Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.

Monocytes/macrophages are key players in all phases of physiological and pathological inflammation. To understanding the regulation of macrophage functional differentiation during inflammation, we designed an in vitro model that recapitulates the different phases of the reaction (recruitment, initia...

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Autores principales:	Paola Italiani, Emilia M C Mazza, Davide Lucchesi, Ingrid Cifola, Claudia Gemelli, Alexis Grande, Cristina Battaglia, Silvio Bicciato, Diana Boraschi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef8c976d1ec14ab68b93585bb725bc99
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spelling	oai:doaj.org-article:ef8c976d1ec14ab68b93585bb725bc992021-11-18T08:34:13ZTranscriptomic profiling of the development of the inflammatory response in human monocytes in vitro.1932-620310.1371/journal.pone.0087680https://doaj.org/article/ef8c976d1ec14ab68b93585bb725bc992014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498352/?tool=EBIhttps://doaj.org/toc/1932-6203Monocytes/macrophages are key players in all phases of physiological and pathological inflammation. To understanding the regulation of macrophage functional differentiation during inflammation, we designed an in vitro model that recapitulates the different phases of the reaction (recruitment, initiation, development, and resolution), based on human primary blood monocytes exposed to sequential changes in microenvironmental conditions. All reaction phases were profiled by transcriptomic microarray analysis. Distinct clusters of genes were identified that are differentially regulated through the different phases of inflammation. The gene sets defined by GSEA analysis revealed that the inflammatory phase was enriched in inflammatory pathways, while the resolution phase comprised pathways related to metabolism and gene rearrangement. By comparing gene clusters differentially expressed in monocytes vs. M1 and vs. M2 macrophages extracted from an in-house created meta-database, it was shown that cells in the model resemble M1 during the inflammatory phase and M2 during resolution. The validation of inflammatory and transcriptional factors by qPCR and ELISA confirmed the transcriptomic profiles in the different phases of inflammation. The accurate description of the development of the human inflammatory reaction provided by this in vitro kinetic model can help in identifying regulatory mechanisms in physiological conditions and during pathological derangements.Paola ItalianiEmilia M C MazzaDavide LucchesiIngrid CifolaClaudia GemelliAlexis GrandeCristina BattagliaSilvio BicciatoDiana BoraschiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87680 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paola Italiani Emilia M C Mazza Davide Lucchesi Ingrid Cifola Claudia Gemelli Alexis Grande Cristina Battaglia Silvio Bicciato Diana Boraschi Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
description	Monocytes/macrophages are key players in all phases of physiological and pathological inflammation. To understanding the regulation of macrophage functional differentiation during inflammation, we designed an in vitro model that recapitulates the different phases of the reaction (recruitment, initiation, development, and resolution), based on human primary blood monocytes exposed to sequential changes in microenvironmental conditions. All reaction phases were profiled by transcriptomic microarray analysis. Distinct clusters of genes were identified that are differentially regulated through the different phases of inflammation. The gene sets defined by GSEA analysis revealed that the inflammatory phase was enriched in inflammatory pathways, while the resolution phase comprised pathways related to metabolism and gene rearrangement. By comparing gene clusters differentially expressed in monocytes vs. M1 and vs. M2 macrophages extracted from an in-house created meta-database, it was shown that cells in the model resemble M1 during the inflammatory phase and M2 during resolution. The validation of inflammatory and transcriptional factors by qPCR and ELISA confirmed the transcriptomic profiles in the different phases of inflammation. The accurate description of the development of the human inflammatory reaction provided by this in vitro kinetic model can help in identifying regulatory mechanisms in physiological conditions and during pathological derangements.
format	article
author	Paola Italiani Emilia M C Mazza Davide Lucchesi Ingrid Cifola Claudia Gemelli Alexis Grande Cristina Battaglia Silvio Bicciato Diana Boraschi
author_facet	Paola Italiani Emilia M C Mazza Davide Lucchesi Ingrid Cifola Claudia Gemelli Alexis Grande Cristina Battaglia Silvio Bicciato Diana Boraschi
author_sort	Paola Italiani
title	Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
title_short	Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
title_full	Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
title_fullStr	Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
title_full_unstemmed	Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
title_sort	transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ef8c976d1ec14ab68b93585bb725bc99
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Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.

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