Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation

Abstract Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new s...

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Autores principales:	M. I. Rizzo, L. Tomao, S. Tedesco, M. Cajozzo, M. Esposito, C. De Stefanis, A. M. Ferranti, D. Mezzogori, A. Palmieri, G. Pozzato, M. Algeri, F. Locatelli, L. Leone, M. Zama
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Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ef91816d171c445f8e8bb94ee894105c
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spelling	oai:doaj.org-article:ef91816d171c445f8e8bb94ee894105c2021-12-02T16:08:06ZEngineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation10.1038/s41598-021-93951-w2045-2322https://doaj.org/article/ef91816d171c445f8e8bb94ee894105c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93951-whttps://doaj.org/toc/2045-2322Abstract Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies to restore palatal bone interruption by using tissue or organ-decellularized bioscaffolds seeded with host cells. Aim of this study was to set up a new natural scaffold deriving from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure based on Quantum Molecular Resonance (QMR) and then subjected to in vitro recellularization with human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Our results demonstrated the efficiency of decellularization treatment gaining a natural, non-immunogenic scaffold with preserved collagen microenvironment that displays a favorable support to hMSC engraftment, spreading and differentiation. Ultrastructural analysis showed that the micro-perforation procedure preserved the collagen mesh, increasing the osteoinductive potential for mesenchymal precursor cells. In conclusion, we developed a novel tissue engineering protocol to obtain a non-immunogenic mucoperiosteal scaffold suitable for allogenic transplantation and CL/P repair. The innovative micro-perforation procedure improving hMSC osteogenic differentiation potentially impacts for enhanced palatal bone regeneration leading to future clinical applications in humans.M. I. RizzoL. TomaoS. TedescoM. CajozzoM. EspositoC. De StefanisA. M. FerrantiD. MezzogoriA. PalmieriG. PozzatoM. AlgeriF. LocatelliL. LeoneM. ZamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. I. Rizzo L. Tomao S. Tedesco M. Cajozzo M. Esposito C. De Stefanis A. M. Ferranti D. Mezzogori A. Palmieri G. Pozzato M. Algeri F. Locatelli L. Leone M. Zama Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
description	Abstract Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies to restore palatal bone interruption by using tissue or organ-decellularized bioscaffolds seeded with host cells. Aim of this study was to set up a new natural scaffold deriving from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure based on Quantum Molecular Resonance (QMR) and then subjected to in vitro recellularization with human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Our results demonstrated the efficiency of decellularization treatment gaining a natural, non-immunogenic scaffold with preserved collagen microenvironment that displays a favorable support to hMSC engraftment, spreading and differentiation. Ultrastructural analysis showed that the micro-perforation procedure preserved the collagen mesh, increasing the osteoinductive potential for mesenchymal precursor cells. In conclusion, we developed a novel tissue engineering protocol to obtain a non-immunogenic mucoperiosteal scaffold suitable for allogenic transplantation and CL/P repair. The innovative micro-perforation procedure improving hMSC osteogenic differentiation potentially impacts for enhanced palatal bone regeneration leading to future clinical applications in humans.
format	article
author	M. I. Rizzo L. Tomao S. Tedesco M. Cajozzo M. Esposito C. De Stefanis A. M. Ferranti D. Mezzogori A. Palmieri G. Pozzato M. Algeri F. Locatelli L. Leone M. Zama
author_facet	M. I. Rizzo L. Tomao S. Tedesco M. Cajozzo M. Esposito C. De Stefanis A. M. Ferranti D. Mezzogori A. Palmieri G. Pozzato M. Algeri F. Locatelli L. Leone M. Zama
author_sort	M. I. Rizzo
title	Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_short	Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_full	Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_fullStr	Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_full_unstemmed	Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
title_sort	engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ef91816d171c445f8e8bb94ee894105c
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Engineered mucoperiosteal scaffold for cleft palate regeneration towards the non-immunogenic transplantation

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