Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles

Abstract The evolution of bioluminescence has prompted scientific attention to illuminate phylogenetic relationships of luminescent beetles. However, genomic resources are virtually lacking in rhagophthalmids (Rhagophthalmidae) and their related firefly beetles lampyrids (Lampyridae). Here, we emplo...

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Autores principales:	Kai Wang, Wei Hong, Hengwu Jiao, Huabin Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef85b31b9e304297ac66df36293f0393
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spelling	oai:doaj.org-article:ef85b31b9e304297ac66df36293f03932021-12-02T11:52:30ZTranscriptome sequencing and phylogenetic analysis of four species of luminescent beetles10.1038/s41598-017-01835-92045-2322https://doaj.org/article/ef85b31b9e304297ac66df36293f03932017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01835-9https://doaj.org/toc/2045-2322Abstract The evolution of bioluminescence has prompted scientific attention to illuminate phylogenetic relationships of luminescent beetles. However, genomic resources are virtually lacking in rhagophthalmids (Rhagophthalmidae) and their related firefly beetles lampyrids (Lampyridae). Here, we employed the Illumina Hiseq 2000 platform and sequenced the whole-body transcriptomes of the four luminescent beetles: one rhagophthalmid (Rhagophthalmus sp.) and three fireflies (Asymmetricata circumdata, Aquatica ficta, and Pyrocoelia pectoralis). We obtained 55.4, 43.4, 38.6, and 36.7 million clean reads for the four species, respectively. All reads were assembled into contigs from which unigenes were derived. All unigenes were annotated by publicly available databases, and a total of 4325 orthologous genes were identified. Using multiple phylogenetic approaches, our transcriptome data confirmed the distinctiveness of Rhagophthalmidae from Lampyridae, which was also supported by our mitogenome analysis using three newly determined mitogenome sequences and 12 previously published ones. Together, this study is the first report of whole transcriptome sequencing data in Rhagophthalmidae and Lampyridae species, representing a valuable genomic resource for studying the origin and evolution of some remarkable traits in these beetles such as bioluminescence. Moreover, our transcriptome and mitogenome data provide useful phylogenetic information that could be of importance in future studies of phylogenetic inference.Kai WangWei HongHengwu JiaoHuabin ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kai Wang Wei Hong Hengwu Jiao Huabin Zhao Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
description	Abstract The evolution of bioluminescence has prompted scientific attention to illuminate phylogenetic relationships of luminescent beetles. However, genomic resources are virtually lacking in rhagophthalmids (Rhagophthalmidae) and their related firefly beetles lampyrids (Lampyridae). Here, we employed the Illumina Hiseq 2000 platform and sequenced the whole-body transcriptomes of the four luminescent beetles: one rhagophthalmid (Rhagophthalmus sp.) and three fireflies (Asymmetricata circumdata, Aquatica ficta, and Pyrocoelia pectoralis). We obtained 55.4, 43.4, 38.6, and 36.7 million clean reads for the four species, respectively. All reads were assembled into contigs from which unigenes were derived. All unigenes were annotated by publicly available databases, and a total of 4325 orthologous genes were identified. Using multiple phylogenetic approaches, our transcriptome data confirmed the distinctiveness of Rhagophthalmidae from Lampyridae, which was also supported by our mitogenome analysis using three newly determined mitogenome sequences and 12 previously published ones. Together, this study is the first report of whole transcriptome sequencing data in Rhagophthalmidae and Lampyridae species, representing a valuable genomic resource for studying the origin and evolution of some remarkable traits in these beetles such as bioluminescence. Moreover, our transcriptome and mitogenome data provide useful phylogenetic information that could be of importance in future studies of phylogenetic inference.
format	article
author	Kai Wang Wei Hong Hengwu Jiao Huabin Zhao
author_facet	Kai Wang Wei Hong Hengwu Jiao Huabin Zhao
author_sort	Kai Wang
title	Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
title_short	Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
title_full	Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
title_fullStr	Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
title_full_unstemmed	Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
title_sort	transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef85b31b9e304297ac66df36293f0393
work_keys_str_mv	AT kaiwang transcriptomesequencingandphylogeneticanalysisoffourspeciesofluminescentbeetles AT weihong transcriptomesequencingandphylogeneticanalysisoffourspeciesofluminescentbeetles AT hengwujiao transcriptomesequencingandphylogeneticanalysisoffourspeciesofluminescentbeetles AT huabinzhao transcriptomesequencingandphylogeneticanalysisoffourspeciesofluminescentbeetles
_version_	1718395080922890240

Transcriptome sequencing and phylogenetic analysis of four species of luminescent beetles

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