Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission.
<h4>Objective</h4>In current clinical practice, optimal treatment of inflammatory bowel disease (IBD) aims at the induction and maintenance of clinical remission. Clinical remission is apparent when laboratory markers of inflammation are normal and clinical symptoms are absent. However,...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/efa5ee04a5654b7d8d2d142109cfe77b |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:efa5ee04a5654b7d8d2d142109cfe77b |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:efa5ee04a5654b7d8d2d142109cfe77b2021-11-18T08:45:55ZGene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission.1932-620310.1371/journal.pone.0079549https://doaj.org/article/efa5ee04a5654b7d8d2d142109cfe77b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24260248/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>In current clinical practice, optimal treatment of inflammatory bowel disease (IBD) aims at the induction and maintenance of clinical remission. Clinical remission is apparent when laboratory markers of inflammation are normal and clinical symptoms are absent. However, sub-clinical inflammation can still be present. A detailed analysis of the immune status during this inactive state of disease may provide a useful tool to categorize patients with clinical remission into subsets with variable states of immune activation.<h4>Design</h4>By using Affymetrix GeneChips, we analysed RNA gene expression profiles of peripheral blood leukocytes from pediatric IBD patients in clinical remission and controls. We performed (un)supervised clustering analysis of IBD-associated genes and applied Ingenuity® pathway software to identify specific molecular profiles between patients.<h4>Results</h4>Pediatric IBD patients with disease in clinical remission display heterogeneously distributed gene expression profiles that are significantly distinct from controls. We identified three clusters of IBD patients, each displaying specific expression profiles of IBD-associated genes.<h4>Conclusion</h4>The expression of immune- and IBD-associated genes in peripheral blood leukocytes from pediatric IBD patients in clinical remission was different from healthy controls, indicating that sub-clinical immune mechanisms are still active during remission. As such, RNA profiling of peripheral blood may allow for non-invasive patient subclassification and new perspectives in treatment regimes of IBD patients in the future.Pieter P E van LieropSigrid M SwagemakersCharlotte I de BieSabine MiddendorpPeter van BaarlenJanneke N SamsomWilfred F J van IjckenJohanna C EscherPeter J van der SpekEdward E S NieuwenhuisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79549 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Pieter P E van Lierop Sigrid M Swagemakers Charlotte I de Bie Sabine Middendorp Peter van Baarlen Janneke N Samsom Wilfred F J van Ijcken Johanna C Escher Peter J van der Spek Edward E S Nieuwenhuis Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| description |
<h4>Objective</h4>In current clinical practice, optimal treatment of inflammatory bowel disease (IBD) aims at the induction and maintenance of clinical remission. Clinical remission is apparent when laboratory markers of inflammation are normal and clinical symptoms are absent. However, sub-clinical inflammation can still be present. A detailed analysis of the immune status during this inactive state of disease may provide a useful tool to categorize patients with clinical remission into subsets with variable states of immune activation.<h4>Design</h4>By using Affymetrix GeneChips, we analysed RNA gene expression profiles of peripheral blood leukocytes from pediatric IBD patients in clinical remission and controls. We performed (un)supervised clustering analysis of IBD-associated genes and applied Ingenuity® pathway software to identify specific molecular profiles between patients.<h4>Results</h4>Pediatric IBD patients with disease in clinical remission display heterogeneously distributed gene expression profiles that are significantly distinct from controls. We identified three clusters of IBD patients, each displaying specific expression profiles of IBD-associated genes.<h4>Conclusion</h4>The expression of immune- and IBD-associated genes in peripheral blood leukocytes from pediatric IBD patients in clinical remission was different from healthy controls, indicating that sub-clinical immune mechanisms are still active during remission. As such, RNA profiling of peripheral blood may allow for non-invasive patient subclassification and new perspectives in treatment regimes of IBD patients in the future. |
| format |
article |
| author |
Pieter P E van Lierop Sigrid M Swagemakers Charlotte I de Bie Sabine Middendorp Peter van Baarlen Janneke N Samsom Wilfred F J van Ijcken Johanna C Escher Peter J van der Spek Edward E S Nieuwenhuis |
| author_facet |
Pieter P E van Lierop Sigrid M Swagemakers Charlotte I de Bie Sabine Middendorp Peter van Baarlen Janneke N Samsom Wilfred F J van Ijcken Johanna C Escher Peter J van der Spek Edward E S Nieuwenhuis |
| author_sort |
Pieter P E van Lierop |
| title |
Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| title_short |
Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| title_full |
Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| title_fullStr |
Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| title_full_unstemmed |
Gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| title_sort |
gene expression analysis of peripheral cells for subclassification of pediatric inflammatory bowel disease in remission. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/efa5ee04a5654b7d8d2d142109cfe77b |
| work_keys_str_mv |
AT pieterpevanlierop geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT sigridmswagemakers geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT charlotteidebie geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT sabinemiddendorp geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT petervanbaarlen geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT jannekensamsom geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT wilfredfjvanijcken geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT johannacescher geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT peterjvanderspek geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission AT edwardesnieuwenhuis geneexpressionanalysisofperipheralcellsforsubclassificationofpediatricinflammatoryboweldiseaseinremission |
| _version_ |
1718421358930558976 |