Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses

Abstract The tobacco virus resistance gene N contains four introns. Transient expression of transcripts from an N transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the...

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Autores principales:	Chihiro Ikeda, Kazuo Taku, Tsumugi Miyazaki, Rikako Shirai, Richard S. Nelson, Hiroshi Nyunoya, Yasuhiko Matsushita, Nobumitsu Sasaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef54fc9dce6c45e5b520ea82231e9ec7
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spelling	oai:doaj.org-article:ef54fc9dce6c45e5b520ea82231e9ec72021-12-02T18:47:06ZCooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses10.1038/s41598-021-94713-42045-2322https://doaj.org/article/ef54fc9dce6c45e5b520ea82231e9ec72021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94713-4https://doaj.org/toc/2045-2322Abstract The tobacco virus resistance gene N contains four introns. Transient expression of transcripts from an N transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the native promoter, the elicitor, or the individual introns for enhanced expression of N has not been fully studied. Here, we determined that 35S promoter-driven N transcript expression could be enhanced in the presence of the four introns regardless of the co-expression of the virus elicitor in tobacco. Function analyses using a series of N transgenes with different combination of introns revealed that the presence of intron 1 more so than intron 2 allowed higher accumulation of premature and mature N transcripts; however, both introns were important for not only enhanced gene expression but also for induction of cell death in tobacco and induced local resistance to spread of virus in Nicotiana benthamiana. Our findings indicate that introns 1 and 2 cooperatively contribute to N expression and virus resistance.Chihiro IkedaKazuo TakuTsumugi MiyazakiRikako ShiraiRichard S. NelsonHiroshi NyunoyaYasuhiko MatsushitaNobumitsu SasakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chihiro Ikeda Kazuo Taku Tsumugi Miyazaki Rikako Shirai Richard S. Nelson Hiroshi Nyunoya Yasuhiko Matsushita Nobumitsu Sasaki Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
description	Abstract The tobacco virus resistance gene N contains four introns. Transient expression of transcripts from an N transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the native promoter, the elicitor, or the individual introns for enhanced expression of N has not been fully studied. Here, we determined that 35S promoter-driven N transcript expression could be enhanced in the presence of the four introns regardless of the co-expression of the virus elicitor in tobacco. Function analyses using a series of N transgenes with different combination of introns revealed that the presence of intron 1 more so than intron 2 allowed higher accumulation of premature and mature N transcripts; however, both introns were important for not only enhanced gene expression but also for induction of cell death in tobacco and induced local resistance to spread of virus in Nicotiana benthamiana. Our findings indicate that introns 1 and 2 cooperatively contribute to N expression and virus resistance.
format	article
author	Chihiro Ikeda Kazuo Taku Tsumugi Miyazaki Rikako Shirai Richard S. Nelson Hiroshi Nyunoya Yasuhiko Matsushita Nobumitsu Sasaki
author_facet	Chihiro Ikeda Kazuo Taku Tsumugi Miyazaki Rikako Shirai Richard S. Nelson Hiroshi Nyunoya Yasuhiko Matsushita Nobumitsu Sasaki
author_sort	Chihiro Ikeda
title	Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
title_short	Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
title_full	Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
title_fullStr	Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
title_full_unstemmed	Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses
title_sort	cooperative roles of introns 1 and 2 of tobacco resistance gene n in enhanced n transcript expression and antiviral defense responses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ef54fc9dce6c45e5b520ea82231e9ec7
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Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses

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