Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.

White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplifica...

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Autores principales:	Xiaoming Xia, Yongxin Yu, Manfred Weidmann, Yingjie Pan, Shuling Yan, Yongjie Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef8896b4d6044c969afd0c264cf6e782
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spelling	oai:doaj.org-article:ef8896b4d6044c969afd0c264cf6e7822021-11-25T06:04:43ZRapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.1932-620310.1371/journal.pone.0104667https://doaj.org/article/ef8896b4d6044c969afd0c264cf6e7822014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25121957/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41 ± 0.17 min at 39 °C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.Xiaoming XiaYongxin YuManfred WeidmannYingjie PanShuling YanYongjie WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104667 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoming Xia Yongxin Yu Manfred Weidmann Yingjie Pan Shuling Yan Yongjie Wang Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
description	White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41 ± 0.17 min at 39 °C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.
format	article
author	Xiaoming Xia Yongxin Yu Manfred Weidmann Yingjie Pan Shuling Yan Yongjie Wang
author_facet	Xiaoming Xia Yongxin Yu Manfred Weidmann Yingjie Pan Shuling Yan Yongjie Wang
author_sort	Xiaoming Xia
title	Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
title_short	Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
title_full	Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
title_fullStr	Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
title_full_unstemmed	Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
title_sort	rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ef8896b4d6044c969afd0c264cf6e782
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Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay.

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