YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1

Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH...

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Autores principales:	Luandie Ge, Yaqi Fan, Lin Fu, Mengjiao Guo, Panxia Cao, Chaojie Peng, Linke Wu, Lihua Han, Hong Wu
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/efc47392b07f41fda3d309417b408008
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spelling	oai:doaj.org-article:efc47392b07f41fda3d309417b4080082021-11-15T01:19:17ZYQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-11741-428810.1155/2021/4852406https://doaj.org/article/efc47392b07f41fda3d309417b4080082021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/4852406https://doaj.org/toc/1741-4288Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH), and creatine kinase (CK) levels and cardiomyocyte apoptosis were determined. LDH release, CK activity, caspase-3 activation, mRNA and protein ratio of Bax/Bcl-2, and miR-1 expression were significantly higher (p<0.01) in the H/R model of rat myocardial primary cells and H9c2 cells compared with the control group and was inhibited by YQHX treatment (p<0.01 or p<0.05). We also found that miR-1 overexpression could enhance apoptosis in cardiomyocytes, whereas apoptosis could be reduced by YQHX treatment (p<0.01). In conclusion, YQHX alleviates H/R-induced cardiomyocyte apoptosis by inhibiting miR-1 expression, suggesting the potential of YQHX in preventing MIRI.Luandie GeYaqi FanLin FuMengjiao GuoPanxia CaoChaojie PengLinke WuLihua HanHong WuHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Luandie Ge Yaqi Fan Lin Fu Mengjiao Guo Panxia Cao Chaojie Peng Linke Wu Lihua Han Hong Wu YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1
description	Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH), and creatine kinase (CK) levels and cardiomyocyte apoptosis were determined. LDH release, CK activity, caspase-3 activation, mRNA and protein ratio of Bax/Bcl-2, and miR-1 expression were significantly higher (p<0.01) in the H/R model of rat myocardial primary cells and H9c2 cells compared with the control group and was inhibited by YQHX treatment (p<0.01 or p<0.05). We also found that miR-1 overexpression could enhance apoptosis in cardiomyocytes, whereas apoptosis could be reduced by YQHX treatment (p<0.01). In conclusion, YQHX alleviates H/R-induced cardiomyocyte apoptosis by inhibiting miR-1 expression, suggesting the potential of YQHX in preventing MIRI.
format	article
author	Luandie Ge Yaqi Fan Lin Fu Mengjiao Guo Panxia Cao Chaojie Peng Linke Wu Lihua Han Hong Wu
author_facet	Luandie Ge Yaqi Fan Lin Fu Mengjiao Guo Panxia Cao Chaojie Peng Linke Wu Lihua Han Hong Wu
author_sort	Luandie Ge
title	YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1
title_short	YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1
title_full	YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1
title_fullStr	YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1
title_full_unstemmed	YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1
title_sort	yqhx alleviates h/r-induced cardiomyocyte apoptosis by downregulating mir-1
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/efc47392b07f41fda3d309417b408008
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YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1

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