A catalogue of biochemically diverse CRISPR-Cas9 orthologs

A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nuclea...

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Autores principales:	Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
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spelling	oai:doaj.org-article:eff8b92a7b8248269794dd870fd5600c2021-12-02T17:33:12ZA catalogue of biochemically diverse CRISPR-Cas9 orthologs10.1038/s41467-020-19344-12041-1723https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19344-1https://doaj.org/toc/2041-1723A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nucleases.Giedrius GasiunasJoshua K. YoungTautvydas KarvelisDarius KazlauskasTomas UrbaitisMonika JasnauskaiteMantvyda M. GrusyteSushmitha PaulrajPo-Hao WangZhenglin HouShane K. DooleyMark CiganClara AlarconN. Doane ChilcoatGreta BigelyteJennifer L. CurcuruMegumu MabuchiZhiyi SunRyan T. FuchsEzra SchildkrautPeter R. WeigeleWilliam E. JackG. Brett RobbČeslovas VenclovasVirginijus SiksnysNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Giedrius Gasiunas Joshua K. Young Tautvydas Karvelis Darius Kazlauskas Tomas Urbaitis Monika Jasnauskaite Mantvyda M. Grusyte Sushmitha Paulraj Po-Hao Wang Zhenglin Hou Shane K. Dooley Mark Cigan Clara Alarcon N. Doane Chilcoat Greta Bigelyte Jennifer L. Curcuru Megumu Mabuchi Zhiyi Sun Ryan T. Fuchs Ezra Schildkraut Peter R. Weigele William E. Jack G. Brett Robb Česlovas Venclovas Virginijus Siksnys A catalogue of biochemically diverse CRISPR-Cas9 orthologs
description	A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nucleases.
format	article
author	Giedrius Gasiunas Joshua K. Young Tautvydas Karvelis Darius Kazlauskas Tomas Urbaitis Monika Jasnauskaite Mantvyda M. Grusyte Sushmitha Paulraj Po-Hao Wang Zhenglin Hou Shane K. Dooley Mark Cigan Clara Alarcon N. Doane Chilcoat Greta Bigelyte Jennifer L. Curcuru Megumu Mabuchi Zhiyi Sun Ryan T. Fuchs Ezra Schildkraut Peter R. Weigele William E. Jack G. Brett Robb Česlovas Venclovas Virginijus Siksnys
author_facet	Giedrius Gasiunas Joshua K. Young Tautvydas Karvelis Darius Kazlauskas Tomas Urbaitis Monika Jasnauskaite Mantvyda M. Grusyte Sushmitha Paulraj Po-Hao Wang Zhenglin Hou Shane K. Dooley Mark Cigan Clara Alarcon N. Doane Chilcoat Greta Bigelyte Jennifer L. Curcuru Megumu Mabuchi Zhiyi Sun Ryan T. Fuchs Ezra Schildkraut Peter R. Weigele William E. Jack G. Brett Robb Česlovas Venclovas Virginijus Siksnys
author_sort	Giedrius Gasiunas
title	A catalogue of biochemically diverse CRISPR-Cas9 orthologs
title_short	A catalogue of biochemically diverse CRISPR-Cas9 orthologs
title_full	A catalogue of biochemically diverse CRISPR-Cas9 orthologs
title_fullStr	A catalogue of biochemically diverse CRISPR-Cas9 orthologs
title_full_unstemmed	A catalogue of biochemically diverse CRISPR-Cas9 orthologs
title_sort	catalogue of biochemically diverse crispr-cas9 orthologs
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
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A catalogue of biochemically diverse CRISPR-Cas9 orthologs

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