The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea

ABSTRACT Precise control of the cell cycle is central to the physiology of all cells. In prior work we demonstrated that archaeal cells maintain a constant size; however, the regulatory mechanisms underlying the cell cycle remain unexplored in this domain of life. Here, we use genetics, functional g...

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Autores principales:	Cynthia L. Darnell, Jenny Zheng, Sean Wilson, Ryan M. Bertoli, Alexandre W. Bisson-Filho, Ethan C. Garner, Amy K. Schmid
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	archaea cell division gene regulation transcription factors video microscopy Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/efe91ec2cd5b4204ab3646133fe6130f
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spelling	oai:doaj.org-article:efe91ec2cd5b4204ab3646133fe6130f2021-11-15T15:56:43ZThe Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea10.1128/mBio.01007-202150-7511https://doaj.org/article/efe91ec2cd5b4204ab3646133fe6130f2020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01007-20https://doaj.org/toc/2150-7511ABSTRACT Precise control of the cell cycle is central to the physiology of all cells. In prior work we demonstrated that archaeal cells maintain a constant size; however, the regulatory mechanisms underlying the cell cycle remain unexplored in this domain of life. Here, we use genetics, functional genomics, and quantitative imaging to identify and characterize the novel CdrSL gene regulatory network in a model species of archaea. We demonstrate the central role of these ribbon-helix-helix family transcription factors in the regulation of cell division through specific transcriptional control of the gene encoding FtsZ2, a putative tubulin homolog. Using time-lapse fluorescence microscopy in live cells cultivated in microfluidics devices, we further demonstrate that FtsZ2 is required for cell division but not elongation. The cdrS-ftsZ2 locus is highly conserved throughout the archaeal domain, and the central function of CdrS in regulating cell division is conserved across hypersaline adapted archaea. We propose that the CdrSL-FtsZ2 transcriptional network coordinates cell division timing with cell growth in archaea. IMPORTANCE Healthy cell growth and division are critical for individual organism survival and species long-term viability. However, it remains unknown how cells of the domain Archaea maintain a healthy cell cycle. Understanding the archaeal cell cycle is of paramount evolutionary importance given that an archaeal cell was the host of the endosymbiotic event that gave rise to eukaryotes. Here, we identify and characterize novel molecular players needed for regulating cell division in archaea. These molecules dictate the timing of cell septation but are dispensable for growth between divisions. Timing is accomplished through transcriptional control of the cell division ring. Our results shed light on mechanisms underlying the archaeal cell cycle, which has thus far remained elusive.Cynthia L. DarnellJenny ZhengSean WilsonRyan M. BertoliAlexandre W. Bisson-FilhoEthan C. GarnerAmy K. SchmidAmerican Society for Microbiologyarticlearchaeacell divisiongene regulationtranscription factorsvideo microscopyMicrobiologyQR1-502ENmBio, Vol 11, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	archaea cell division gene regulation transcription factors video microscopy Microbiology QR1-502
spellingShingle	archaea cell division gene regulation transcription factors video microscopy Microbiology QR1-502 Cynthia L. Darnell Jenny Zheng Sean Wilson Ryan M. Bertoli Alexandre W. Bisson-Filho Ethan C. Garner Amy K. Schmid The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea
description	ABSTRACT Precise control of the cell cycle is central to the physiology of all cells. In prior work we demonstrated that archaeal cells maintain a constant size; however, the regulatory mechanisms underlying the cell cycle remain unexplored in this domain of life. Here, we use genetics, functional genomics, and quantitative imaging to identify and characterize the novel CdrSL gene regulatory network in a model species of archaea. We demonstrate the central role of these ribbon-helix-helix family transcription factors in the regulation of cell division through specific transcriptional control of the gene encoding FtsZ2, a putative tubulin homolog. Using time-lapse fluorescence microscopy in live cells cultivated in microfluidics devices, we further demonstrate that FtsZ2 is required for cell division but not elongation. The cdrS-ftsZ2 locus is highly conserved throughout the archaeal domain, and the central function of CdrS in regulating cell division is conserved across hypersaline adapted archaea. We propose that the CdrSL-FtsZ2 transcriptional network coordinates cell division timing with cell growth in archaea. IMPORTANCE Healthy cell growth and division are critical for individual organism survival and species long-term viability. However, it remains unknown how cells of the domain Archaea maintain a healthy cell cycle. Understanding the archaeal cell cycle is of paramount evolutionary importance given that an archaeal cell was the host of the endosymbiotic event that gave rise to eukaryotes. Here, we identify and characterize novel molecular players needed for regulating cell division in archaea. These molecules dictate the timing of cell septation but are dispensable for growth between divisions. Timing is accomplished through transcriptional control of the cell division ring. Our results shed light on mechanisms underlying the archaeal cell cycle, which has thus far remained elusive.
format	article
author	Cynthia L. Darnell Jenny Zheng Sean Wilson Ryan M. Bertoli Alexandre W. Bisson-Filho Ethan C. Garner Amy K. Schmid
author_facet	Cynthia L. Darnell Jenny Zheng Sean Wilson Ryan M. Bertoli Alexandre W. Bisson-Filho Ethan C. Garner Amy K. Schmid
author_sort	Cynthia L. Darnell
title	The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea
title_short	The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea
title_full	The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea
title_fullStr	The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea
title_full_unstemmed	The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea
title_sort	ribbon-helix-helix domain protein cdrs regulates the tubulin homolog <italic toggle="yes">ftsz2</italic> to control cell division in archaea
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/efe91ec2cd5b4204ab3646133fe6130f
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The Ribbon-Helix-Helix Domain Protein CdrS Regulates the Tubulin Homolog <italic toggle="yes">ftsZ2</italic> To Control Cell Division in Archaea

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