Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress

Background. Kidneys are extremely sensitive to various environmental factors. Stress disturbs prooxidant-antioxidant balance, causes hyperproduction of reactive oxygen species, changes activity of the nitroxidergic system components, regulating apoptosis. The use of mesenchymal stem cells can normal...

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Autores principales:	Elena V. Demianenko, Aleksandr I. Glukhov, Galina K. Gryzunova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2019
Materias:	stress apoptosis nitric oxide dna fragmentation mesenchymal stem cells Science Q
Acceso en línea:	https://doaj.org/article/ef8877faa94e4d9cba89a6bde918b684
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spelling	oai:doaj.org-article:ef8877faa94e4d9cba89a6bde918b6842021-11-23T06:14:42ZEffect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress2541-94202587-959610.29413/ABS.2019-4.1.21https://doaj.org/article/ef8877faa94e4d9cba89a6bde918b6842019-04-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1991https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Kidneys are extremely sensitive to various environmental factors. Stress disturbs prooxidant-antioxidant balance, causes hyperproduction of reactive oxygen species, changes activity of the nitroxidergic system components, regulating apoptosis. The use of mesenchymal stem cells can normalize functioning of damaged organs in the pathological process.Aim: to assess the effectiveness of mesenchymal stem cells in a single 24-hour immobilization according to the dynamics of apoptosis indices in renal tissue – nitric oxide (NO) and fragmented DNA.Materials and methods. The study included male nonlinear white rats aged 3 to 4 months and weighing 225 ± 25 grams. Experimental stress was modeled by the immobilization of animals in the fixation chambers within 24 hours. The efficacy of cell therapy was determined by the change in the concentration of the tested substances at 1, 3, 7, 14, 21 and 30 days of the experiment.Results. There was a sharp increase in the total amount of nitrates / nitrites and the level of DNA fragmentation in the homogenates of the renal parenchyma after the action of an acute stressor, which may indicate the induction of apoptosis. It was proved that in animals, receiving mesenchymal stem cells as a treatment, the restoration of the studied parameters in the kidney tissue was significantly accelerated in comparison with the controls values.Conclusion. Mesenchymal stem cells protect cells from self-destruction and activate reparation, which makes them promising for further study.Elena V. DemianenkoAleksandr I. GlukhovGalina K. GryzunovaScientific Сentre for Family Health and Human Reproduction Problemsarticlestressapoptosisnitric oxidedna fragmentationmesenchymal stem cellsScienceQRUActa Biomedica Scientifica, Vol 4, Iss 1, Pp 138-142 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	stress apoptosis nitric oxide dna fragmentation mesenchymal stem cells Science Q
spellingShingle	stress apoptosis nitric oxide dna fragmentation mesenchymal stem cells Science Q Elena V. Demianenko Aleksandr I. Glukhov Galina K. Gryzunova Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress
description	Background. Kidneys are extremely sensitive to various environmental factors. Stress disturbs prooxidant-antioxidant balance, causes hyperproduction of reactive oxygen species, changes activity of the nitroxidergic system components, regulating apoptosis. The use of mesenchymal stem cells can normalize functioning of damaged organs in the pathological process.Aim: to assess the effectiveness of mesenchymal stem cells in a single 24-hour immobilization according to the dynamics of apoptosis indices in renal tissue – nitric oxide (NO) and fragmented DNA.Materials and methods. The study included male nonlinear white rats aged 3 to 4 months and weighing 225 ± 25 grams. Experimental stress was modeled by the immobilization of animals in the fixation chambers within 24 hours. The efficacy of cell therapy was determined by the change in the concentration of the tested substances at 1, 3, 7, 14, 21 and 30 days of the experiment.Results. There was a sharp increase in the total amount of nitrates / nitrites and the level of DNA fragmentation in the homogenates of the renal parenchyma after the action of an acute stressor, which may indicate the induction of apoptosis. It was proved that in animals, receiving mesenchymal stem cells as a treatment, the restoration of the studied parameters in the kidney tissue was significantly accelerated in comparison with the controls values.Conclusion. Mesenchymal stem cells protect cells from self-destruction and activate reparation, which makes them promising for further study.
format	article
author	Elena V. Demianenko Aleksandr I. Glukhov Galina K. Gryzunova
author_facet	Elena V. Demianenko Aleksandr I. Glukhov Galina K. Gryzunova
author_sort	Elena V. Demianenko
title	Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress
title_short	Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress
title_full	Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress
title_fullStr	Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress
title_full_unstemmed	Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress
title_sort	effect of mesenchimal stem cells on apoptosis indices in renal parenchyma during experimental stress
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2019
url	https://doaj.org/article/ef8877faa94e4d9cba89a6bde918b684
work_keys_str_mv	AT elenavdemianenko effectofmesenchimalstemcellsonapoptosisindicesinrenalparenchymaduringexperimentalstress AT aleksandriglukhov effectofmesenchimalstemcellsonapoptosisindicesinrenalparenchymaduringexperimentalstress AT galinakgryzunova effectofmesenchimalstemcellsonapoptosisindicesinrenalparenchymaduringexperimentalstress
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Effect of Mesenchimal Stem Cells on Apoptosis Indices in Renal Parenchyma during Experimental Stress

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