Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients

The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) and immunocompromised patients is crucial for long-term protection. Immune response to SARS-CoV-2 infection was analysed in 57 IC and 15 solid organ transplanted (TX) patients. Antibody responses were dete...

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Autores principales: Federica Zavaglio, Vanessa Frangipane, Monica Morosini, Elisa Gabanti, Paola Zelini, Josè Camilla Sammartino, Alessandro Ferrari, Marilena Gregorini, Teresa Rampino, Annalia Asti, Elena Seminari, Angela Di Matteo, Barbara Cattadori, Carlo Pellegrini, Stelvio Tonello, Venkata Ramana Mallela, Rosalba Minisini, Manuela Rizzi, Pier Paolo Sainaghi, Federica Meloni, Daniele Lilleri, Fausto Baldanti
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Lenguaje:EN
Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:ef981fba17be46c6a56668952ed0ec6e2021-11-25T19:13:53ZRobust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients10.3390/v131122611999-4915https://doaj.org/article/ef981fba17be46c6a56668952ed0ec6e2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2261https://doaj.org/toc/1999-4915The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) and immunocompromised patients is crucial for long-term protection. Immune response to SARS-CoV-2 infection was analysed in 57 IC and 15 solid organ transplanted (TX) patients. Antibody responses were determined by ELISA and neutralization assay. T-cell response was determined by stimulation with peptide pools of the Spike, Envelope, Membrane, and Nucleocapsid proteins with a 20-h Activation Induced Marker (AIM) and 7-day lymphoproliferative assays. Antibody response was detected at similar levels in IC and TX patients. Anti-Spike IgG, IgA and neutralizing antibodies persisted for at least one year, while anti-Nucleocapsid IgG declined earlier. Patients with pneumonia developed higher antibody levels than patients with mild symptoms. Similarly, both rapid and proliferative T-cell responses were detected within the first two months after infection at comparable levels in IC and TX patients, and were higher in patients with pneumonia. T-cell response persisted for at least one year in both IC and TX patients. Spike, Membrane, and Nucleocapsid proteins elicited the major CD4<sup>+</sup> and CD8<sup>+</sup> T-cell responses, whereas the T-cell response to Envelope protein was negligible. After SARS-CoV-2 infection, antibody and T-cell responses develop rapidly and persist over time in both immunocompetent and transplanted patients.Federica ZavaglioVanessa FrangipaneMonica MorosiniElisa GabantiPaola ZeliniJosè Camilla SammartinoAlessandro FerrariMarilena GregoriniTeresa RampinoAnnalia AstiElena SeminariAngela Di MatteoBarbara CattadoriCarlo PellegriniStelvio TonelloVenkata Ramana MallelaRosalba MinisiniManuela RizziPier Paolo SainaghiFederica MeloniDaniele LilleriFausto BaldantiMDPI AGarticleSARS-CoV-2COVID-19immunocompetent patientstransplanted patientsspike proteinmembrane proteinMicrobiologyQR1-502ENViruses, Vol 13, Iss 2261, p 2261 (2021)
institution DOAJ
collection DOAJ
language EN
topic SARS-CoV-2
COVID-19
immunocompetent patients
transplanted patients
spike protein
membrane protein
Microbiology
QR1-502
spellingShingle SARS-CoV-2
COVID-19
immunocompetent patients
transplanted patients
spike protein
membrane protein
Microbiology
QR1-502
Federica Zavaglio
Vanessa Frangipane
Monica Morosini
Elisa Gabanti
Paola Zelini
Josè Camilla Sammartino
Alessandro Ferrari
Marilena Gregorini
Teresa Rampino
Annalia Asti
Elena Seminari
Angela Di Matteo
Barbara Cattadori
Carlo Pellegrini
Stelvio Tonello
Venkata Ramana Mallela
Rosalba Minisini
Manuela Rizzi
Pier Paolo Sainaghi
Federica Meloni
Daniele Lilleri
Fausto Baldanti
Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients
description The development and persistence of SARS-CoV-2-specific immune response in immunocompetent (IC) and immunocompromised patients is crucial for long-term protection. Immune response to SARS-CoV-2 infection was analysed in 57 IC and 15 solid organ transplanted (TX) patients. Antibody responses were determined by ELISA and neutralization assay. T-cell response was determined by stimulation with peptide pools of the Spike, Envelope, Membrane, and Nucleocapsid proteins with a 20-h Activation Induced Marker (AIM) and 7-day lymphoproliferative assays. Antibody response was detected at similar levels in IC and TX patients. Anti-Spike IgG, IgA and neutralizing antibodies persisted for at least one year, while anti-Nucleocapsid IgG declined earlier. Patients with pneumonia developed higher antibody levels than patients with mild symptoms. Similarly, both rapid and proliferative T-cell responses were detected within the first two months after infection at comparable levels in IC and TX patients, and were higher in patients with pneumonia. T-cell response persisted for at least one year in both IC and TX patients. Spike, Membrane, and Nucleocapsid proteins elicited the major CD4<sup>+</sup> and CD8<sup>+</sup> T-cell responses, whereas the T-cell response to Envelope protein was negligible. After SARS-CoV-2 infection, antibody and T-cell responses develop rapidly and persist over time in both immunocompetent and transplanted patients.
format article
author Federica Zavaglio
Vanessa Frangipane
Monica Morosini
Elisa Gabanti
Paola Zelini
Josè Camilla Sammartino
Alessandro Ferrari
Marilena Gregorini
Teresa Rampino
Annalia Asti
Elena Seminari
Angela Di Matteo
Barbara Cattadori
Carlo Pellegrini
Stelvio Tonello
Venkata Ramana Mallela
Rosalba Minisini
Manuela Rizzi
Pier Paolo Sainaghi
Federica Meloni
Daniele Lilleri
Fausto Baldanti
author_facet Federica Zavaglio
Vanessa Frangipane
Monica Morosini
Elisa Gabanti
Paola Zelini
Josè Camilla Sammartino
Alessandro Ferrari
Marilena Gregorini
Teresa Rampino
Annalia Asti
Elena Seminari
Angela Di Matteo
Barbara Cattadori
Carlo Pellegrini
Stelvio Tonello
Venkata Ramana Mallela
Rosalba Minisini
Manuela Rizzi
Pier Paolo Sainaghi
Federica Meloni
Daniele Lilleri
Fausto Baldanti
author_sort Federica Zavaglio
title Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients
title_short Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients
title_full Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients
title_fullStr Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients
title_full_unstemmed Robust and Persistent B- and T-Cell Responses after COVID-19 in Immunocompetent and Solid Organ Transplant Recipient Patients
title_sort robust and persistent b- and t-cell responses after covid-19 in immunocompetent and solid organ transplant recipient patients
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/ef981fba17be46c6a56668952ed0ec6e
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