First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa

Abstract Background Due to the lack of vaccines, malaria control mainly involves the control of anopheline vectors (Anopheles spp.) using chemical insecticides. However, the prolonged and indiscriminate use of these compounds has led to the emergence of resistance in Anopheles populations in Africa....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Derciliano Lopes da Cruz, Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Elainne Christine de Souza Gomes, Silvia Gonçalves Pires, Lara Ferrero Gomez, Constância Flávia Junqueira Ayres
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Malaria Anopheles arabiensis Knockdown resistance Kdr mutation Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/ef6e26ad59e547c28c214a2cde03ccf9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ef6e26ad59e547c28c214a2cde03ccf9
record_format	dspace
spelling	oai:doaj.org-article:ef6e26ad59e547c28c214a2cde03ccf92021-11-28T12:22:56ZFirst report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa10.1186/s13071-021-05088-41756-3305https://doaj.org/article/ef6e26ad59e547c28c214a2cde03ccf92021-11-01T00:00:00Zhttps://doi.org/10.1186/s13071-021-05088-4https://doaj.org/toc/1756-3305Abstract Background Due to the lack of vaccines, malaria control mainly involves the control of anopheline vectors (Anopheles spp.) using chemical insecticides. However, the prolonged and indiscriminate use of these compounds has led to the emergence of resistance in Anopheles populations in Africa. Insecticide resistance surveillance programs are less frequent in Cabo Verde than in other African countries. This study aimed to investigate the circulation of the L1014F and L1014S alleles in natural populations of Anopheles arabiensis collected from two sampling sites in the city of Praia, Cabo Verde. Methods Anopheles larvae were collected from the two sampling sites and reared in the laboratory until the adult stage. Mosquitoes were first morphologically identified by classical taxonomy and then by molecular species identification using molecular markers. All Anopheles arabiensis were subjected to PCR analysis to screen for mutations associated to resistance in the Na v gene. Results A total of 105 mosquitoes, all belonging to the Anopheles gambiae complex, were identified by classical taxonomy as well as by molecular taxonomy. Molecular identification showed that 100% of the An. gambiae senso lato specimens analyzed corresponded to An. arabiensis. Analysis of the Na v gene revealed the presence of L1014S and L1014F alleles with frequencies of 0.10 and 0.19, respectively. Conclusions Our data demonstrated, for the first time, the presence of the L1014F allele in the An. arabiensis population from Cabo Verde, as well as an increase in the frequency of the kdr L1014S allele reported in a previous study. The results of this study demonstrate the need to establish new approaches in vector control programs in Cabo Verde. Graphical AbstractDerciliano Lopes da CruzMarcelo Henrique Santos PaivaDuschinka Ribeiro Duarte GuedesElainne Christine de Souza GomesSilvia Gonçalves PiresLara Ferrero GomezConstância Flávia Junqueira AyresBMCarticleMalariaAnopheles arabiensisKnockdown resistanceKdr mutationInfectious and parasitic diseasesRC109-216ENParasites & Vectors, Vol 14, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Malaria Anopheles arabiensis Knockdown resistance Kdr mutation Infectious and parasitic diseases RC109-216
spellingShingle	Malaria Anopheles arabiensis Knockdown resistance Kdr mutation Infectious and parasitic diseases RC109-216 Derciliano Lopes da Cruz Marcelo Henrique Santos Paiva Duschinka Ribeiro Duarte Guedes Elainne Christine de Souza Gomes Silvia Gonçalves Pires Lara Ferrero Gomez Constância Flávia Junqueira Ayres First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
description	Abstract Background Due to the lack of vaccines, malaria control mainly involves the control of anopheline vectors (Anopheles spp.) using chemical insecticides. However, the prolonged and indiscriminate use of these compounds has led to the emergence of resistance in Anopheles populations in Africa. Insecticide resistance surveillance programs are less frequent in Cabo Verde than in other African countries. This study aimed to investigate the circulation of the L1014F and L1014S alleles in natural populations of Anopheles arabiensis collected from two sampling sites in the city of Praia, Cabo Verde. Methods Anopheles larvae were collected from the two sampling sites and reared in the laboratory until the adult stage. Mosquitoes were first morphologically identified by classical taxonomy and then by molecular species identification using molecular markers. All Anopheles arabiensis were subjected to PCR analysis to screen for mutations associated to resistance in the Na v gene. Results A total of 105 mosquitoes, all belonging to the Anopheles gambiae complex, were identified by classical taxonomy as well as by molecular taxonomy. Molecular identification showed that 100% of the An. gambiae senso lato specimens analyzed corresponded to An. arabiensis. Analysis of the Na v gene revealed the presence of L1014S and L1014F alleles with frequencies of 0.10 and 0.19, respectively. Conclusions Our data demonstrated, for the first time, the presence of the L1014F allele in the An. arabiensis population from Cabo Verde, as well as an increase in the frequency of the kdr L1014S allele reported in a previous study. The results of this study demonstrate the need to establish new approaches in vector control programs in Cabo Verde. Graphical Abstract
format	article
author	Derciliano Lopes da Cruz Marcelo Henrique Santos Paiva Duschinka Ribeiro Duarte Guedes Elainne Christine de Souza Gomes Silvia Gonçalves Pires Lara Ferrero Gomez Constância Flávia Junqueira Ayres
author_facet	Derciliano Lopes da Cruz Marcelo Henrique Santos Paiva Duschinka Ribeiro Duarte Guedes Elainne Christine de Souza Gomes Silvia Gonçalves Pires Lara Ferrero Gomez Constância Flávia Junqueira Ayres
author_sort	Derciliano Lopes da Cruz
title	First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
title_short	First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
title_full	First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
title_fullStr	First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
title_full_unstemmed	First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
title_sort	first report of the l1014f kdr mutation in wild populations of anopheles arabiensis in cabo verde, west africa
publisher	BMC
publishDate	2021
url	https://doaj.org/article/ef6e26ad59e547c28c214a2cde03ccf9
work_keys_str_mv	AT dercilianolopesdacruz firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica AT marcelohenriquesantospaiva firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica AT duschinkaribeiroduarteguedes firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica AT elainnechristinedesouzagomes firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica AT silviagoncalvespires firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica AT laraferrerogomez firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica AT constanciaflaviajunqueiraayres firstreportofthel1014fkdrmutationinwildpopulationsofanophelesarabiensisincaboverdewestafrica
_version_	1718408053676572672

First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa

Ejemplares similares