Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)

Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni)...

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Autores principales:	Drew J. Duckett, Jack Sullivan, Stacy Pirro, Bryan C. Carstens
Formato:	article
Lenguaje:	EN
Publicado:	GigaScience Press 2021
Materias:	Electronic computers. Computer science QA75.5-76.95
Acceso en línea:	https://doaj.org/article/efae1650a94240f7b4125f45eaabf586
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spelling	oai:doaj.org-article:efae1650a94240f7b4125f45eaabf5862021-12-02T18:00:18ZGenomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)2709-47152709-471510.46471/gigabyte.19https://doaj.org/article/efae1650a94240f7b4125f45eaabf5862021-05-01T00:00:00Zhttps://gigabytejournal.com/articles/19https://doaj.org/toc/2709-4715https://doaj.org/toc/2709-4715Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni) and from the montane vole (Microtus montanus). The water vole genomes were sequenced with Illumina and 10× Chromium plus Illumina sequencing, resulting in assemblies with ∼1600,000 and ∼30,000 scaffolds, respectively. The montane vole was also assembled into ∼13,000 scaffolds using Illumina sequencing. Mitochondrial genome assemblies were also performed for both species. Structural and functional annotation for the best water vole nuclear genome resulted in ∼24,500 annotated genes, with 83% of these having functional annotations. Assembly quality statistics for our nuclear assemblies fall within the range of genomes previously published in the genus Microtus, making the water vole and montane vole genomes useful additions to currently available genomic resources.Drew J. DuckettJack SullivanStacy PirroBryan C. CarstensGigaScience PressarticleElectronic computers. Computer scienceQA75.5-76.95ENGigaByte, Vol 2021, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electronic computers. Computer science QA75.5-76.95
spellingShingle	Electronic computers. Computer science QA75.5-76.95 Drew J. Duckett Jack Sullivan Stacy Pirro Bryan C. Carstens Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
description	Voles of the genus Microtus are important research organisms, yet genomic resources are lacking. Such resources would benefit future studies of immunology, phylogeography, cryptic diversity, and more. We sequenced and assembled nuclear genomes from two subspecies of water vole (Microtus richardsoni) and from the montane vole (Microtus montanus). The water vole genomes were sequenced with Illumina and 10× Chromium plus Illumina sequencing, resulting in assemblies with ∼1600,000 and ∼30,000 scaffolds, respectively. The montane vole was also assembled into ∼13,000 scaffolds using Illumina sequencing. Mitochondrial genome assemblies were also performed for both species. Structural and functional annotation for the best water vole nuclear genome resulted in ∼24,500 annotated genes, with 83% of these having functional annotations. Assembly quality statistics for our nuclear assemblies fall within the range of genomes previously published in the genus Microtus, making the water vole and montane vole genomes useful additions to currently available genomic resources.
format	article
author	Drew J. Duckett Jack Sullivan Stacy Pirro Bryan C. Carstens
author_facet	Drew J. Duckett Jack Sullivan Stacy Pirro Bryan C. Carstens
author_sort	Drew J. Duckett
title	Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_short	Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_full	Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_fullStr	Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_full_unstemmed	Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)
title_sort	genomic resources for the north american water vole (microtus richardsoni) and the montane vole (microtus montanus)
publisher	GigaScience Press
publishDate	2021
url	https://doaj.org/article/efae1650a94240f7b4125f45eaabf586
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Genomic Resources for the North American Water Vole (Microtus richardsoni) and the Montane Vole (Microtus montanus)

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