RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes

Abstract Sarcoidosis is a complex systemic granulomatous disorder of unknown etiology. Genome-wide association studies have not been able to explain a causative role for nucleotide variation in its pathogenesis. The goal of the present study was to identify the gene expression profile and the cellul...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jaya Talreja, Pershang Farshi, Adnan Alazizi, Francesca Luca, Roger Pique-Regi, Lobelia Samavati
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef5c58facfd94e009f25b14524da8b3f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ef5c58facfd94e009f25b14524da8b3f
record_format	dspace
spelling	oai:doaj.org-article:ef5c58facfd94e009f25b14524da8b3f2021-12-02T16:06:34ZRNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes10.1038/s41598-017-02941-42045-2322https://doaj.org/article/ef5c58facfd94e009f25b14524da8b3f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02941-4https://doaj.org/toc/2045-2322Abstract Sarcoidosis is a complex systemic granulomatous disorder of unknown etiology. Genome-wide association studies have not been able to explain a causative role for nucleotide variation in its pathogenesis. The goal of the present study was to identify the gene expression profile and the cellular pathways altered in sarcoidosis monocytes via RNA-sequencing. Peripheral blood monocytes play a role in sarcoidosis inflammation. Therefore, we determined and compared the transcriptional signature of monocytes from peripheral blood from sarcoidosis patients and healthy controls via RNA-sequencing. We found 2,446 differentially expressed (DE) genes between sarcoidosis and healthy control monocytes. Analysis of these DE genes showed enrichment for ribosome, phagocytosis, lysosome, proteasome, oxidative phosphorylation and metabolic pathways. RNA-sequencing identified upregulation of genes involved in phagocytosis and lysosomal pathway in sarcoidosis monocytes, whereas genes involved in proteasome degradation and ribosomal pathways were downregulated. Further studies are needed to investigate the role of specific genes involved in the identified pathways and their possible interaction leading to sarcoidosis pathology.Jaya TalrejaPershang FarshiAdnan AlaziziFrancesca LucaRoger Pique-RegiLobelia SamavatiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jaya Talreja Pershang Farshi Adnan Alazizi Francesca Luca Roger Pique-Regi Lobelia Samavati RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
description	Abstract Sarcoidosis is a complex systemic granulomatous disorder of unknown etiology. Genome-wide association studies have not been able to explain a causative role for nucleotide variation in its pathogenesis. The goal of the present study was to identify the gene expression profile and the cellular pathways altered in sarcoidosis monocytes via RNA-sequencing. Peripheral blood monocytes play a role in sarcoidosis inflammation. Therefore, we determined and compared the transcriptional signature of monocytes from peripheral blood from sarcoidosis patients and healthy controls via RNA-sequencing. We found 2,446 differentially expressed (DE) genes between sarcoidosis and healthy control monocytes. Analysis of these DE genes showed enrichment for ribosome, phagocytosis, lysosome, proteasome, oxidative phosphorylation and metabolic pathways. RNA-sequencing identified upregulation of genes involved in phagocytosis and lysosomal pathway in sarcoidosis monocytes, whereas genes involved in proteasome degradation and ribosomal pathways were downregulated. Further studies are needed to investigate the role of specific genes involved in the identified pathways and their possible interaction leading to sarcoidosis pathology.
format	article
author	Jaya Talreja Pershang Farshi Adnan Alazizi Francesca Luca Roger Pique-Regi Lobelia Samavati
author_facet	Jaya Talreja Pershang Farshi Adnan Alazizi Francesca Luca Roger Pique-Regi Lobelia Samavati
author_sort	Jaya Talreja
title	RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
title_short	RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
title_full	RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
title_fullStr	RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
title_full_unstemmed	RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes
title_sort	rna-sequencing identifies novel pathways in sarcoidosis monocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef5c58facfd94e009f25b14524da8b3f
work_keys_str_mv	AT jayatalreja rnasequencingidentifiesnovelpathwaysinsarcoidosismonocytes AT pershangfarshi rnasequencingidentifiesnovelpathwaysinsarcoidosismonocytes AT adnanalazizi rnasequencingidentifiesnovelpathwaysinsarcoidosismonocytes AT francescaluca rnasequencingidentifiesnovelpathwaysinsarcoidosismonocytes AT rogerpiqueregi rnasequencingidentifiesnovelpathwaysinsarcoidosismonocytes AT lobeliasamavati rnasequencingidentifiesnovelpathwaysinsarcoidosismonocytes
_version_	1718384999953072128

RNA-sequencing Identifies Novel Pathways in Sarcoidosis Monocytes

Ejemplares similares