The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice

Abstract Seed setting is an important trait that contributes to seed yield and relies greatly on starch accumulation. In this study, a sulfoquinovosyl transferase-like protein, designated as SQD2.2 involved in seed setting and flavonoid accumulation, was identified and characterized in rice. Rice SQ...

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Autores principales:	Xinqiao Zhan, Qingwen Shen, Xuemin Wang, Yueyun Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/efa2128334ac4be8bcdd103582680908
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spelling	oai:doaj.org-article:efa2128334ac4be8bcdd1035826809082021-12-02T11:52:33ZThe Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice10.1038/s41598-017-04002-22045-2322https://doaj.org/article/efa2128334ac4be8bcdd1035826809082017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04002-2https://doaj.org/toc/2045-2322Abstract Seed setting is an important trait that contributes to seed yield and relies greatly on starch accumulation. In this study, a sulfoquinovosyl transferase-like protein, designated as SQD2.2 involved in seed setting and flavonoid accumulation, was identified and characterized in rice. Rice SQD2.2 is localized to the cytoplasm, and the SQD2.2 transcript was highest in leaves. Rice SQD2.2-overexpressing (OE) plants exhibited a decreased seed setting rate and diminished tiller number simultaneously with an increased glycosidic flavonoid level compared with wild-type (WT) plants. SQD2.2 catalyzes the glycosylation of apigenin to produce apigenin 7-O-glucoside using uridine diphosphate-glucose (UDPG) as a sugar donor, but it failed to compensate for sulfoquinovosyldiacylglycerol (SQDG) synthesis in the Arabidopsis sqd2 mutant. Furthermore, apigenin 7-O-glucoside inhibited starch synthase (SS) activity in a concentration-dependent manner, and SQD2.2-OE plants exhibited reduced SS activity accompanied by a significant reduction in starch levels and an elevation in soluble sugar levels relative to WT plants. Both adenosine diphosphate-glucose (ADPG) and UDPG levels in SQD2.2-OE plants were notably lower than those in WT plants. Taken together, rice SQD2.2 exhibits a novel role in flavonoid synthesis and plays an important role in mediating sugar allocation between primary and secondary metabolism in rice.Xinqiao ZhanQingwen ShenXuemin WangYueyun HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xinqiao Zhan Qingwen Shen Xuemin Wang Yueyun Hong The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice
description	Abstract Seed setting is an important trait that contributes to seed yield and relies greatly on starch accumulation. In this study, a sulfoquinovosyl transferase-like protein, designated as SQD2.2 involved in seed setting and flavonoid accumulation, was identified and characterized in rice. Rice SQD2.2 is localized to the cytoplasm, and the SQD2.2 transcript was highest in leaves. Rice SQD2.2-overexpressing (OE) plants exhibited a decreased seed setting rate and diminished tiller number simultaneously with an increased glycosidic flavonoid level compared with wild-type (WT) plants. SQD2.2 catalyzes the glycosylation of apigenin to produce apigenin 7-O-glucoside using uridine diphosphate-glucose (UDPG) as a sugar donor, but it failed to compensate for sulfoquinovosyldiacylglycerol (SQDG) synthesis in the Arabidopsis sqd2 mutant. Furthermore, apigenin 7-O-glucoside inhibited starch synthase (SS) activity in a concentration-dependent manner, and SQD2.2-OE plants exhibited reduced SS activity accompanied by a significant reduction in starch levels and an elevation in soluble sugar levels relative to WT plants. Both adenosine diphosphate-glucose (ADPG) and UDPG levels in SQD2.2-OE plants were notably lower than those in WT plants. Taken together, rice SQD2.2 exhibits a novel role in flavonoid synthesis and plays an important role in mediating sugar allocation between primary and secondary metabolism in rice.
format	article
author	Xinqiao Zhan Qingwen Shen Xuemin Wang Yueyun Hong
author_facet	Xinqiao Zhan Qingwen Shen Xuemin Wang Yueyun Hong
author_sort	Xinqiao Zhan
title	The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice
title_short	The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice
title_full	The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice
title_fullStr	The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice
title_full_unstemmed	The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice
title_sort	sulfoquinovosyltransferase-like enzyme sqd2.2 is involved in flavonoid glycosylation, regulating sugar metabolism and seed setting in rice
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/efa2128334ac4be8bcdd103582680908
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The Sulfoquinovosyltransferase-like Enzyme SQD2.2 is Involved in Flavonoid Glycosylation, Regulating Sugar Metabolism and Seed Setting in Rice

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