Optogenetic axon guidance in embryonic zebrafish

Summary: Axons form the long-range connections of biological neuronal networks, which are built through the developmental process of axon guidance. Here, we describe a protocol to precisely and non-invasively control axonal growth trajectories in live zebrafish embryos using focal light activation o...

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Autores principales:	James M. Harris, Andy Yu-Der Wang, Paola Arlotta
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Developmental biology Microscopy Model Organisms Molecular Biology Neuroscience Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/efbc9f2949314fbc99395cc2c2f11438
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spelling	oai:doaj.org-article:efbc9f2949314fbc99395cc2c2f114382021-11-18T04:51:39ZOptogenetic axon guidance in embryonic zebrafish2666-166710.1016/j.xpro.2021.100947https://doaj.org/article/efbc9f2949314fbc99395cc2c2f114382021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006535https://doaj.org/toc/2666-1667Summary: Axons form the long-range connections of biological neuronal networks, which are built through the developmental process of axon guidance. Here, we describe a protocol to precisely and non-invasively control axonal growth trajectories in live zebrafish embryos using focal light activation of a photoactivatable Rac1. We outline techniques for photostimulation, time-lapse imaging, and immunohistochemistry. These approaches enable engineering of long-range axonal circuitry or repair of defective circuits in living zebrafish, despite a milieu of competing endogenous signals and repulsive barriers.For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).James M. HarrisAndy Yu-Der WangPaola ArlottaElsevierarticleDevelopmental biologyMicroscopyModel OrganismsMolecular BiologyNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100947- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Developmental biology Microscopy Model Organisms Molecular Biology Neuroscience Science (General) Q1-390
spellingShingle	Developmental biology Microscopy Model Organisms Molecular Biology Neuroscience Science (General) Q1-390 James M. Harris Andy Yu-Der Wang Paola Arlotta Optogenetic axon guidance in embryonic zebrafish
description	Summary: Axons form the long-range connections of biological neuronal networks, which are built through the developmental process of axon guidance. Here, we describe a protocol to precisely and non-invasively control axonal growth trajectories in live zebrafish embryos using focal light activation of a photoactivatable Rac1. We outline techniques for photostimulation, time-lapse imaging, and immunohistochemistry. These approaches enable engineering of long-range axonal circuitry or repair of defective circuits in living zebrafish, despite a milieu of competing endogenous signals and repulsive barriers.For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).
format	article
author	James M. Harris Andy Yu-Der Wang Paola Arlotta
author_facet	James M. Harris Andy Yu-Der Wang Paola Arlotta
author_sort	James M. Harris
title	Optogenetic axon guidance in embryonic zebrafish
title_short	Optogenetic axon guidance in embryonic zebrafish
title_full	Optogenetic axon guidance in embryonic zebrafish
title_fullStr	Optogenetic axon guidance in embryonic zebrafish
title_full_unstemmed	Optogenetic axon guidance in embryonic zebrafish
title_sort	optogenetic axon guidance in embryonic zebrafish
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/efbc9f2949314fbc99395cc2c2f11438
work_keys_str_mv	AT jamesmharris optogeneticaxonguidanceinembryoniczebrafish AT andyyuderwang optogeneticaxonguidanceinembryoniczebrafish AT paolaarlotta optogeneticaxonguidanceinembryoniczebrafish
_version_	1718425010614304768

Optogenetic axon guidance in embryonic zebrafish

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