Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections

Katsuji Kiuchi Kiuchi Eye Clinic, Joto-ku, Osaka, Japan Purpose: To present the Löffler’s alkaline methylene blue technique of staining eye discharges in eyes with anterior segment infections.Method: The Löffler’s alkaline methylene blue staining method is a s...

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Autor principal:	Kiuchi K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	alkaline methylene blue supravaital staining discharge anterior segment infections Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/f0012b94aadf484a8696b027c7c1636a
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spelling	oai:doaj.org-article:f0012b94aadf484a8696b027c7c1636a2021-12-02T01:01:37ZRapid alkaline methylene blue supravital staining for assessment of anterior segment infections1177-5483https://doaj.org/article/f0012b94aadf484a8696b027c7c1636a2016-10-01T00:00:00Zhttps://www.dovepress.com/rapid-alkaline-methylene-blue-supravital-staining-for-assessment-of-an-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Katsuji Kiuchi Kiuchi Eye Clinic, Joto-ku, Osaka, Japan Purpose: To present the Löffler’s alkaline methylene blue technique of staining eye discharges in eyes with anterior segment infections.Method: The Löffler’s alkaline methylene blue staining method is a simple staining technique that can be used to differentiate bacterial, viral, and fungal infections. It is a cationic dye that stains cells blue because the positively charged dye is attracted to negatively charged particles such as polyphosphates, DNAs, and RNAs. Specimens collected from patients by swabbing are smeared onto microscope slides and the methylene blue solution is dropped on the slide. The slide is covered with a glass cover slip and examined under a microscope. The entire time from the collection to the viewing is about 30 seconds.Results: Histopathological images of the conjunctival epithelial cells and neutrophils in eye discharges were dyed blue and the nuclei were stained more intensely blue. Bacterial infections consisted mainly of neutrophils, and viral infections consisted mainly of lymphocytes.Conclusions: Löffler’s alkaline methylene blue staining can be done in about 30 seconds for diagnosis. Even though this is a one color stain, it is possible to infer the cause of the infection by detection of the absence of bacteria and/or fungi in context of the differential distribution of neutrophils and lymphocytes. Keywords: alkaline methylene blue, supravital staining, discharge, anterior segment infectionsKiuchi KDove Medical Pressarticlealkaline methylene bluesupravaital stainingdischargeanterior segment infectionsOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 10, Pp 1971-1975 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	alkaline methylene blue supravaital staining discharge anterior segment infections Ophthalmology RE1-994
spellingShingle	alkaline methylene blue supravaital staining discharge anterior segment infections Ophthalmology RE1-994 Kiuchi K Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
description	Katsuji Kiuchi Kiuchi Eye Clinic, Joto-ku, Osaka, Japan Purpose: To present the Löffler’s alkaline methylene blue technique of staining eye discharges in eyes with anterior segment infections.Method: The Löffler’s alkaline methylene blue staining method is a simple staining technique that can be used to differentiate bacterial, viral, and fungal infections. It is a cationic dye that stains cells blue because the positively charged dye is attracted to negatively charged particles such as polyphosphates, DNAs, and RNAs. Specimens collected from patients by swabbing are smeared onto microscope slides and the methylene blue solution is dropped on the slide. The slide is covered with a glass cover slip and examined under a microscope. The entire time from the collection to the viewing is about 30 seconds.Results: Histopathological images of the conjunctival epithelial cells and neutrophils in eye discharges were dyed blue and the nuclei were stained more intensely blue. Bacterial infections consisted mainly of neutrophils, and viral infections consisted mainly of lymphocytes.Conclusions: Löffler’s alkaline methylene blue staining can be done in about 30 seconds for diagnosis. Even though this is a one color stain, it is possible to infer the cause of the infection by detection of the absence of bacteria and/or fungi in context of the differential distribution of neutrophils and lymphocytes. Keywords: alkaline methylene blue, supravital staining, discharge, anterior segment infections
format	article
author	Kiuchi K
author_facet	Kiuchi K
author_sort	Kiuchi K
title	Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
title_short	Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
title_full	Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
title_fullStr	Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
title_full_unstemmed	Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
title_sort	rapid alkaline methylene blue supravital staining for assessment of anterior segment infections
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/f0012b94aadf484a8696b027c7c1636a
work_keys_str_mv	AT kiuchik rapidalkalinemethylenebluesupravitalstainingforassessmentofanteriorsegmentinfections
_version_	1718403412744208384

Rapid alkaline methylene blue supravital staining for assessment of anterior segment infections

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