Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus

BACKGROUND AND OBJECTIVE: The rabies virus infection leads to 60,000 deaths worldwide each year and is considered an economic and social hazard. Although our knowledge of the rabies virus replication and its interaction with the host cell is in its infancy, it helps us design optimal drugs and vacci...

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Autores principales:	F Shirghasemi Alalan, R Hajihosseini, A Rasooli, M Fazeli
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2021
Materias:	rabies virus oxidative stress message transmission. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ef5c9252cf0644cba94c4329fa369f4d
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spelling	oai:doaj.org-article:ef5c9252cf0644cba94c4329fa369f4d2021-11-29T07:40:49ZEvaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus1561-41072251-7170https://doaj.org/article/ef5c9252cf0644cba94c4329fa369f4d2021-03-01T00:00:00Zhttp://jbums.org/article-1-9198-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: The rabies virus infection leads to 60,000 deaths worldwide each year and is considered an economic and social hazard. Although our knowledge of the rabies virus replication and its interaction with the host cell is in its infancy, it helps us design optimal drugs and vaccines. Therefore, the aim of this study was to evaluate the changes in catalase and superoxide dismutase (SOD) enzymes, nitric oxide (NO) chemical composition and NF-κB gene activity as biomarkers of oxidative stress response in brain tissue of mice infected with street rabies virus. METHODS: In this experimental study, 16 21-day-old outbred mice were divided into two groups of healthy and experimental (n=8). After determining the titer of rabies virus, the mice in the experimental group received 0.03 ml of rabies virus with LD50 intracerebrally (IC) using Hamilton 0.25 ml syringe. To evaluate the changes of catalase, NO, and SOD enzymes, test kit and colorimetric method were used and NF-kB activity was evaluated by Real-Time PCR according to Sdha gene. FINDINGS: In this study, catalase activity in the brains of infected mice (0.14±0.007) was significantly reduced compared to healthy mice (control) (0.67±0.008) (p=0.01). In this experimental study, the SOD activity in the brains of infected mice (3±0.2 nmol/min/ml) was significantly reduced compared to healthy mice (control) (5.7 nmol/min/ml) (p=0.035). Evaluation of NO accumulation in the brain tissue of the infected group (9.0±0.04 mU/mg) was significantly increased compared to the healthy group (9.04±0.03 mU/mg) (p=0.025). Furthermore, the expression level of NF-κB in the brain of the mice infected with rabies virus was one tenth of the expression of this gene in the brain of healthy mice. CONCLUSION: The results showed that rabies virus decreased the activity of catalase and SOD enzymes and increased nitric oxide. Furthermore, NF-κB expression decreased in the brain of mice infected with rabies virus, which may be due to the effect of the virus on the cellular signaling pathway.F Shirghasemi AlalanR HajihosseiniA RasooliM FazeliBabol University of Medical Sciencesarticlerabies virusoxidative stressmessage transmission.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 23, Iss 1, Pp 38-45 (2021)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	rabies virus oxidative stress message transmission. Medicine R Medicine (General) R5-920
spellingShingle	rabies virus oxidative stress message transmission. Medicine R Medicine (General) R5-920 F Shirghasemi Alalan R Hajihosseini A Rasooli M Fazeli Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus
description	BACKGROUND AND OBJECTIVE: The rabies virus infection leads to 60,000 deaths worldwide each year and is considered an economic and social hazard. Although our knowledge of the rabies virus replication and its interaction with the host cell is in its infancy, it helps us design optimal drugs and vaccines. Therefore, the aim of this study was to evaluate the changes in catalase and superoxide dismutase (SOD) enzymes, nitric oxide (NO) chemical composition and NF-κB gene activity as biomarkers of oxidative stress response in brain tissue of mice infected with street rabies virus. METHODS: In this experimental study, 16 21-day-old outbred mice were divided into two groups of healthy and experimental (n=8). After determining the titer of rabies virus, the mice in the experimental group received 0.03 ml of rabies virus with LD50 intracerebrally (IC) using Hamilton 0.25 ml syringe. To evaluate the changes of catalase, NO, and SOD enzymes, test kit and colorimetric method were used and NF-kB activity was evaluated by Real-Time PCR according to Sdha gene. FINDINGS: In this study, catalase activity in the brains of infected mice (0.14±0.007) was significantly reduced compared to healthy mice (control) (0.67±0.008) (p=0.01). In this experimental study, the SOD activity in the brains of infected mice (3±0.2 nmol/min/ml) was significantly reduced compared to healthy mice (control) (5.7 nmol/min/ml) (p=0.035). Evaluation of NO accumulation in the brain tissue of the infected group (9.0±0.04 mU/mg) was significantly increased compared to the healthy group (9.04±0.03 mU/mg) (p=0.025). Furthermore, the expression level of NF-κB in the brain of the mice infected with rabies virus was one tenth of the expression of this gene in the brain of healthy mice. CONCLUSION: The results showed that rabies virus decreased the activity of catalase and SOD enzymes and increased nitric oxide. Furthermore, NF-κB expression decreased in the brain of mice infected with rabies virus, which may be due to the effect of the virus on the cellular signaling pathway.
format	article
author	F Shirghasemi Alalan R Hajihosseini A Rasooli M Fazeli
author_facet	F Shirghasemi Alalan R Hajihosseini A Rasooli M Fazeli
author_sort	F Shirghasemi Alalan
title	Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus
title_short	Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus
title_full	Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus
title_fullStr	Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus
title_full_unstemmed	Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus
title_sort	evaluation of changes in biomarkers of oxidative stress response in the brain of mice infected with street rabies virus
publisher	Babol University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/ef5c9252cf0644cba94c4329fa369f4d
work_keys_str_mv	AT fshirghasemialalan evaluationofchangesinbiomarkersofoxidativestressresponseinthebrainofmiceinfectedwithstreetrabiesvirus AT rhajihosseini evaluationofchangesinbiomarkersofoxidativestressresponseinthebrainofmiceinfectedwithstreetrabiesvirus AT arasooli evaluationofchangesinbiomarkersofoxidativestressresponseinthebrainofmiceinfectedwithstreetrabiesvirus AT mfazeli evaluationofchangesinbiomarkersofoxidativestressresponseinthebrainofmiceinfectedwithstreetrabiesvirus
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Evaluation of Changes in Biomarkers of Oxidative Stress Response in the Brain of Mice Infected with Street Rabies Virus

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