Comparative evaluation of genotoxicity in tobacco users versus nontobacco users

Background: Many of the contents of cigarette smoke are genotoxic in nature, and consequently, cytogenetic injury seems to be a trustworthy biomarker for deciding the influence of exposure to chromosome damaging agents in smoke. The cytokinesis-block micronucleus assay (CBMN assay) has been proven t...

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Autores principales:	Arun Jacob Thomas, Bindu J Nair, Sajna Oommen, V Syamkumar, Resmi K Raman
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	genotoxicity micronuclei tobacco Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142
Acceso en línea:	https://doaj.org/article/f0056279502b4aec81d4be832aa4ac0c
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spelling	oai:doaj.org-article:f0056279502b4aec81d4be832aa4ac0c2021-11-19T12:15:51ZComparative evaluation of genotoxicity in tobacco users versus nontobacco users0975-740610.4103/jpbs.jpbs_60_21https://doaj.org/article/f0056279502b4aec81d4be832aa4ac0c2021-01-01T00:00:00Zhttp://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=6;spage=960;epage=964;aulast=Thomashttps://doaj.org/toc/0975-7406Background: Many of the contents of cigarette smoke are genotoxic in nature, and consequently, cytogenetic injury seems to be a trustworthy biomarker for deciding the influence of exposure to chromosome damaging agents in smoke. The cytokinesis-block micronucleus assay (CBMN assay) has been proven to be an effectual tool for the study of micronuclei (MN) that will help in estimating the genotoxicity in tobacco users alone which will further help in early cancer detection. Objective: The objective is to find out whether there is pronounced contrast in genotoxicity between tobacco users and nonusers by determining MN number in peripheral blood lymphocytes using CBMN assay. Methodology: MN frequency in peripheral blood lymphocytes was estimated in 5 ml of fresh blood obtained from sixty individuals using tobacco either smoking, chewing, or combination of both and also from thirty individuals with no habit of tobacco use. All were in the age group of 20–40 years. Results: There was a significant increase in genotoxicity in tobacco users when compared to that of nontobacco users. A positive correlation was also obtained between smoking index and MN frequency in the study. Conclusion: Approximation of frequency of MN by CBMN assay can be used to evaluate the genotoxicity present in blood and helps in identifying tobacco users who are at a high risk for the presence of cancer even before the appearance of clinical changes.Arun Jacob ThomasBindu J NairSajna OommenV SyamkumarResmi K RamanWolters Kluwer Medknow PublicationsarticlegenotoxicitymicronucleitobaccoPharmacy and materia medicaRS1-441Analytical chemistryQD71-142ENJournal of Pharmacy and Bioallied Sciences, Vol 13, Iss 6, Pp 960-964 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	genotoxicity micronuclei tobacco Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142
spellingShingle	genotoxicity micronuclei tobacco Pharmacy and materia medica RS1-441 Analytical chemistry QD71-142 Arun Jacob Thomas Bindu J Nair Sajna Oommen V Syamkumar Resmi K Raman Comparative evaluation of genotoxicity in tobacco users versus nontobacco users
description	Background: Many of the contents of cigarette smoke are genotoxic in nature, and consequently, cytogenetic injury seems to be a trustworthy biomarker for deciding the influence of exposure to chromosome damaging agents in smoke. The cytokinesis-block micronucleus assay (CBMN assay) has been proven to be an effectual tool for the study of micronuclei (MN) that will help in estimating the genotoxicity in tobacco users alone which will further help in early cancer detection. Objective: The objective is to find out whether there is pronounced contrast in genotoxicity between tobacco users and nonusers by determining MN number in peripheral blood lymphocytes using CBMN assay. Methodology: MN frequency in peripheral blood lymphocytes was estimated in 5 ml of fresh blood obtained from sixty individuals using tobacco either smoking, chewing, or combination of both and also from thirty individuals with no habit of tobacco use. All were in the age group of 20–40 years. Results: There was a significant increase in genotoxicity in tobacco users when compared to that of nontobacco users. A positive correlation was also obtained between smoking index and MN frequency in the study. Conclusion: Approximation of frequency of MN by CBMN assay can be used to evaluate the genotoxicity present in blood and helps in identifying tobacco users who are at a high risk for the presence of cancer even before the appearance of clinical changes.
format	article
author	Arun Jacob Thomas Bindu J Nair Sajna Oommen V Syamkumar Resmi K Raman
author_facet	Arun Jacob Thomas Bindu J Nair Sajna Oommen V Syamkumar Resmi K Raman
author_sort	Arun Jacob Thomas
title	Comparative evaluation of genotoxicity in tobacco users versus nontobacco users
title_short	Comparative evaluation of genotoxicity in tobacco users versus nontobacco users
title_full	Comparative evaluation of genotoxicity in tobacco users versus nontobacco users
title_fullStr	Comparative evaluation of genotoxicity in tobacco users versus nontobacco users
title_full_unstemmed	Comparative evaluation of genotoxicity in tobacco users versus nontobacco users
title_sort	comparative evaluation of genotoxicity in tobacco users versus nontobacco users
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/f0056279502b4aec81d4be832aa4ac0c
work_keys_str_mv	AT arunjacobthomas comparativeevaluationofgenotoxicityintobaccousersversusnontobaccousers AT bindujnair comparativeevaluationofgenotoxicityintobaccousersversusnontobaccousers AT sajnaoommen comparativeevaluationofgenotoxicityintobaccousersversusnontobaccousers AT vsyamkumar comparativeevaluationofgenotoxicityintobaccousersversusnontobaccousers AT resmikraman comparativeevaluationofgenotoxicityintobaccousersversusnontobaccousers
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Comparative evaluation of genotoxicity in tobacco users versus nontobacco users

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