<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents
Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and carotenoids, can avoid or treat oxidation-associated conditions and diseases like inflammation or cancer. This study aimed to assess the bioactive potential of lipidic extracts obtained from <i>Gloeothece...
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2021
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oai:doaj.org-article:efde6215f35e457e8b18aacab84369822021-11-25T18:12:53Z<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents10.3390/md191106231660-3397https://doaj.org/article/efde6215f35e457e8b18aacab84369822021-11-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/623https://doaj.org/toc/1660-3397Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and carotenoids, can avoid or treat oxidation-associated conditions and diseases like inflammation or cancer. This study aimed to assess the bioactive potential of lipidic extracts obtained from <i>Gloeothece</i> sp.–using Generally Recognized as Safe (GRAS) solvents like ethanol, acetone, hexane:isopropanol (3:2) (HI) and ethyl lactate. The bioactive potential of extracts was assessed in terms of antioxidant (ABTS<sup>•+</sup>, DPPH<sup>•</sup>, <sup>•</sup>NO and O<sub>2</sub><sup>•</sup>assays), anti-inflammatory (HRBC membrane stabilization and Cox-2 screening assay), and antitumor capacity (death by TUNEL, and anti-proliferative by BrdU incorporation assay in AGS cancer cells); while its composition was characterized in terms of carotenoids and fatty acids, by HPLC-DAD and GC-FID methods, respectively. Results revealed a chemopreventive potential of the HI extract owing to its ability to: (I) scavenge <sup>-</sup>NO<sup>•</sup> radical (IC<sub>50</sub>, 1258 ± 0.353 µg·mL<sup>−1</sup>); (II) inhibit 50% of COX-2 expression at 130.2 ± 7.4 µg·mL<sup>−1</sup>; (III) protect 61.6 ± 9.2% of lysosomes from heat damage, and (IV) induce AGS cell death by 4.2-fold and avoid its proliferation up to 40% in a concentration of 23.2 ± 1.9 µg·mL<sup>−1</sup>. Hence, <i>Gloeothece</i> sp. extracts, namely HI, were revealed to have the potential to be used for nutraceutical purposes.Helena M. AmaroRita BarrosTânia TavaresRaquel AlmeidaIsabel Sousa PintoFrancisco Xavier MalcataAna Catarina GuedesMDPI AGarticleluteinβ-carotenelinolenic acidlinoleic acidlipidic compoundscarotenoidsBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 623, p 623 (2021) |
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EN |
| topic |
lutein β-carotene linolenic acid linoleic acid lipidic compounds carotenoids Biology (General) QH301-705.5 |
| spellingShingle |
lutein β-carotene linolenic acid linoleic acid lipidic compounds carotenoids Biology (General) QH301-705.5 Helena M. Amaro Rita Barros Tânia Tavares Raquel Almeida Isabel Sousa Pinto Francisco Xavier Malcata Ana Catarina Guedes <i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents |
| description |
Bioactive lipidic compounds of microalgae, such as polyunsaturated fatty acids (PUFA) and carotenoids, can avoid or treat oxidation-associated conditions and diseases like inflammation or cancer. This study aimed to assess the bioactive potential of lipidic extracts obtained from <i>Gloeothece</i> sp.–using Generally Recognized as Safe (GRAS) solvents like ethanol, acetone, hexane:isopropanol (3:2) (HI) and ethyl lactate. The bioactive potential of extracts was assessed in terms of antioxidant (ABTS<sup>•+</sup>, DPPH<sup>•</sup>, <sup>•</sup>NO and O<sub>2</sub><sup>•</sup>assays), anti-inflammatory (HRBC membrane stabilization and Cox-2 screening assay), and antitumor capacity (death by TUNEL, and anti-proliferative by BrdU incorporation assay in AGS cancer cells); while its composition was characterized in terms of carotenoids and fatty acids, by HPLC-DAD and GC-FID methods, respectively. Results revealed a chemopreventive potential of the HI extract owing to its ability to: (I) scavenge <sup>-</sup>NO<sup>•</sup> radical (IC<sub>50</sub>, 1258 ± 0.353 µg·mL<sup>−1</sup>); (II) inhibit 50% of COX-2 expression at 130.2 ± 7.4 µg·mL<sup>−1</sup>; (III) protect 61.6 ± 9.2% of lysosomes from heat damage, and (IV) induce AGS cell death by 4.2-fold and avoid its proliferation up to 40% in a concentration of 23.2 ± 1.9 µg·mL<sup>−1</sup>. Hence, <i>Gloeothece</i> sp. extracts, namely HI, were revealed to have the potential to be used for nutraceutical purposes. |
| format |
article |
| author |
Helena M. Amaro Rita Barros Tânia Tavares Raquel Almeida Isabel Sousa Pinto Francisco Xavier Malcata Ana Catarina Guedes |
| author_facet |
Helena M. Amaro Rita Barros Tânia Tavares Raquel Almeida Isabel Sousa Pinto Francisco Xavier Malcata Ana Catarina Guedes |
| author_sort |
Helena M. Amaro |
| title |
<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents |
| title_short |
<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents |
| title_full |
<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents |
| title_fullStr |
<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents |
| title_full_unstemmed |
<i>Gloeothece</i> sp.—Exploiting a New Source of Antioxidant, Anti-Inflammatory, and Antitumor Agents |
| title_sort |
<i>gloeothece</i> sp.—exploiting a new source of antioxidant, anti-inflammatory, and antitumor agents |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/efde6215f35e457e8b18aacab8436982 |
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