Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy
Abstract Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry (VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the cellular basis of GMV changes has remained largely unclear. We correlated changes in GMV with cellular metrics by imaging...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/efff63cb8c634002b9771ee8ae1f390e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:efff63cb8c634002b9771ee8ae1f390e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:efff63cb8c634002b9771ee8ae1f390e2021-12-02T10:54:23ZCellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy10.1038/s41598-021-83491-82045-2322https://doaj.org/article/efff63cb8c634002b9771ee8ae1f390e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83491-8https://doaj.org/toc/2045-2322Abstract Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry (VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the cellular basis of GMV changes has remained largely unclear. We correlated changes in GMV with cellular metrics by imaging mice with MRI and two-photon in vivo microscopy at three time points within 12 weeks, taking advantage of age-dependent changes in brain structure. Imaging fluorescent cell nuclei allowed inferences on (i) physical tissue volume as determined from reference spaces outlined by nuclei, (ii) cell density, (iii) the extent of cell clustering, and (iv) the volume of cell nuclei. Our data indicate that physical tissue volume alterations only account for 13.0% of the variance in GMV change. However, when including comprehensive measurements of nucleus volume and cell density, 35.6% of the GMV variance could be explained, highlighting the influence of distinct cellular mechanisms on VBM results.Livia AsanClaudia Falfán-MelgozaCarlo A. BerettaMarkus SackLei ZhengWolfgang Weber-FahrThomas KunerJohannes KnabbeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-20 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Livia Asan Claudia Falfán-Melgoza Carlo A. Beretta Markus Sack Lei Zheng Wolfgang Weber-Fahr Thomas Kuner Johannes Knabbe Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| description |
Abstract Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry (VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the cellular basis of GMV changes has remained largely unclear. We correlated changes in GMV with cellular metrics by imaging mice with MRI and two-photon in vivo microscopy at three time points within 12 weeks, taking advantage of age-dependent changes in brain structure. Imaging fluorescent cell nuclei allowed inferences on (i) physical tissue volume as determined from reference spaces outlined by nuclei, (ii) cell density, (iii) the extent of cell clustering, and (iv) the volume of cell nuclei. Our data indicate that physical tissue volume alterations only account for 13.0% of the variance in GMV change. However, when including comprehensive measurements of nucleus volume and cell density, 35.6% of the GMV variance could be explained, highlighting the influence of distinct cellular mechanisms on VBM results. |
| format |
article |
| author |
Livia Asan Claudia Falfán-Melgoza Carlo A. Beretta Markus Sack Lei Zheng Wolfgang Weber-Fahr Thomas Kuner Johannes Knabbe |
| author_facet |
Livia Asan Claudia Falfán-Melgoza Carlo A. Beretta Markus Sack Lei Zheng Wolfgang Weber-Fahr Thomas Kuner Johannes Knabbe |
| author_sort |
Livia Asan |
| title |
Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_short |
Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_full |
Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_fullStr |
Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_full_unstemmed |
Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_sort |
cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/efff63cb8c634002b9771ee8ae1f390e |
| work_keys_str_mv |
AT liviaasan cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT claudiafalfanmelgoza cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT carloaberetta cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT markussack cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT leizheng cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT wolfgangweberfahr cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT thomaskuner cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy AT johannesknabbe cellularcorrelatesofgraymattervolumechangesinmagneticresonancemorphometryidentifiedbytwophotonmicroscopy |
| _version_ |
1718396504831426560 |