An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection

Abstract There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the...

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Autores principales:	Hao Deng, Asanka Jayawardena, Jianxiong Chan, Sher Maine Tan, Tuncay Alan, Patrick Kwan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/efa47e451679434ea20503c01f13db5a
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spelling	oai:doaj.org-article:efa47e451679434ea20503c01f13db5a2021-12-02T16:31:52ZAn ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection10.1038/s41598-021-94652-02045-2322https://doaj.org/article/efa47e451679434ea20503c01f13db5a2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94652-0https://doaj.org/toc/2045-2322Abstract There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm3) unit. The test is easy to operate and does not require skilled personnel. The total time from sample to answer is approximately 35 min; a colorimetric readout indicates positive or negative results. This portable diagnostic platform has significant potential for rapid and effective testing in community settings. This will accelerate clinical decision making, in terms of effective triage and timely therapeutic and infection control interventions.Hao DengAsanka JayawardenaJianxiong ChanSher Maine TanTuncay AlanPatrick KwanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao Deng Asanka Jayawardena Jianxiong Chan Sher Maine Tan Tuncay Alan Patrick Kwan An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
description	Abstract There is currently a high level of demand for rapid COVID-19 tests, that can detect the onset of the disease at point of care settings. We have developed an ultra-portable, self-contained, point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection, based on the principle of loop mediated isothermal amplification (LAMP). The LAMP assay is 100% sensitive and specific to detect a minimum of 300 RNA copies/reaction of SARS-CoV-2. All of the required sample transportation, lysing and amplification steps are performed in a standalone disposable cartridge, which is controlled by a battery operated, pocket size (6x9x4cm3) unit. The test is easy to operate and does not require skilled personnel. The total time from sample to answer is approximately 35 min; a colorimetric readout indicates positive or negative results. This portable diagnostic platform has significant potential for rapid and effective testing in community settings. This will accelerate clinical decision making, in terms of effective triage and timely therapeutic and infection control interventions.
format	article
author	Hao Deng Asanka Jayawardena Jianxiong Chan Sher Maine Tan Tuncay Alan Patrick Kwan
author_facet	Hao Deng Asanka Jayawardena Jianxiong Chan Sher Maine Tan Tuncay Alan Patrick Kwan
author_sort	Hao Deng
title	An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
title_short	An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
title_full	An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
title_fullStr	An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
title_full_unstemmed	An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection
title_sort	ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active covid-19 infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/efa47e451679434ea20503c01f13db5a
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An ultra-portable, self-contained point-of-care nucleic acid amplification test for diagnosis of active COVID-19 infection

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