IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES

The aim of this work was a simultaneous analysis of functional platelet activity using both standard optical aggregometry and flow cytometry, by means of measuring P-selectin and GP IIb-IIIa glycoprotein receptor contents on the surface of ADP-induced platelets. GP IIb-IIIa amounts and P-selectin ex...

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Autores principales:	O. V. Sirotkina, N. A. Bogan’kova, A. B. Laskovets, G. A. Kuharchik, L. B. Gaykovaya, T. V. Vavilova
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	p-selectin expression gp iib-iiia adp-induced platelet aggregation flow cytometry Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/efba3799c4b642f2acefa79e84740b49
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spelling	oai:doaj.org-article:efba3799c4b642f2acefa79e84740b492021-11-18T08:03:37ZIMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES1563-06252313-741X10.15789/1563-0625-2010-3-213-218https://doaj.org/article/efba3799c4b642f2acefa79e84740b492014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/93https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of this work was a simultaneous analysis of functional platelet activity using both standard optical aggregometry and flow cytometry, by means of measuring P-selectin and GP IIb-IIIa glycoprotein receptor contents on the surface of ADP-induced platelets. GP IIb-IIIa amounts and P-selectin expression on cell surface are significantly increased on platelets after activation with 10 mcM ADP, both in normal donors and in aspirin-treated patients. In clopidogrel-treated patients, the amounts of expressed P-selectin and GP IIb-IIIa did not change after ADP induction, thus suggesting absence of detectable ADP-induced platelet activation in this group. By means of Spearman criterion, a distinct correlation was found between the P-selectin expression, GP IIb-IIIa contents, and rates of standard ADP-induced aggregation. A combination of standard ADP-induced aggregation/activation of platelets with flow cytometry using FITC or PE-labeled antibodies to P-selectin and GP IIb-IIIa, allowed us to apply immunological approaches to analysis of platelet hemostasis, to propose novel laboratory criteria for evaluation of platelet hyperaggregation, and to detect differential effects of clopidogrel and aspirin.O. V. SirotkinaN. A. Bogan’kovaA. B. LaskovetsG. A. KuharchikL. B. GaykovayaT. V. VavilovaSPb RAACIarticlep-selectin expressiongp iib-iiiaadp-induced platelet aggregationflow cytometryImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 12, Iss 3, Pp 213-218 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	p-selectin expression gp iib-iiia adp-induced platelet aggregation flow cytometry Immunologic diseases. Allergy RC581-607
spellingShingle	p-selectin expression gp iib-iiia adp-induced platelet aggregation flow cytometry Immunologic diseases. Allergy RC581-607 O. V. Sirotkina N. A. Bogan’kova A. B. Laskovets G. A. Kuharchik L. B. Gaykovaya T. V. Vavilova IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES
description	The aim of this work was a simultaneous analysis of functional platelet activity using both standard optical aggregometry and flow cytometry, by means of measuring P-selectin and GP IIb-IIIa glycoprotein receptor contents on the surface of ADP-induced platelets. GP IIb-IIIa amounts and P-selectin expression on cell surface are significantly increased on platelets after activation with 10 mcM ADP, both in normal donors and in aspirin-treated patients. In clopidogrel-treated patients, the amounts of expressed P-selectin and GP IIb-IIIa did not change after ADP induction, thus suggesting absence of detectable ADP-induced platelet activation in this group. By means of Spearman criterion, a distinct correlation was found between the P-selectin expression, GP IIb-IIIa contents, and rates of standard ADP-induced aggregation. A combination of standard ADP-induced aggregation/activation of platelets with flow cytometry using FITC or PE-labeled antibodies to P-selectin and GP IIb-IIIa, allowed us to apply immunological approaches to analysis of platelet hemostasis, to propose novel laboratory criteria for evaluation of platelet hyperaggregation, and to detect differential effects of clopidogrel and aspirin.
format	article
author	O. V. Sirotkina N. A. Bogan’kova A. B. Laskovets G. A. Kuharchik L. B. Gaykovaya T. V. Vavilova
author_facet	O. V. Sirotkina N. A. Bogan’kova A. B. Laskovets G. A. Kuharchik L. B. Gaykovaya T. V. Vavilova
author_sort	O. V. Sirotkina
title	IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES
title_short	IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES
title_full	IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES
title_fullStr	IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES
title_full_unstemmed	IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES
title_sort	immunological methods for evaluation of platelet functions in the patients with cardiovascular diseases
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/efba3799c4b642f2acefa79e84740b49
work_keys_str_mv	AT ovsirotkina immunologicalmethodsforevaluationofplateletfunctionsinthepatientswithcardiovasculardiseases AT nabogankova immunologicalmethodsforevaluationofplateletfunctionsinthepatientswithcardiovasculardiseases AT ablaskovets immunologicalmethodsforevaluationofplateletfunctionsinthepatientswithcardiovasculardiseases AT gakuharchik immunologicalmethodsforevaluationofplateletfunctionsinthepatientswithcardiovasculardiseases AT lbgaykovaya immunologicalmethodsforevaluationofplateletfunctionsinthepatientswithcardiovasculardiseases AT tvvavilova immunologicalmethodsforevaluationofplateletfunctionsinthepatientswithcardiovasculardiseases
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IMMUNOLOGICAL METHODS FOR EVALUATION OF PLATELET FUNCTIONS IN THE PATIENTS WITH CARDIOVASCULAR DISEASES

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