, HBeAg seroconversion also correlates with a significant reduction in intrahepatic pgRNA production among the responders after interferon therapy (P = 0.030). In conclusion, our results suggest that baseline value of intrahepatic HBV DNA over cccDNA may be a preferable indicator for selecting appropriate patients for IFN-based therapy in the clinic.
format	article
author	Di Mu Fang-Chao Yuan Yu Chen Xiao-Yan Jiang Liang Yan Ling-Yu Jiang Jian-Ping Gong Da-Zhi Zhang Hong Ren Yong Liao
author_facet	Di Mu Fang-Chao Yuan Yu Chen Xiao-Yan Jiang Liang Yan Ling-Yu Jiang Jian-Ping Gong Da-Zhi Zhang Hong Ren Yong Liao
author_sort	Di Mu
title	Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy
title_short	Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy
title_full	Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy
title_fullStr	Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy
title_full_unstemmed	Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy
title_sort	baseline value of intrahepatic hbv dna over cccdna predicts patient’s response to interferon therapy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef4cdf48b2f048d9a009d26616692177
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Baseline value of intrahepatic HBV DNA over cccDNA predicts patient’s response to interferon therapy

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