MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells

Classical swine fever virus (CSFV) is one of the most important viral pathogens leading worldwide threats to pig industry. MicroRNAs (miRNAs) play important roles in regulating virus replication, but whether miRNAs affect CSFV infection is still poorly understood. In previous study, we identified fo...

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Autores principales:	Panpan Xu, Shuangkai Jia, Kai Wang, Zhixin Fan, Hongqing Zheng, Jiangman Lv, Yanfen Jiang, Yufeng Hou, Bihao Lou, Hongchao Zhou, Yanming Zhang, Kangkang Guo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	classical swine fever virus mir-140 rab25 3ʹ utr inhibition Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ef501e4aca8841e1b79849b1c6b07bc3
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spelling	oai:doaj.org-article:ef501e4aca8841e1b79849b1c6b07bc32021-11-17T14:21:58ZMiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells2150-55942150-560810.1080/21505594.2020.1735051https://doaj.org/article/ef501e4aca8841e1b79849b1c6b07bc32020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1735051https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Classical swine fever virus (CSFV) is one of the most important viral pathogens leading worldwide threats to pig industry. MicroRNAs (miRNAs) play important roles in regulating virus replication, but whether miRNAs affect CSFV infection is still poorly understood. In previous study, we identified four miRNAs that were down-regulated by CSFV in swine umbilical vein endothelial cells (SUVEC). In this study, miR-140, one of the most potently down-regulated genes was investigated. We found that the miRNA expression was significantly inhibited by CSFV infection. Subsequent studies revealed that miR-140 mimics signiﬁcantly inhibited CSFV replication, while the inhibition of endogenous miR-140 enhanced CSFV replication. By using bioinformatics prediction, luciferase reporter system, real-time fluorescence quantitative PCR (RT-qPCR) and Western blot assays, we further demonstrated that miR-140 bind to the 3ʹ UTR of Rab25 mRNA to regulate its expression. We also analyzed the expression pattern of Rab25 in SUVECs after CSFV infection. The results showed that CSFV infection induced Rab25 expression. Finally, Rab25 was found to promote CSFV replication. In conclusion, this study demonstrated that CSFV inhibits miR-140 expression and miR-140 inhibits replication by binding to host factor Rab25.Panpan XuShuangkai JiaKai WangZhixin FanHongqing ZhengJiangman LvYanfen JiangYufeng HouBihao LouHongchao ZhouYanming ZhangKangkang GuoTaylor & Francis Grouparticleclassical swine fever virusmir-140rab253ʹ utrinhibitionInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 260-269 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	classical swine fever virus mir-140 rab25 3ʹ utr inhibition Infectious and parasitic diseases RC109-216
spellingShingle	classical swine fever virus mir-140 rab25 3ʹ utr inhibition Infectious and parasitic diseases RC109-216 Panpan Xu Shuangkai Jia Kai Wang Zhixin Fan Hongqing Zheng Jiangman Lv Yanfen Jiang Yufeng Hou Bihao Lou Hongchao Zhou Yanming Zhang Kangkang Guo MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells
description	Classical swine fever virus (CSFV) is one of the most important viral pathogens leading worldwide threats to pig industry. MicroRNAs (miRNAs) play important roles in regulating virus replication, but whether miRNAs affect CSFV infection is still poorly understood. In previous study, we identified four miRNAs that were down-regulated by CSFV in swine umbilical vein endothelial cells (SUVEC). In this study, miR-140, one of the most potently down-regulated genes was investigated. We found that the miRNA expression was significantly inhibited by CSFV infection. Subsequent studies revealed that miR-140 mimics signiﬁcantly inhibited CSFV replication, while the inhibition of endogenous miR-140 enhanced CSFV replication. By using bioinformatics prediction, luciferase reporter system, real-time fluorescence quantitative PCR (RT-qPCR) and Western blot assays, we further demonstrated that miR-140 bind to the 3ʹ UTR of Rab25 mRNA to regulate its expression. We also analyzed the expression pattern of Rab25 in SUVECs after CSFV infection. The results showed that CSFV infection induced Rab25 expression. Finally, Rab25 was found to promote CSFV replication. In conclusion, this study demonstrated that CSFV inhibits miR-140 expression and miR-140 inhibits replication by binding to host factor Rab25.
format	article
author	Panpan Xu Shuangkai Jia Kai Wang Zhixin Fan Hongqing Zheng Jiangman Lv Yanfen Jiang Yufeng Hou Bihao Lou Hongchao Zhou Yanming Zhang Kangkang Guo
author_facet	Panpan Xu Shuangkai Jia Kai Wang Zhixin Fan Hongqing Zheng Jiangman Lv Yanfen Jiang Yufeng Hou Bihao Lou Hongchao Zhou Yanming Zhang Kangkang Guo
author_sort	Panpan Xu
title	MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells
title_short	MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells
title_full	MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells
title_fullStr	MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells
title_full_unstemmed	MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells
title_sort	mir-140 inhibits classical swine fever virus replication by targeting rab25 in swine umbilical vein endothelial cells
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/ef501e4aca8841e1b79849b1c6b07bc3
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MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells

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