Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant

Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an...

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Autores principales:	Yong Zi Tan, Sriram Aiyer, Mario Mietzsch, Joshua A. Hull, Robert McKenna, Joshua Grieger, R. Jude Samulski, Timothy S. Baker, Mavis Agbandje-McKenna, Dmitry Lyumkis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f000b02747a14526ae63d8ed17d8476c
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spelling	oai:doaj.org-article:f000b02747a14526ae63d8ed17d8476c2021-12-02T16:50:06ZSub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant10.1038/s41467-018-06076-62041-1723https://doaj.org/article/f000b02747a14526ae63d8ed17d8476c2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06076-6https://doaj.org/toc/2041-1723Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an adeno-associated virus serotype 2 variant.Yong Zi TanSriram AiyerMario MietzschJoshua A. HullRobert McKennaJoshua GriegerR. Jude SamulskiTimothy S. BakerMavis Agbandje-McKennaDmitry LyumkisNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yong Zi Tan Sriram Aiyer Mario Mietzsch Joshua A. Hull Robert McKenna Joshua Grieger R. Jude Samulski Timothy S. Baker Mavis Agbandje-McKenna Dmitry Lyumkis Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
description	Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an adeno-associated virus serotype 2 variant.
format	article
author	Yong Zi Tan Sriram Aiyer Mario Mietzsch Joshua A. Hull Robert McKenna Joshua Grieger R. Jude Samulski Timothy S. Baker Mavis Agbandje-McKenna Dmitry Lyumkis
author_facet	Yong Zi Tan Sriram Aiyer Mario Mietzsch Joshua A. Hull Robert McKenna Joshua Grieger R. Jude Samulski Timothy S. Baker Mavis Agbandje-McKenna Dmitry Lyumkis
author_sort	Yong Zi Tan
title	Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
title_short	Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
title_full	Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
title_fullStr	Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
title_full_unstemmed	Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
title_sort	sub-2 å ewald curvature corrected structure of an aav2 capsid variant
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f000b02747a14526ae63d8ed17d8476c
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Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant

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