Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds

Bioactive dressings are usually produced using natural or synthetic polymers. Recently, special attention has been paid to β-glucans that act as immunomodulators and have pro-healing properties. The aim of this research was to use β-1,3-glucan (curdlan) as a base for the production of bioactive dres...

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Autores principales:	Michal Wojcik, Paulina Kazimierczak, Vladyslav Vivcharenko, Malgorzata Koziol, Agata Przekora
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	β-1,3-glucan cell migration biomaterials skin regeneration matrix metalloproteinases Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f0106157f5894a2faef73a9ba6c43196
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spelling	oai:doaj.org-article:f0106157f5894a2faef73a9ba6c431962021-11-11T16:56:25ZEffect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds10.3390/ijms2221114741422-00671661-6596https://doaj.org/article/f0106157f5894a2faef73a9ba6c431962021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11474https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Bioactive dressings are usually produced using natural or synthetic polymers. Recently, special attention has been paid to β-glucans that act as immunomodulators and have pro-healing properties. The aim of this research was to use β-1,3-glucan (curdlan) as a base for the production of bioactive dressing materials (curdlan/agarose and curdlan/chitosan) that were additionally enriched with vitamin C and/or hydrocortisone to improve healing of chronic and burn wounds. The secondary goal of the study was to compressively evaluate biological properties of the biomaterials. In this work, it was shown that vitamin C/hydrocortisone-enriched biomaterials exhibited faster vitamin C release profile than hydrocortisone. Consecutive release of the drugs is a desired phenomenon since it protects wounds against accumulation of high and toxic concentrations of the bioactive molecules. Moreover, biomaterials showed gradual release of low doses of the hydrocortisone, which is beneficial during management of burn wounds with hypergranulation tissue. Among all tested variants of biomaterials, dressing materials enriched with hydrocortisone and a mixture of vitamin C/hydrocortisone showed the best therapeutic potential since they had the ability to significantly reduce MMP-2 synthesis by macrophages and increase TGF-β1 release by skin cells. Moreover, materials containing hydrocortisone and its blend with vitamin C stimulated type I collagen deposition by fibroblasts and positively affected their migration and proliferation. Results of the experiments clearly showed that the developed biomaterials enriched with bioactive agents may be promising dressings for the management of non-healing chronic and burn wounds.Michal WojcikPaulina KazimierczakVladyslav VivcharenkoMalgorzata KoziolAgata PrzekoraMDPI AGarticleβ-1,3-glucancell migrationbiomaterialsskin regenerationmatrix metalloproteinasesBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11474, p 11474 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	β-1,3-glucan cell migration biomaterials skin regeneration matrix metalloproteinases Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	β-1,3-glucan cell migration biomaterials skin regeneration matrix metalloproteinases Biology (General) QH301-705.5 Chemistry QD1-999 Michal Wojcik Paulina Kazimierczak Vladyslav Vivcharenko Malgorzata Koziol Agata Przekora Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds
description	Bioactive dressings are usually produced using natural or synthetic polymers. Recently, special attention has been paid to β-glucans that act as immunomodulators and have pro-healing properties. The aim of this research was to use β-1,3-glucan (curdlan) as a base for the production of bioactive dressing materials (curdlan/agarose and curdlan/chitosan) that were additionally enriched with vitamin C and/or hydrocortisone to improve healing of chronic and burn wounds. The secondary goal of the study was to compressively evaluate biological properties of the biomaterials. In this work, it was shown that vitamin C/hydrocortisone-enriched biomaterials exhibited faster vitamin C release profile than hydrocortisone. Consecutive release of the drugs is a desired phenomenon since it protects wounds against accumulation of high and toxic concentrations of the bioactive molecules. Moreover, biomaterials showed gradual release of low doses of the hydrocortisone, which is beneficial during management of burn wounds with hypergranulation tissue. Among all tested variants of biomaterials, dressing materials enriched with hydrocortisone and a mixture of vitamin C/hydrocortisone showed the best therapeutic potential since they had the ability to significantly reduce MMP-2 synthesis by macrophages and increase TGF-β1 release by skin cells. Moreover, materials containing hydrocortisone and its blend with vitamin C stimulated type I collagen deposition by fibroblasts and positively affected their migration and proliferation. Results of the experiments clearly showed that the developed biomaterials enriched with bioactive agents may be promising dressings for the management of non-healing chronic and burn wounds.
format	article
author	Michal Wojcik Paulina Kazimierczak Vladyslav Vivcharenko Malgorzata Koziol Agata Przekora
author_facet	Michal Wojcik Paulina Kazimierczak Vladyslav Vivcharenko Malgorzata Koziol Agata Przekora
author_sort	Michal Wojcik
title	Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds
title_short	Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds
title_full	Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds
title_fullStr	Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds
title_full_unstemmed	Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds
title_sort	effect of vitamin c/hydrocortisone immobilization within curdlan-based wound dressings on in vitro cellular response in context of the management of chronic and burn wounds
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f0106157f5894a2faef73a9ba6c43196
work_keys_str_mv	AT michalwojcik effectofvitaminchydrocortisoneimmobilizationwithincurdlanbasedwounddressingsoninvitrocellularresponseincontextofthemanagementofchronicandburnwounds AT paulinakazimierczak effectofvitaminchydrocortisoneimmobilizationwithincurdlanbasedwounddressingsoninvitrocellularresponseincontextofthemanagementofchronicandburnwounds AT vladyslavvivcharenko effectofvitaminchydrocortisoneimmobilizationwithincurdlanbasedwounddressingsoninvitrocellularresponseincontextofthemanagementofchronicandburnwounds AT malgorzatakoziol effectofvitaminchydrocortisoneimmobilizationwithincurdlanbasedwounddressingsoninvitrocellularresponseincontextofthemanagementofchronicandburnwounds AT agataprzekora effectofvitaminchydrocortisoneimmobilizationwithincurdlanbasedwounddressingsoninvitrocellularresponseincontextofthemanagementofchronicandburnwounds
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Effect of Vitamin C/Hydrocortisone Immobilization within Curdlan-Based Wound Dressings on In Vitro Cellular Response in Context of the Management of Chronic and Burn Wounds

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