Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis

Abstract Secretomes produced by mesenchymal stromal cells (MSCs) were considered to be therapeutic potential. However, harvesting enough primary MSCs from tissue was time-consuming and costly, which impeded the application of MSCs secretomes. This study was to immortalize MSCs and compare the secret...

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Autores principales:	Lei Dou, Yan Wu, Qifang Yan, Jinhua Wang, Yan Zhang, Ping Ji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/efb4b168972b430793766baae7ec6ebe
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spelling	oai:doaj.org-article:efb4b168972b430793766baae7ec6ebe2021-12-02T11:40:23ZSecretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis10.1038/s41598-017-07467-32045-2322https://doaj.org/article/efb4b168972b430793766baae7ec6ebe2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07467-3https://doaj.org/toc/2045-2322Abstract Secretomes produced by mesenchymal stromal cells (MSCs) were considered to be therapeutic potential. However, harvesting enough primary MSCs from tissue was time-consuming and costly, which impeded the application of MSCs secretomes. This study was to immortalize MSCs and compare the secretomes profile of immortalized and original MSCs. Human dental follicle cells (DFCs) were isolated and immortalized using pMPH86. The secretome profile of immortalized DFCs (iDFCs) was investigated and compared using iTRAQ labeling combined with mass spectrometry (MS) quantitative proteomics. The MS data was analyzed using ProteinPilotTM software, and then bioinformatic analysis of identified proteins was done. A total of 2092 secreted proteins were detected in conditioned media of iDFCs. Compared with primary DFCs, 253 differently expressed proteins were found in iDFCs secretome (142 up-regulated and 111 down-regulated). Intensive bioinformatic analysis revealed that the majority of secreted proteins were involved in cellular process, metabolic process, biological regulation, cellular component organization or biogenesis, immune system process, developmental process, response to stimulus and signaling. Proteomic profile of cell secretome wasn’t largely affected after immortalization converted by this piggyBac immortalization system. The secretome of iDFCs may be a good candidate of primary DFCs for regenerative medicine.Lei DouYan WuQifang YanJinhua WangYan ZhangPing JiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lei Dou Yan Wu Qifang Yan Jinhua Wang Yan Zhang Ping Ji Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis
description	Abstract Secretomes produced by mesenchymal stromal cells (MSCs) were considered to be therapeutic potential. However, harvesting enough primary MSCs from tissue was time-consuming and costly, which impeded the application of MSCs secretomes. This study was to immortalize MSCs and compare the secretomes profile of immortalized and original MSCs. Human dental follicle cells (DFCs) were isolated and immortalized using pMPH86. The secretome profile of immortalized DFCs (iDFCs) was investigated and compared using iTRAQ labeling combined with mass spectrometry (MS) quantitative proteomics. The MS data was analyzed using ProteinPilotTM software, and then bioinformatic analysis of identified proteins was done. A total of 2092 secreted proteins were detected in conditioned media of iDFCs. Compared with primary DFCs, 253 differently expressed proteins were found in iDFCs secretome (142 up-regulated and 111 down-regulated). Intensive bioinformatic analysis revealed that the majority of secreted proteins were involved in cellular process, metabolic process, biological regulation, cellular component organization or biogenesis, immune system process, developmental process, response to stimulus and signaling. Proteomic profile of cell secretome wasn’t largely affected after immortalization converted by this piggyBac immortalization system. The secretome of iDFCs may be a good candidate of primary DFCs for regenerative medicine.
format	article
author	Lei Dou Yan Wu Qifang Yan Jinhua Wang Yan Zhang Ping Ji
author_facet	Lei Dou Yan Wu Qifang Yan Jinhua Wang Yan Zhang Ping Ji
author_sort	Lei Dou
title	Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis
title_short	Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis
title_full	Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis
title_fullStr	Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis
title_full_unstemmed	Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis
title_sort	secretome profiles of immortalized dental follicle cells using itraq-based proteomic analysis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/efb4b168972b430793766baae7ec6ebe
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Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis

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