Klebsiella species associated with bovine mastitis in Newfoundland.

Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were...

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Autores principales:	Milka P Podder, Laura Rogers, Peter K Daley, Greg P Keefe, Hugh G Whitney, Kapil Tahlan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e
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spelling	oai:doaj.org-article:ef88c6d637484cb4b0423b9fa9223c3e2021-11-25T06:02:27ZKlebsiella species associated with bovine mastitis in Newfoundland.1932-620310.1371/journal.pone.0106518https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25180510/?tool=EBIhttps://doaj.org/toc/1932-6203Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were misidentified as K. pneumoniae in a small number of clinical mastitis (CM) cases from Newfoundland. Results suggest that the currently used standard biochemical/phenotypic tests lack the sensitivity required to accurately discriminate among the three mentioned Gram negative bacteria. In addition, a single strain of K. variicola was associated with CM from one farm in the study as demonstrated by Random Amplified Polymorphic DNA (RAPD) PCR. To the best of our knowledge, K. variicola, which is normally found in the environment, has not been isolated previously from milk obtained from cows with CM. Therefore, it is possible that K. variicola was not detected in milk samples in the past due to the inability of standard tests to discriminate it from other Klebsiella species.Milka P PodderLaura RogersPeter K DaleyGreg P KeefeHugh G WhitneyKapil TahlanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106518 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan Klebsiella species associated with bovine mastitis in Newfoundland.
description	Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were misidentified as K. pneumoniae in a small number of clinical mastitis (CM) cases from Newfoundland. Results suggest that the currently used standard biochemical/phenotypic tests lack the sensitivity required to accurately discriminate among the three mentioned Gram negative bacteria. In addition, a single strain of K. variicola was associated with CM from one farm in the study as demonstrated by Random Amplified Polymorphic DNA (RAPD) PCR. To the best of our knowledge, K. variicola, which is normally found in the environment, has not been isolated previously from milk obtained from cows with CM. Therefore, it is possible that K. variicola was not detected in milk samples in the past due to the inability of standard tests to discriminate it from other Klebsiella species.
format	article
author	Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan
author_facet	Milka P Podder Laura Rogers Peter K Daley Greg P Keefe Hugh G Whitney Kapil Tahlan
author_sort	Milka P Podder
title	Klebsiella species associated with bovine mastitis in Newfoundland.
title_short	Klebsiella species associated with bovine mastitis in Newfoundland.
title_full	Klebsiella species associated with bovine mastitis in Newfoundland.
title_fullStr	Klebsiella species associated with bovine mastitis in Newfoundland.
title_full_unstemmed	Klebsiella species associated with bovine mastitis in Newfoundland.
title_sort	klebsiella species associated with bovine mastitis in newfoundland.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ef88c6d637484cb4b0423b9fa9223c3e
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Klebsiella species associated with bovine mastitis in Newfoundland.

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