Development of a PCR-based dot blot assay for the detection of fowl adenovirus

ABSTRACT: Group-I Fowl adenovirus (FAdV) is still widespread in China's chicken farms, leading to huge economic losses. The traditional PCR method, which can detect all serotypes at the same time, is not sensitive enough to obtain accurate results, especially in some samples containing only a l...

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Autores principales:	Lidan Hou, Qi Su, Yawen Zhang, Dan Liu, Yaqing Mao, Peng Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	dot blot fowl adenovirus detection PCR serotypes Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/efe1acc515c3439b900914e2b61a6153
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spelling	oai:doaj.org-article:efe1acc515c3439b900914e2b61a61532021-11-24T04:23:09ZDevelopment of a PCR-based dot blot assay for the detection of fowl adenovirus0032-579110.1016/j.psj.2021.101540https://doaj.org/article/efe1acc515c3439b900914e2b61a61532022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005629https://doaj.org/toc/0032-5791ABSTRACT: Group-I Fowl adenovirus (FAdV) is still widespread in China's chicken farms, leading to huge economic losses. The traditional PCR method, which can detect all serotypes at the same time, is not sensitive enough to obtain accurate results, especially in some samples containing only a low titer of virus, such as contaminated live vaccine. In order to solve this problem, this study developed a dot blot assay based on the above PCR method. A total of 6 probes targeting the conserved region of FAdV were designed and systematically optimized through sensitivity, accuracy, and stability analyses. Results showed that it is not only suitable for 12 serotypes, but also effectively improve the sensitivity, which increased more than 100 times in comparison with PCR assay. Moreover, this sensitivity was increased 100 times when detecting contaminated live vaccine samples, showing the great prospect of this method in daily monitoring.Lidan HouQi SuYawen ZhangDan LiuYaqing MaoPeng ZhaoElsevierarticledot blotfowl adenovirusdetectionPCRserotypesAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101540- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	dot blot fowl adenovirus detection PCR serotypes Animal culture SF1-1100
spellingShingle	dot blot fowl adenovirus detection PCR serotypes Animal culture SF1-1100 Lidan Hou Qi Su Yawen Zhang Dan Liu Yaqing Mao Peng Zhao Development of a PCR-based dot blot assay for the detection of fowl adenovirus
description	ABSTRACT: Group-I Fowl adenovirus (FAdV) is still widespread in China's chicken farms, leading to huge economic losses. The traditional PCR method, which can detect all serotypes at the same time, is not sensitive enough to obtain accurate results, especially in some samples containing only a low titer of virus, such as contaminated live vaccine. In order to solve this problem, this study developed a dot blot assay based on the above PCR method. A total of 6 probes targeting the conserved region of FAdV were designed and systematically optimized through sensitivity, accuracy, and stability analyses. Results showed that it is not only suitable for 12 serotypes, but also effectively improve the sensitivity, which increased more than 100 times in comparison with PCR assay. Moreover, this sensitivity was increased 100 times when detecting contaminated live vaccine samples, showing the great prospect of this method in daily monitoring.
format	article
author	Lidan Hou Qi Su Yawen Zhang Dan Liu Yaqing Mao Peng Zhao
author_facet	Lidan Hou Qi Su Yawen Zhang Dan Liu Yaqing Mao Peng Zhao
author_sort	Lidan Hou
title	Development of a PCR-based dot blot assay for the detection of fowl adenovirus
title_short	Development of a PCR-based dot blot assay for the detection of fowl adenovirus
title_full	Development of a PCR-based dot blot assay for the detection of fowl adenovirus
title_fullStr	Development of a PCR-based dot blot assay for the detection of fowl adenovirus
title_full_unstemmed	Development of a PCR-based dot blot assay for the detection of fowl adenovirus
title_sort	development of a pcr-based dot blot assay for the detection of fowl adenovirus
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/efe1acc515c3439b900914e2b61a6153
work_keys_str_mv	AT lidanhou developmentofapcrbaseddotblotassayforthedetectionoffowladenovirus AT qisu developmentofapcrbaseddotblotassayforthedetectionoffowladenovirus AT yawenzhang developmentofapcrbaseddotblotassayforthedetectionoffowladenovirus AT danliu developmentofapcrbaseddotblotassayforthedetectionoffowladenovirus AT yaqingmao developmentofapcrbaseddotblotassayforthedetectionoffowladenovirus AT pengzhao developmentofapcrbaseddotblotassayforthedetectionoffowladenovirus
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Development of a PCR-based dot blot assay for the detection of fowl adenovirus

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