Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish

Abstract Estrogen plays fundamental roles in a range of developmental processes and exposure to estrogen mimicking chemicals has been associated with various adverse health effects in both wildlife and human populations. Estrogenic chemicals are found commonly as mixtures in the environment and can...

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Autores principales:	Jon M. Green, Anke Lange, Aaron Scott, Maciej Trznadel, Htoo Aung Wai, Aya Takesono, A. Ross Brown, Stewart F. Owen, Tetsuhiro Kudoh, Charles R. Tyler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef7241818e8642a0b0aef1a15524e029
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spelling	oai:doaj.org-article:ef7241818e8642a0b0aef1a15524e0292021-12-02T11:40:36ZEarly life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish10.1038/s41598-018-20922-z2045-2322https://doaj.org/article/ef7241818e8642a0b0aef1a15524e0292018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20922-zhttps://doaj.org/toc/2045-2322Abstract Estrogen plays fundamental roles in a range of developmental processes and exposure to estrogen mimicking chemicals has been associated with various adverse health effects in both wildlife and human populations. Estrogenic chemicals are found commonly as mixtures in the environment and can have additive effects, however risk analysis is typically conducted for single-chemicals with little, or no, consideration given for an animal’s exposure history. Here we developed a transgenic zebrafish with a photoconvertable fluorophore (Kaede, green to red on UV light exposure) in a skin pigment-free mutant element (ERE)-Kaede-Casper model and applied it to quantify tissue-specific fluorescence biosensor responses for combinations of estrogen exposures during early life using fluorescence microscopy and image analysis. We identify windows of tissue-specific sensitivity to ethinylestradiol (EE2) for exposure during early-life (0–5 dpf) and illustrate that exposure to estrogen (EE2) during 0–48 hpf enhances responsiveness (sensitivity) to different environmental estrogens (EE2, genistein and bisphenol A) for subsequent exposures during development. Our findings illustrate the importance of an organism’s stage of development and estrogen exposure history for assessments on, and possible health risks associated with, estrogen exposure.Jon M. GreenAnke LangeAaron ScottMaciej TrznadelHtoo Aung WaiAya TakesonoA. Ross BrownStewart F. OwenTetsuhiro KudohCharles R. TylerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jon M. Green Anke Lange Aaron Scott Maciej Trznadel Htoo Aung Wai Aya Takesono A. Ross Brown Stewart F. Owen Tetsuhiro Kudoh Charles R. Tyler Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
description	Abstract Estrogen plays fundamental roles in a range of developmental processes and exposure to estrogen mimicking chemicals has been associated with various adverse health effects in both wildlife and human populations. Estrogenic chemicals are found commonly as mixtures in the environment and can have additive effects, however risk analysis is typically conducted for single-chemicals with little, or no, consideration given for an animal’s exposure history. Here we developed a transgenic zebrafish with a photoconvertable fluorophore (Kaede, green to red on UV light exposure) in a skin pigment-free mutant element (ERE)-Kaede-Casper model and applied it to quantify tissue-specific fluorescence biosensor responses for combinations of estrogen exposures during early life using fluorescence microscopy and image analysis. We identify windows of tissue-specific sensitivity to ethinylestradiol (EE2) for exposure during early-life (0–5 dpf) and illustrate that exposure to estrogen (EE2) during 0–48 hpf enhances responsiveness (sensitivity) to different environmental estrogens (EE2, genistein and bisphenol A) for subsequent exposures during development. Our findings illustrate the importance of an organism’s stage of development and estrogen exposure history for assessments on, and possible health risks associated with, estrogen exposure.
format	article
author	Jon M. Green Anke Lange Aaron Scott Maciej Trznadel Htoo Aung Wai Aya Takesono A. Ross Brown Stewart F. Owen Tetsuhiro Kudoh Charles R. Tyler
author_facet	Jon M. Green Anke Lange Aaron Scott Maciej Trznadel Htoo Aung Wai Aya Takesono A. Ross Brown Stewart F. Owen Tetsuhiro Kudoh Charles R. Tyler
author_sort	Jon M. Green
title	Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
title_short	Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
title_full	Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
title_fullStr	Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
title_full_unstemmed	Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
title_sort	early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ef7241818e8642a0b0aef1a15524e029
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Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish

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