PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.

Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer con...

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Autores principales:	Arunkumar Arumugam, Cole Markham, Saurabh S Aykar, Barbara Van Der Pol, Paula Dixon, Michelle Wu, Season Wong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/efaf416c58514a478dd4f22d2b056229
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spelling	oai:doaj.org-article:efaf416c58514a478dd4f22d2b0562292021-12-02T20:05:24ZPrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.1932-620310.1371/journal.pone.0251812https://doaj.org/article/efaf416c58514a478dd4f22d2b0562292021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251812https://doaj.org/toc/1932-6203Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO2 sensor, solenoid valve, heater, and thermistors. CO2 is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO2 supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consistent CO2 and temperature levels for over three days without manual interruption. The results from our culture plates and real-time PCR indicate that our incubator performed equally well when compared to a much more expensive commercial CO2 incubator. We have also demonstrated that the antibiotic susceptibility assay can be performed in this low-cost CO2 incubator. Our work also indicates that the system can be connected to incubator chambers of various chamber volumes.Arunkumar ArumugamCole MarkhamSaurabh S AykarBarbara Van Der PolPaula DixonMichelle WuSeason WongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251812 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arunkumar Arumugam Cole Markham Saurabh S Aykar Barbara Van Der Pol Paula Dixon Michelle Wu Season Wong PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.
description	Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO2 sensor, solenoid valve, heater, and thermistors. CO2 is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO2 supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consistent CO2 and temperature levels for over three days without manual interruption. The results from our culture plates and real-time PCR indicate that our incubator performed equally well when compared to a much more expensive commercial CO2 incubator. We have also demonstrated that the antibiotic susceptibility assay can be performed in this low-cost CO2 incubator. Our work also indicates that the system can be connected to incubator chambers of various chamber volumes.
format	article
author	Arunkumar Arumugam Cole Markham Saurabh S Aykar Barbara Van Der Pol Paula Dixon Michelle Wu Season Wong
author_facet	Arunkumar Arumugam Cole Markham Saurabh S Aykar Barbara Van Der Pol Paula Dixon Michelle Wu Season Wong
author_sort	Arunkumar Arumugam
title	PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.
title_short	PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.
title_full	PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.
title_fullStr	PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.
title_full_unstemmed	PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.
title_sort	printrlab incubator: a portable and low-cost co2 incubator based on an open-source 3d printer architecture.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/efaf416c58514a478dd4f22d2b056229
work_keys_str_mv	AT arunkumararumugam printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture AT colemarkham printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture AT saurabhsaykar printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture AT barbaravanderpol printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture AT pauladixon printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture AT michellewu printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture AT seasonwong printrlabincubatoraportableandlowcostco2incubatorbasedonanopensource3dprinterarchitecture
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PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture.

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