Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.

In order to enrich the phylogenetic diversity represented in the available sequenced bacterial genomes and as part of an "Assembling the Tree of Life" project, we determined the genome sequence of Thermomicrobium roseum DSM 5159. T. roseum DSM 5159 is a red-pigmented, rod-shaped, Gram-nega...

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Autores principales:	Dongying Wu, Jason Raymond, Martin Wu, Sourav Chatterji, Qinghu Ren, Joel E Graham, Donald A Bryant, Frank Robb, Albert Colman, Luke J Tallon, Jonathan H Badger, Ramana Madupu, Naomi L Ward, Jonathan A Eisen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f00f2032cda24874b02937341995f6b2
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spelling	oai:doaj.org-article:f00f2032cda24874b02937341995f6b22021-11-25T06:17:44ZComplete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.1932-620310.1371/journal.pone.0004207https://doaj.org/article/f00f2032cda24874b02937341995f6b22009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19148287/?tool=EBIhttps://doaj.org/toc/1932-6203In order to enrich the phylogenetic diversity represented in the available sequenced bacterial genomes and as part of an "Assembling the Tree of Life" project, we determined the genome sequence of Thermomicrobium roseum DSM 5159. T. roseum DSM 5159 is a red-pigmented, rod-shaped, Gram-negative extreme thermophile isolated from a hot spring that possesses both an atypical cell wall composition and an unusual cell membrane that is composed entirely of long-chain 1,2-diols. Its genome is composed of two circular DNA elements, one of 2,006,217 bp (referred to as the chromosome) and one of 919,596 bp (referred to as the megaplasmid). Strikingly, though few standard housekeeping genes are found on the megaplasmid, it does encode a complete system for chemotaxis including both chemosensory components and an entire flagellar apparatus. This is the first known example of a complete flagellar system being encoded on a plasmid and suggests a straightforward means for lateral transfer of flagellum-based motility. Phylogenomic analyses support the recent rRNA-based analyses that led to T. roseum being removed from the phylum Thermomicrobia and assigned to the phylum Chloroflexi. Because T. roseum is a deep-branching member of this phylum, analysis of its genome provides insights into the evolution of the Chloroflexi. In addition, even though this species is not photosynthetic, analysis of the genome provides some insight into the origins of photosynthesis in the Chloroflexi. Metabolic pathway reconstructions and experimental studies revealed new aspects of the biology of this species. For example, we present evidence that T. roseum oxidizes CO aerobically, making it the first thermophile known to do so. In addition, we propose that glycosylation of its carotenoids plays a crucial role in the adaptation of the cell membrane to this bacterium's thermophilic lifestyle. Analyses of published metagenomic sequences from two hot springs similar to the one from which this strain was isolated, show that close relatives of T. roseum DSM 5159 are present but have some key differences from the strain sequenced.Dongying WuJason RaymondMartin WuSourav ChatterjiQinghu RenJoel E GrahamDonald A BryantFrank RobbAlbert ColmanLuke J TallonJonathan H BadgerRamana MadupuNaomi L WardJonathan A EisenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 1, p e4207 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dongying Wu Jason Raymond Martin Wu Sourav Chatterji Qinghu Ren Joel E Graham Donald A Bryant Frank Robb Albert Colman Luke J Tallon Jonathan H Badger Ramana Madupu Naomi L Ward Jonathan A Eisen Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.
description	In order to enrich the phylogenetic diversity represented in the available sequenced bacterial genomes and as part of an "Assembling the Tree of Life" project, we determined the genome sequence of Thermomicrobium roseum DSM 5159. T. roseum DSM 5159 is a red-pigmented, rod-shaped, Gram-negative extreme thermophile isolated from a hot spring that possesses both an atypical cell wall composition and an unusual cell membrane that is composed entirely of long-chain 1,2-diols. Its genome is composed of two circular DNA elements, one of 2,006,217 bp (referred to as the chromosome) and one of 919,596 bp (referred to as the megaplasmid). Strikingly, though few standard housekeeping genes are found on the megaplasmid, it does encode a complete system for chemotaxis including both chemosensory components and an entire flagellar apparatus. This is the first known example of a complete flagellar system being encoded on a plasmid and suggests a straightforward means for lateral transfer of flagellum-based motility. Phylogenomic analyses support the recent rRNA-based analyses that led to T. roseum being removed from the phylum Thermomicrobia and assigned to the phylum Chloroflexi. Because T. roseum is a deep-branching member of this phylum, analysis of its genome provides insights into the evolution of the Chloroflexi. In addition, even though this species is not photosynthetic, analysis of the genome provides some insight into the origins of photosynthesis in the Chloroflexi. Metabolic pathway reconstructions and experimental studies revealed new aspects of the biology of this species. For example, we present evidence that T. roseum oxidizes CO aerobically, making it the first thermophile known to do so. In addition, we propose that glycosylation of its carotenoids plays a crucial role in the adaptation of the cell membrane to this bacterium's thermophilic lifestyle. Analyses of published metagenomic sequences from two hot springs similar to the one from which this strain was isolated, show that close relatives of T. roseum DSM 5159 are present but have some key differences from the strain sequenced.
format	article
author	Dongying Wu Jason Raymond Martin Wu Sourav Chatterji Qinghu Ren Joel E Graham Donald A Bryant Frank Robb Albert Colman Luke J Tallon Jonathan H Badger Ramana Madupu Naomi L Ward Jonathan A Eisen
author_facet	Dongying Wu Jason Raymond Martin Wu Sourav Chatterji Qinghu Ren Joel E Graham Donald A Bryant Frank Robb Albert Colman Luke J Tallon Jonathan H Badger Ramana Madupu Naomi L Ward Jonathan A Eisen
author_sort	Dongying Wu
title	Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.
title_short	Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.
title_full	Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.
title_fullStr	Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.
title_full_unstemmed	Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.
title_sort	complete genome sequence of the aerobic co-oxidizing thermophile thermomicrobium roseum.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/f00f2032cda24874b02937341995f6b2
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Complete genome sequence of the aerobic CO-oxidizing thermophile Thermomicrobium roseum.

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